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Method for diagnosing alzheimer's disease using silver nanogap shell

A silver nanotechnology for Alzheimer's disease, applied in disease diagnosis, material inspection products, instruments, etc., can solve problems such as unreachable sensitivity, patient pain, and high cost

Pending Publication Date: 2021-10-22
IUCF HYU (IND UNIV COOP FOUNDATION HANYANG UNIV)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Previous methods required expensive equipment and were accompanied by patient suffering due to invasive CSF sampling
In addition, due to the inability to track the degree of disease progression, there is a fatal problem that it can only be diagnosed after the symptoms have progressed to a considerable extent
In addition, in order to track the onset and progression of AD with complex mechanisms, it is necessary to track multiple related biomarkers at the same time to improve the accuracy of disease diagnosis and the possibility of early diagnosis.
Furthermore, for early diagnosis, techniques for detecting various biomarkers from patient samples such as blood by non-invasive or minimally invasive methods are required, but there are extremely small amounts of AD target biomarkers at the pg / mL level in blood samples substances, requiring very sensitive diagnostic techniques to detect them, but previous techniques such as ELISA have had problems with the sensitivity

Method used

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  • Method for diagnosing alzheimer's disease using silver nanogap shell
  • Method for diagnosing alzheimer's disease using silver nanogap shell
  • Method for diagnosing alzheimer's disease using silver nanogap shell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1. Preparation of AgNGS-Aβ40 and AgNGS-Aβ42

[0047] Silver nanoslit shells into which antibodies specific for Aβ40 or Aβ42 were independently introduced were produced according to the method described below (see KR 10-1944346).

[0048] 1-1. Synthesis of silica particles and formation of thiol interaction groups

[0049] (1) Dissolve tetraethyl orthosilicate in 40ml of pure ethanol, add ammonium hydroxide, and react at room temperature for 20 hours.

[0050] (2) After removing unreacted substances with a centrifuge, the final dilution was to a concentration of 1 mg / ml.

[0051] (3) Add mercaptopropyltripolyethoxysilane and ammonium hydroxide to 1 ml of synthetic silica particles (1 mg / ml), and react at room temperature for 12 hours.

[0052] (4) Use a centrifuge to remove unreacted substances. (MPTS-silica)

[0053] 1-2. Formation of silver nano-cracked shells on the surface of silica particles using thiol-silver binding force

[0054] (1) MPTS-silica,...

Embodiment 2

[0064] Example 2. Detection of two kinds of Alzheimer's disease (AD) biomarkers (Aβ40, Aβ42)

[0065] figure 2 It is a figure showing the detection results of two kinds of Alzheimer's disease (AD) biomarkers (Aβ, Aβ) ((a) is a microarray mapping image for each concentration detected for Aβ, (b) is a microarray mapping image showing (a) is a graph of the AgNGS Raman signal for the region indicated, (c) is a graph of the signal for each concentration of (a), (d) is a microarray mapping image for each concentration of Aβ detection, (e) is For the Raman signal of AgNGS in the region shown in (d), (f) is a graph of the signal for each concentration of (d)).

[0066] if refer to figure 2 It was confirmed that Aβ40 and Aβ42 can be detected in a concentration-dependent manner by the AgNGS-based SERS immunoassay.

Embodiment 3

[0067] Example 3. Specificity test of AgNGS-based SERS immune response

[0068] image 3 is a graph showing the specificity test of the AgNGS-based SERS immune response.

[0069] image 3 (a) AgNGS modified with an antibody specific for each target reacts only with target biomarkers specifically for each target, and exhibits a Raman signal by immunoreaction. image 3 (b) When the concentration of Aβ42 is fixed at 10 ng / mL and the concentration of Aβ40 is changed to 0-10 ng / mL, for the mixed solution of the two biomarkers, the AgNGS-based SERS immunoassay shows the expression of the two biomarkers. Raman signal slope with concentration change. Additionally, if referring to image 3 (c), When Aβ40 and Aβ42 are present in the ratio on the x-axis, the AgNGS-based SERS immunoassay shows a propensity for Raman signals reflecting the concentration changes of the two biomarkers. From the corresponding results, it can be demonstrated that the AgNGS-based SERS immunoassay can spe...

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Abstract

The present invention relates to a method for diagnosing Alzheimer's disease by using a silver nanogap shell, the method enabling non-invasive early diagnosis of AD via multiplexed detection of a plurality of AD target biomarkers with high sensitivity.

Description

technical field [0001] The present invention relates to a method for diagnosing Alzheimer's disease using silver nanogapshell (AgNGS). More specifically, the present invention relates to a diagnostic method for Alzheimer's disease using silver nanoslit shell-based SERS immunoassay, and relates to a method that can multiplex multiplex detection of multiple Alzheimer's disease target biomarkers with high sensitivity and with A diagnostic method for the early diagnosis of Alzheimer's in a non-invasive manner. Background technique [0002] The clinical diagnosis of Alzheimer's disease (Alzheimer's Disease: AD) relies on brain imaging techniques such as PET, interrogation, and enzyme-linked immunosorbent assay (ELISA) such as Aβ40 and Aβ42 present in cerebrospinal fluid. Recently, for the accuracy and early diagnosis of AD, diagnostic methods based on various nanotechnology are being developed. In particular, SERS-based diagnostic methods have attracted considerable attention i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/553G01N33/68G01N21/65
CPCG01N33/54346G01N33/54326G01N33/553G01N33/54366G01N33/6896G01N21/658G01N2333/4709G01N2800/2821
Inventor 金悰浩梁眞京黄仁俊金慧仁
Owner IUCF HYU (IND UNIV COOP FOUNDATION HANYANG UNIV)
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