CRE drug-resistant gene preliminary typing detection kit and use method thereof

Abstract

The invention discloses a CRE drug-resistant gene preliminary typing detection kit and a use method thereof. The CRE drug-resistant gene preliminary typing detection kit comprises a plate body, a bottle body is embedded in the top of the plate body, an upper-layer container and a lower-layer container are arranged in the bottle body, a first injection opening is formed in the top of the upper-layer container, a second injection opening is formed in the top of the lower-layer container, detection holes are formed in the top of the plate body and comprise a first drug sensitive hole and a second drug sensitive hole, and drug-resistant genotypes are identified and screened according to the principle that culture and biochemical reagent antibacterial drugs synergistically inhibit carbapenemase; nutrient substances required by growth and metabolism of the target microorganisms are added into the upper-layer container and the lower-layer container, and meanwhile, micromolecular products metabolized during growth of the target microorganisms are detected by using a culture drug sensitive agent consisting of a color indicator solution and an antibacterial drug; the metabolite can change the color of the upper container and the lower container so as to judge whether target microorganisms exist in the sample or not.

Description

technical field [0001] The invention relates to the technical field of microbial detection, in particular to a CRE drug resistance gene preliminary typing detection kit and a use method thereof. Background technique [0002] CRE is a carbapenem-resistant Enterobacteriaceae. Its main characteristics are strong drug resistance, strong virulence, rapid spread, and different treatment options for different drug-resistant genotypes. And the mortality rate caused by infection is high. Therefore, there is a need for a rapid method to determine whether there is such a bacterial infection, and to initially distinguish its drug-resistant genotypes. The most common resistance mechanism is the production of carbapenems, and the most common types of carbapenems are KPC enzymes and metalloenzymes. The most widely used microbial detection method for drug-resistant genes is mainly the PCR method, which is sensitive and accurate, and can simultaneously provide specific typing of this type...

AI Technical Summary

Problems solved by technology

[0003] 1. It takes a long time, from the formation of colonies to the extraction of bacterial DNA, PCR amplification takes 2 days;

[0004] 2. The operation is cumbersome. A lot of disinfection, sterilization and cleaning work are required in the preparation of the test sterile container, the preparation of the PCR reaction system, and the preparation of related consumables for gel electrophoresis, which will consume a lot of manpower and material resources; The technical level requirements are high, and human errors are prone to occur;

[0005] 3. The detection results are lagging behind, and detection information cannot be provided quickly and in real time, which is not conducive to providing rapid infection evidence to the clinic in a timely manner. Although the PCR method has improved in recent years, it is still impossible to obtain the detection results in real time and cannot meet the clinical needs of use.

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