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Application of SEA in preparation of drugs for treating inflammatory bowel diseases and biomarker for detecting curative effect of inflammatory bowel diseases

A technology of inflammatory bowel disease and biomarkers, which is applied in the field of biomarkers to detect the efficacy of inflammatory bowel disease, and can solve the problems of slow curative effect, easy drug resistance, and increased treatment of inflammatory bowel disease

Pending Publication Date: 2021-10-29
JIANGSU INST OF PARASITIC DISEASES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the treatment of IBD disease is still the treatment of antibacterial drugs combined with light diet, but the curative effect is slow, and long-term use is prone to drug resistance, which adds new difficulties to the treatment of inflammatory bowel disease

Method used

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  • Application of SEA in preparation of drugs for treating inflammatory bowel diseases and biomarker for detecting curative effect of inflammatory bowel diseases
  • Application of SEA in preparation of drugs for treating inflammatory bowel diseases and biomarker for detecting curative effect of inflammatory bowel diseases
  • Application of SEA in preparation of drugs for treating inflammatory bowel diseases and biomarker for detecting curative effect of inflammatory bowel diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Construction of a mouse model of intestinal inflammation and administration methods (see figure 1 )

[0037] 1. Grouping and administration of mice:

[0038] Forty mice (body weight 26.22±1.12g) were randomly divided into 4 groups, namely control group (10 mice), SEA50μg group (10 mice), DSS group (10 mice), DSS+SEA50μg group (10 mice).

[0039]On the 0th day of the experiment, the DSS group and the SEA50μg+DSS group were given 3% DSS (3g DSS per 100ml of drinking water) instead of normal drinking water. And on the 0th day of DSS modeling, each mouse in the SEA50μg group and SEA50μg+DSS group was given intraperitoneal injection of SEA50μg, while the control group had a normal diet.

[0040] The modeling method and the criteria for verifying the successful modeling can be found in the prior art: [1] BAUER C, DUEWELL P, MAYERC, et al. Colitis induced in mice with dextran sulfate sodium(DSS) is mediated by the NLRP3 inflammasome[J]. Gut , 2010, 59(9):1192-1199.

[0041...

Embodiment 2

[0062] The feces of the mice on the 10th day of the experiment in Example 1 were used as materials, and the 16s rRNA universal primer was used to amplify and sequence the intestinal flora to explore the relationship between SEA, ulcerative colitis and intestinal flora. relation.

[0063] The bioinformatics analysis of the sequencing data is carried out as follows:

[0064] Raw data is in FASTQ format. Using Trimmomatic [1] The software de-scrambles the original paired-end sequence. The impurity removal parameters are: detect and truncate the ambiguous base N; and use the sliding window method to check the average base quality. When the quality is lower than 20, the previous high-quality sequence is intercepted. The double-ended sequence after decontamination utilizes FLASH [2] software. The splicing parameters are as follows: the smallest overlap is 10 bp, the largest overlap is 200 bp, and the largest mismatch rate is 20%.

[0065] To ensure the accuracy of the results,...

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Abstract

The invention provides application of SEA in preparation of drugs for treating inflammatory bowel diseases and a biomarker for detecting prognosis of the inflammatory bowel diseases, and belongs to the technical field of drugs for treating the inflammatory bowel diseases. Experiments prove that the schistosoma japonicum soluble egg antigen (SEA) achieves the purpose of treating the inflammatory bowel diseases by relieving colon shortening, reducing disease activity indexes, relieving inflammation symptoms and inhibiting inflammatory response, and therefore the application of the schistosoma japonicum soluble egg antigen in preparing the drugs for treating the inflammatory bowel diseases is provided. The difference intestinal flora and metabolite related to enteritis between a treatment group and a model group are further analyzed, and the screened Helicobacter sp., the screened Prevoteraceae sp. and the screened lacnostostridiumsp. can be used as biomarkers to be applied to the evaluation of the curative effect of the inflammatory bowel diseases.

Description

technical field [0001] The invention belongs to the technical field of inflammatory medicines, and particularly relates to the application of SEA in the preparation of medicines for treating inflammatory bowel disease and a biomarker for detecting the curative effect of inflammatory bowel disease. Background technique [0002] Inflammatory bowel disease (IBD) is a chronic relapsing inflammatory bowel disease with unclear etiology, including 3 kinds of ulcerative colitis (UC), Crohn's disease (CD), indeterminate colitis ( IC). At present, the incidence of IBD is increasing, and its pathogenesis may be related to genes, environment, immunity and intestinal flora. A large number of flora, fungi and viruses colonize the human gut, and their dysregulation may play a very important role in the pathogenesis of IBD. At the same time, dysbacteriosis causes intestinal metabolic dysfunction. [0003] At present, the treatment of IBD is still based on antibiotics combined with a ligh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/002A61P1/00A61P29/00A61P1/04C12Q1/6883C12Q1/6895
CPCA61K39/002A61P1/00A61P29/00A61P1/04C12Q1/6883C12Q1/6895C12Q2600/118C12Q2600/158Y02A50/30
Inventor 黄玉政朱恬宇
Owner JIANGSU INST OF PARASITIC DISEASES
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