Kit for detecting polymorphism of folic acid metabolism genes

A folic acid metabolism gene and polymorphism detection technology, applied in the field of biomedicine, can solve the problems of insufficient folic acid utilization ability of pregnant women, etc., and achieve the effect of low cost and simple operation

Pending Publication Date: 2021-11-12
广东元盛医学检验有限公司
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AI Technical Summary

Problems solved by technology

[0004] Pregnant women generally take one folic acid tablet (400ug) per day to supplement folic acid, which is sufficient for pregnant...

Method used

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  • Kit for detecting polymorphism of folic acid metabolism genes
  • Kit for detecting polymorphism of folic acid metabolism genes
  • Kit for detecting polymorphism of folic acid metabolism genes

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Embodiment Construction

[0018] In order to explain in detail the technical content, structural features, achieved goals and effects of the technical solution, the following will be described in detail in conjunction with specific embodiments and accompanying drawings.

[0019] 1. Use commercial kits to extract genomic DNA from human blood

[0020] Extract DNA from dried blood spots or whole blood using Magnetic Bead Method Dried Blood Spot Genomic DNA Extraction Kit (TIANGEN, DP344-T3), Whole Blood Genomic DNA Extraction Kit (GenMagBio, NA001-1) or similar products. Use an ultraviolet spectrophotometer to perform quality inspection and quantification, then dilute the DNA concentration that passes the quality inspection to 10 ng / L, and store it at 4°C for later use.

[0021] 2. ARMS-PCR primer design

[0022] The invention designs ARMS-PCR specific primers with double mismatched bases for the 3SNP site of the folic acid metabolism gene. The 3' terminal bases of the two specific forward primers at ea...

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Abstract

The invention discloses a kit for detecting polymorphism of folic acid metabolism genes. The kit comprises a PCR premix solution for detecting three SNP sites of folic acid metabolism genes MTHFR and MTRR, ARMS-PCR amplification primers and a quality control product. Whether the folic acid metabolism genes have mutation or not is judged by detecting the three SNP sites of the MTHFR and the MTRR, the kit is used for evaluating the folic acid metabolism capacity of individuals and specifically prompting the folic acid taking amount of a detected person, genotypes of the three mutation sites can be determined during use, and the kit has the technical advantages of rapidness, accuracy, low cost and simplicity in operation.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and is used for evaluating the strength of an individual's ability to metabolize folic acid, and specifically prompting the amount of folic acid taken by a subject, in particular to a kit for detecting polymorphisms of folic acid metabolizing genes. Background technique [0002] Folic acid is a B vitamin that is necessary for the growth and division of cells in the body. The human body cannot synthesize folic acid by itself, and the folic acid ingested from food is reduced to tetrahydrofolate by substances such as 5,10-methylenetetrahydrofolate reductase (MTHFR) and methionine synthase reductase (MTRR). Folic acid can participate in many important biochemical metabolic reactions in the human body, and is a key cofactor in the synthesis of bases in the human body. [0003] The first trimester is a critical period for the differentiation of fetal organ systems and the formation of the placenta....

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6858
CPCC12Q1/6883C12Q1/6858C12Q2600/156C12Q2600/106C12Q2531/113C12Q2535/137C12Q2545/113
Inventor 朱正凯戴亦亨罗栩强郑元盛
Owner 广东元盛医学检验有限公司
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