Method for extracting and separating tryptophan from fermentation liquor

A technology of tryptophan and fermentation broth, applied in the field of separation and purification of amino acids, which can solve the problems of high production cost, complicated operation, and low purity

Pending Publication Date: 2021-10-22
国药集团健康产业研究院有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the technical problems of complex operation, high production cost, low product yield and low purity in the prior art, the present invention provides a method for extracting and separating L-tryptophan from fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 cation exchange resin crude extraction

[0031] 3.2L of tryptophan fermented liquid obtained by prior art fermentation, the fermented liquid was adjusted to pH=10.1 with 5M NaOH solution, centrifuged after fully stirring for 50min to obtain 2.3L of centrifuged supernatant, with a titer of 1450mg / L (3.34g ). Adjust the pH of the centrifuge to 3.5, and then adsorb it on 600ml of sodium D004 cation exchange resin, wash away some impurities and pigments with 5BV of pure water at a flow rate of 2BV / h, and elute with 5BV of 0.5M ammonia water at a flow rate of 1BV / h to obtain The crude extract of tryptophan with a purity of 72% was 3 L, the titer was 915 mg / L (2.745 g), and the yield was 84.19%.

Embodiment 2

[0032] Embodiment 2 cation exchange resin crude extraction

[0033] 3L of tryptophan fermentation liquid obtained by fermentation in the prior art, adjust the pH of the fermentation liquid to 10.3 with 5M NaOH solution, and centrifuge after fully stirring for 30 minutes to obtain 2.2L of centrifuged supernatant with a titer of 1250mg / L (2.75g) . Adjust the pH of the centrifuge to 3.3, and then adsorb it on 500ml of hydrogen-form D004 cation exchange resin, wash away some impurities and pigments with 4BV of pure water at a flow rate of 2BV / h, and elute with 5BV of 0.5M ammonia water at a flow rate of 1BV / h to obtain The crude extract of tryptophan with a purity of 55% was 2.5 L, the titer was 730 mg / L (1.825 g), and the yield was 66.36%.

Embodiment 3

[0034] Embodiment 3 cationic exchange resin crude extraction

[0035] 3.3L of tryptophan fermented liquid obtained by prior art fermentation, the fermented liquid was adjusted to pH=10.4 with 5M NaOH solution, centrifuged after fully stirring for 60min to obtain 2.5L of centrifuged supernatant, with a titer of 1400mg / L (3.5g ). Adjust the pH of the centrifuge to 3.5 and adsorb it on 600ml of ammonia-type D004 cation exchange resin, wash away some impurities and pigments with 4BV of pure water at a flow rate of 2BV / h, and elute with 5BV of 0.5M ammonia water at a flow rate of 1BV / h to obtain The crude extract of tryptophan with a purity of 68% was 3L, the titer was 820 mg / L (2.46 g), and the yield was 70.29%.

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PUM

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Abstract

The invention provides a method for extracting and separating tryptophan from fermentation liquor. The method comprises the following steps: pretreatment of fermentation liquor, crude extraction of cation exchange resin, purification of macroporous adsorption resin, decoloration and desalination, and freeze drying. The method is simple and convenient to operate, and the purity of the obtained sample is relatively high on the basis of ensuring high yield.

Description

technical field [0001] The invention relates to a method for separating and purifying amino acids, in particular to a method for extracting and separating and purifying L-tryptophan from fermentation broth. Background technique [0002] L-Tryptophan is one of the 20 common amino acids that make up proteins, and it is also one of the 8 essential amino acids necessary for humans and animals, also known as the second essential amino acid. Due to its special structure, biological function and the effect of its metabolites on the body, L-tryptophan has physiological functions such as regulating protein synthesis, improving immunity, enhancing 5-hydroxytryptophan metabolism, relieving tension, and improving sleep quality. Function plays a very important role in the growth and metabolism of humans and animals, so it is widely used in food, feed additives, medicine, agriculture and environmental monitoring. [0003] The earliest production of L-tryptophan mainly relied on chemical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D209/20
CPCC07D209/20
Inventor 张培燕胡海峰田勋王志娟
Owner 国药集团健康产业研究院有限公司
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