Method for separating and extracting cytidine from fermentation liquor

A technology of cytidine and permeate is applied in the field of separation and extraction of cytidine, which can solve the problems of few reports on the large-scale separation and purification process of cytidine, and achieve the effects of saving operation time, low energy consumption and high yield.

Pending Publication Date: 2021-11-09
NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the microbial fermentation synthesis method is the main method. There are many reports on how to breed efficient and high-yield microbial strains, but there are few research reports on the large-scale separation and purification process of cytidine.

Method used

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  • Method for separating and extracting cytidine from fermentation liquor
  • Method for separating and extracting cytidine from fermentation liquor
  • Method for separating and extracting cytidine from fermentation liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] (1) Adjust the pH of the fermented liquid containing cytidine to be 2, filter through a 50nm ceramic membrane, collect the permeate, and wash the retentate with tap water until the retentate does not contain cytidine, merge to obtain the permeate through the ceramic membrane .

[0066] (2) Load the ceramic membrane permeate liquid obtained in step (1) to 215 g of modified cation exchange resin NH-1, carry out adsorption, wash impurities, elute, and collect the eluate; the sample loading rate is 1BV / h , the loading sample amount is the cytidine fermentation broth clear liquid containing the total mass of 64g; during the washing process, the concentration of ammonia water is 0.01mol / L, the flow rate is 1BV / h, and the volume of washing impurities is 8BV; during the washing process, ammonia water The concentration is 0.05mol / L, the flow rate is 1BV / h, and the eluted volume is 14BV.

[0067] (3) Filter the eluate obtained in step (2) through a 300 Da nanofiltration membrane...

Embodiment 2

[0091] (1) Adjust the pH of the fermented liquid containing cytidine to be 3.5, filter through a 200nm ceramic membrane, collect the permeate, and wash the retentate with tap water until the retentate does not contain cytidine, merge to obtain the permeate through the ceramic membrane .

[0092] (2) Load the ceramic membrane permeate solution obtained in step (1) to 215g of modified cation exchange resin NH-1, carry out adsorption, wash impurities, elute, and collect the eluate; the sample loading rate is 1.5BV / h, the amount of sample loaded is cytidine fermentation broth clear liquid containing a total mass of 64g; during the washing process, the concentration of ammonia water is 0.05mol / L, the flow rate is 1.5BV / h, and the volume of washing is 10BV; , the concentration of ammonia water is 0.1mol / L, the flow rate is 2BV / h, and the eluted volume is 12BV.

[0093] (3) Filter the eluate obtained in step (2) through an 800 Da nanofiltration membrane to remove macromolecular imp...

Embodiment 3

[0099] (1) Adjust the pH of the fermented liquid containing cytidine to be 2.5, filter through a 100nm ceramic membrane, collect the permeate, and wash the retentate with tap water until the retentate does not contain cytidine, merge to obtain the permeate through the ceramic membrane .

[0100] (2) Load the ceramic membrane permeate obtained in step (1) to 215 g of the modified cation exchange resin NH-1, perform adsorption, wash impurities, elute, and collect the eluate; the sample loading rate is 2BV / h , the loading sample amount is the fermented liquid that contains 64g cytidine; During the washing process, the concentration of ammonia water is 0.03mol / L, the flow rate is 2BV / h, and the volume of washing impurities is 14BV; during the washing process, the concentration of ammonia water is 0.06 mol / L, the flow rate is 1.5BV / h, and the eluted volume is 13BV.

[0101] (3) Filter the eluate obtained in step (2) through a 500 Da nanofiltration membrane to remove macromolecular...

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Abstract

The invention discloses a method for separating and extracting cytidine from fermentation liquor. The method comprises the following steps of adsorbing and eluting liquid containing cytidine by cation exchange resin, and concentrating and crystallizing the obtained eluent to obtain cytidine crystals, wherein the cation exchange resin is obtained by modifying NH-1 resin with silver sulfate and / or sulfuric acid. The cation exchange column used in the method is modified NH-1 resin, the cytidine adsorption capacity of the cation exchange column reaches 0.3 g / g, the resin dosage is reduced, and the acid and alkali dosage and the operation time are indirectly saved.

Description

technical field [0001] The invention belongs to the technical field of biochemical separation, and in particular relates to a method for separating and extracting cytidine from fermentation broth. Background technique [0002] Cytidine is a pyrimidine nucleoside, an intermediate of citicoline, and an intermediate of many antiviral, antitumor, and antiAIDS drugs. Cytidine is widely used in many industries such as food, health products, cosmetics and medicine. Cytidine is especially an important drug synthesis intermediate in contemporary medicine. With the continuous expansion of its application range, the demand for cytidine is also increasing. Naturally, the research on the preparation of cytidine is also getting more and more in-depth. There is an urgent need to develop a cheap and scalable separation and purification process for the production of cytidine nucleosides. [0003] At present, the production of cytidine at home and abroad mainly includes chemical synthesis, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/06C07H1/06
CPCC07H19/06C07H1/06
Inventor 应汉杰陈勇温庆仕魏荷芬周精卫杨玉晨
Owner NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD
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