Application of Verticillium dahliae VdPRMT1 gene in improvement of disease resistance of crops or vegetables

A technology of Verticillium dahliae and genes, applied in the direction of genetic engineering, plant genetic improvement, application, etc., can solve problems such as difficult prevention and control

Active Publication Date: 2021-10-26
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since Verticillium dahliae is a soil-borne phytopathogenic fungus, it is very difficult to control it, and there is no effective medicament in production at present

Method used

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  • Application of Verticillium dahliae VdPRMT1 gene in improvement of disease resistance of crops or vegetables
  • Application of Verticillium dahliae VdPRMT1 gene in improvement of disease resistance of crops or vegetables
  • Application of Verticillium dahliae VdPRMT1 gene in improvement of disease resistance of crops or vegetables

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Embodiment 1

[0046] Embodiment 1 Verticillium dahliae VdPRMT1 Screening of target gene fragments for gene resistance to pathogenic bacteria, construction of interference vectors and application of transgenic tobacco for disease resistance

[0047] ⒈ Test material

[0048] ⑴ Materials

[0049] Tobacco: benthamiana ( Nicotiana benthamiana )strain.

[0050] Culture conditions: planted in mixed nutrient soil sterilized by high temperature and high pressure (Fangjie nutrient soil: vermiculite = 1:1), temperature 23 ± 2°C, relative humidity 75 ± 5%, photoperiod L:D is 16 h: 8 h.

[0051] ⑵ strains and plasmids

[0052] Cotton Verticillium dahliae pathogen: Verticillium dahliae ( Verticillium dahliae ) V991, a highly pathogenic deciduous strain, was donated by Jian Guiliang, a researcher at the Institute of Plant Protection, Chinese Academy of Agricultural Sciences.

[0053] Viral vector: tobacco rattle virus ( Tobacco rattle virus , TRV) binary vector (TRV1 and TRV2) was kindly donated ...

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Abstract

The invention discloses an application of a Verticillium dahliae VdPRMT1 gene in improvement of the disease resistance of crops. The method comprises the following steps of: constructing a plurality of tobacco rattle virus interference plasmids aiming at the Verticillium dahliae VdPRMT1 gene by taking a Verticillium dahliae strain as a material through host-induced gene silencing, converting Nicotina benthamiana, inoculating the Verticillium dahliae, and preliminarily determining that the VdPRMT1 gene in the Verticillium dahliae is associated with pathogenicity. The Verticillium dahliae VdPRMT1 is further taken as a target gene, and a target gene segment capable of remarkably improving the plant disease resistance is obtained through screening by combining transgenosis and an RNAi (Ribonucleic Acid Interference) technology. The screened target gene segment and a Gateway interference vector constructed by applying the target gene segment can be applied to the aspects of improving the resistance of plants to diseases caused by the Verticillium dahliae, cultivating new varieties of transgenic plants capable of resisting the Verticillium dahliae and the like.

Description

technical field [0001] The present invention relates to Verticillium dahlia protein arginine methyltransferase 1 gene ( VdPRMT1 ), especially involving Verticillium dahliae VdPRMT1 Use of genes for improving resistance of crops or vegetables to pathogenic hazards belonging to Verticillium dahliae VdPRMT1 New fields of use for genes. Background technique [0002] Verticillium Wilt of cotton is called "cotton cancer". In many countries, cotton production has been reduced by an average of 10-35%, which has seriously endangered cotton production and caused huge economic losses (Wang Xiaokun; Wang Chunyan; Xie Chengjian; Yang Xingyong, Advances in molecular mechanisms of Verticillium dahliae pathogenicity and plant resistance to Verticillium dahliae. Henan Agricultural Science . 2014, 43 (01), 1-6.). The pathogen is Verticillium dahliae ( Verticillium dahliae ), it has a strong pathogenicity, and it can infect cotton throughout the growth stage, causing wilting and yellow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N15/113C12N15/82A01H5/00A01H6/82A01H6/60A01H6/34A01H6/54
CPCC12N9/1007C12N15/1137C12N15/8218C12N15/8282C12Y201/01023C12N2310/14
Inventor 苏晓峰吴思源刘璐王琦程红梅
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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