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Risk assessment device for prostate cancer

A technology for prostate cancer and risk assessment, applied in the field of biomedicine, can solve problems such as increased psychological burden on patients, achieve high compliance and avoid pollution

Pending Publication Date: 2021-10-22
SHENZHEN HUIXIN LIFE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Overtreatment often brings complications such as infection, sepsis and even death, and unnecessary invasive treatment will also have side effects, such as impotence and urinary incontinence, and the psychological burden on patients will also greatly increase

Method used

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  • Risk assessment device for prostate cancer
  • Risk assessment device for prostate cancer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] S1. Collect the test subject's urine, and purify the exosome solution from the urine.

[0073] Collect 5mL-50mL of urine from the test subjects, extract the exosome solution in it by ultrafiltration, the filter membrane has a pore size of 10-50nm, and finally obtain 100-500μl of purified exosome solution.

[0074] S2. Extracting RNA from the exosome solution obtained in S1 to obtain a sample RNA solution.

[0075] Cleavage the exosome solution by adding 200-1000 μl Trizol to the sample exosome solution, incubate the homogenate at room temperature (18-25°C) for 5-15 minutes, shake up and down for 15-30 seconds, and incubate at 2-8°C , Centrifuge at 8000-14000rpm for 2-15min, take the upper clear solution, put the upper clear solution on the RNeasy micro-column for RNA purification, and obtain the sample RNA solution.

[0076] S3. Take the sample RNA solution obtained in S2, add it to the qRT-PCR amplification system, first amplify at 30-50°C for 5-50 minutes, and then a...

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Abstract

The invention provides a risk assessment device for prostate cancer. The device comprises a collection device; a nucleic acid extraction device; an amplification device and a data acquisition and analysis device, wherein the data acquisition and analysis device is used for quantitatively detecting expression quantities of a PCA3 gene, an ERG gene and an SPDEF gene in a sample RNA solution and calculating expression quantities of the PCA3 gene and the ERG gene relative to the SPDEF gene, wherein delta CT of the PCA3 gene and the ERG gene is calculated by recording Ct values of the PCA3 gene, the ERG gene and the SPDEF gene, the sum of the relative expression quantities of the PCA3 gene and the ERG gene is calculated by using a calculation formula of the relative expression quantities, namely delta delta CT = 2^delta Ct (PCA3) + 2^delta Ct (ERG), and then a score is calculated by using a formula (16.92-delta delta Ct) * 1.83 and can reflect the risk level of the prostatic cancer.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a risk assessment device for prostate cancer. Background technique [0002] Globally, prostate cancer is the second most common malignancy in men after lung cancer. In recent years, with the aging of the population, changes in diet and lifestyle, improvement in diagnosis and treatment technology, and enhancement of health concepts, the incidence and diagnosis rate of prostate cancer have increased significantly. Early diagnosis is extremely important for prostate cancer, but there has been a lack of relevant diagnostic tools to distinguish high-grade and low-grade prostate cancer. Prostate-specific antigen (PSA) screening is currently the most commonly used test to determine whether a man should undergo a prostate biopsy, but it has a high rate of both false positives and false negatives and does not accurately predict the presence of invasive disease. However, most men with elevated...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6851
CPCC12Q1/6886C12Q1/6851C12Q2600/158C12Q2600/118C12Q2600/16C12Q2531/113C12Q2537/143C12Q2537/165
Inventor 陈欲超
Owner SHENZHEN HUIXIN LIFE TECH CO LTD
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