Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of product for detecting GALNT4 gene in preparation of tool for diagnosis and/or prognosis of coronary heart disease

A coronary heart disease and gene technology, applied in the field of genetic diagnosis and biopharmaceuticals, can solve the problems such as myocardial infarction that cannot be accurately predicted and prevented, and many causes of coronary heart disease.

Inactive Publication Date: 2021-11-16
BEIJING FRIENDSHIP HOSPITAL CAPITAL MEDICAL UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Coronary heart disease and other chronic diseases are also complex polygenic diseases with multiple genes acting together. Because of the many causes of coronary heart disease and the pathogenesis has not been fully elucidated, until now, it has not been possible to accurately predict and prevent the occurrence of myocardial infarction. There is a need for more effective detection methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of product for detecting GALNT4 gene in preparation of tool for diagnosis and/or prognosis of coronary heart disease
  • Application of product for detecting GALNT4 gene in preparation of tool for diagnosis and/or prognosis of coronary heart disease
  • Application of product for detecting GALNT4 gene in preparation of tool for diagnosis and/or prognosis of coronary heart disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1: Case Collection

[0065] 1. Selected crowd

[0066] From August 2019 to November 2017, patients who were hospitalized in Beijing Friendship Hospital affiliated to Capital Medical University and received coronary angiography were enrolled. A total of 58 patients with coronary heart disease and 55 patients in the control group who were admitted to the hospital during the same period were excluded from the diagnosis of coronary heart disease.

[0067] 2. Testing standards

[0068] The diagnostic criteria for coronary heart disease are left main and three subepicardial coronary arteries (anterior descending, left circumflex, and right coronary arteries) and any segment of their large branches with a diameter stenosis of ≥50%.

[0069] 3. Selection criteria

[0070] (1) Aged between 18-80 years old (including 18 and 80 years old);

[0071] (2) LVEF ≥ 45% confirmed by transthoracic echocardiography;

[0072] (3) Voluntarily accept all inspections required by th...

Embodiment 2

[0082] Example 2: Detection of GALNT4 gene expression in peripheral blood of coronary heart disease and control group

[0083] 1. Collect peripheral blood and extract mRNA

[0084] (1) Take 5ml of fasting peripheral venous blood from each research subject in the morning, and centrifuge to collect blood cells;

[0085] (2) Add Trizol reagent to fully grind cells or tissue until it becomes paste;

[0086] (3) Add 1 / 5 volume of chloroform, shake vigorously up and down and mix for 15s, stand at room temperature for 15min, centrifuge at 12000g for 15min at 4°C;

[0087] (4) Carefully take out the EP tube from the centrifuge, at this time the homogenate is divided into 3 layers, that is, the colorless supernatant is RNA, the white egg layer in the middle and the lower organic phase with color; carefully suck the upper aqueous phase Transfer to another new EP tube, do not aspirate the white intermediate layer;

[0088] (5) Add equal volume of isopropanol to the supernatant, invert u...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of a product for detecting a GALNT4 gene in preparation of a tool for diagnosis and / or prognosis of a coronary heart disease. According to the application, by detecting the expression of the GALNT4 in a subject, whether the subject suffers from the coronary heart disease or not can be judged, or whether the subject has the risk of suffering from the coronary heart disease can be judged, or whether the prognosis of the subject is good can be judged, so that a clinician is guided to provide an effective prevention means or treatment scheme for the subject; and the survival rate of the subjects is favorably improved.

Description

technical field [0001] The invention relates to the technical field of gene diagnosis and biopharmaceuticals, in particular to the application of a polypeptide acetylgalactose aminotransferase 4 (pp-GalNAc-T4, GALNT4) gene in the diagnosis and prognosis tool of coronary heart disease. Background technique [0002] Coronary atherosclerotic heart disease (CHD) is one of the main diseases that threaten human health, and has the characteristics of high morbidity and high mortality. According to statistics, the number of coronary heart disease interventional treatments in China in 2019 is expected to exceed 1.1 million. Despite the development and promotion of a series of new technologies represented by percutaneous coronary interventional treatment technology, the prevalence of coronary heart disease in my country in the past few years has Therefore, a deep understanding of the etiological mechanism of coronary heart disease is of great significance for the prevention and treatme...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68C12N15/11
CPCC12Q1/6883G01N33/6893C12Q2600/118G01N2800/324
Inventor 黄榕翀叶智帅徐鸣悦李泽亚
Owner BEIJING FRIENDSHIP HOSPITAL CAPITAL MEDICAL UNIV