Check patentability & draft patents in minutes with Patsnap Eureka AI!

Acoustic extracellular matrix hydrogels and their use

A hydrogel and exogenous matrix technology, applied in medical science, pharmaceutical formulations, prostheses, etc., can solve the problem of weakening the biological activity of ECM components

Active Publication Date: 2021-10-26
联邦高等教育系统匹兹堡大学
View PDF37 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ECM hydrogels prepared according to this technique inevitably undergo protein degradation and denaturation, thereby weakening the full complement of ECM molecules and the bioactivity of tissue-specific ECM components.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Acoustic extracellular matrix hydrogels and their use
  • Acoustic extracellular matrix hydrogels and their use
  • Acoustic extracellular matrix hydrogels and their use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0150] Materials and methods

[0151]Preparation of skin ECM: Skin ECM was prepared as previously described (Reing JE, et al. Biomaterials. 2010;31(33):8626-33). Briefly, full-thickness skin was harvested from market weight (approximately 110 kg) pigs (Tissue Source Inc.) and subcutaneous fat and epidermis were removed by mechanical stratification. The tissue was then treated with 0.25% trypsin (Thermo Fisher Scientific) for 6 hours, 70% ethanol for 10 hours, and 3% H 2 o 2 Treatment for 15 minutes, treatment with 1% TritonX-100 (Sigma-Aldrich) / 0.26% EDTA / 0.69% tris for 6 hours, solution exchange for another 16 hours, and treatment with 0.1% peracetic acid / 4% ethanol (Rochester Midland) for 2 hours . Water washes were performed between each chemical change, with alternating water and phosphate-buffered saline (PBS) washes following the final step. All chemical exposures were performed with stirring at 300 rpm on an orbital shaker. The skin ECM was then lyophilized and gro...

Embodiment 2

[0165] result

[0166] developed a method to prepare hydrogels from extracellular matrix (ECM). Using decellularized tissue as a starting material, sonication can be applied to a wide range of tissue-specific ECMs, including the dermis, bladder stroma (UBM), and small intestinal submucosa (SIS). The method involves resuspension of comminuted ECM in neutral buffered saline solution followed by ECM solubilization with, for example, 20 kHz ultrasound frequency, using an amplitude of 20-100% (Figure 1). After 60 seconds of sonication, rapid gelation of the ECM solution was induced by lowering the temperature of the ECM solution to below 37° C. ( FIG. 4 , FIG. 5 , FIG. 6 ). The results of the rheological evaluation showed that using this method, ECM hydrogels could be prepared using ECM concentrations ranging from 25 mg / ml to 150 mg / ml ( Figure 7 , Figure 8 ). Once polymerized, ECM gels are stable at room temperature and can conform to customizable 3D geometries (Figure 2). ...

Embodiment 3

[0168] Materials and methods for Examples 4-7

[0169] Preparation of ECM bioscaffolds: Porcine skin ECM (dECM) was prepared as previously described (Reing et al., Biomaterials 31(33) (2010) 8626-33). Briefly, full-thickness skin was harvested from pigs of market weight (approximately 110 kg), and subcutaneous fat and epidermis were removed by mechanical stratification. The tissue was then treated with 0.25% trypsin (Thermo Fisher Scientific) for 6 hours, 70% ethanol for 10 hours, and 3% H 2 o 2 Treatment for 15 minutes, treatment with 1% Triton X-100 (Sigma-Aldrich) / 0.26% EDTA / 0.69% tris for 6 hours, solution exchange for another 16 hours, and treatment with 0.1% peracetic acid / 4% ethanol (Rochester Midland) for 2 Hour. Water washes were performed between each chemical change, with alternating water and phosphate-buffered saline (PBS) washes following the final step. All chemical exposures were performed with stirring at 300 rpm on an orbital shaker. The skin ECM was the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
viscosityaaaaaaaaaa
Login to View More

Abstract

Methods are disclosed herein for producing a mammalian acoustic extracellular matrix (ECM) hydrogel. In further embodiments, mammalian acoustic ECM hydrogels are disclosed that are produced using the disclosed methods. Also disclosed is a mammalian acoustic ECM hydrogel, wherein the hydrogel is thermoreversible. Methods of using these acoustic ECM hydrogels are also disclosed.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Provisional Application 62 / 817,787, filed March 13, 2019, and U.S. Provisional Application 62 / 950,565, filed December 19, 2019, the entire contents of which are incorporated herein by reference. technical field [0003] The present invention relates to the field of hydrogels, in particular to acoustic ECM hydrogels fabricated from mammalian extracellular matrix (ECM) using ultrasound and uses thereof. Background technique [0004] Hydrogels composed of purified ECM components such as collagen, hyaluronic acid, silk fibroin, laminin, and fibronectin have been widely used in tissue engineering applications. However, these purified single-component ECM biomaterials lack the complex biochemistry of native tissue ECM. Decellularization of whole tissues or organs provides an alternative method of harvesting ECM that preserves the biochemistry of native tissue ECM. A major advance...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00
CPCA61L27/3633A61L27/3691A61L27/52A61L26/0057A61L26/008A61L2400/04A61L27/3683
Inventor S·F·巴迪拉克G·S·赫西
Owner 联邦高等教育系统匹兹堡大学
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com