Protection solution suitable for normal-temperature preservation of excrement sample

Abstract

The invention discloses a protection solution suitable for normal-temperature preservation of an excrement sample. The protection solution comprises 50-60% (v / v) of ethanol, 1-1.5% (w / v) of citrate, 10-20 mM Tris-HCl, 2-3% (w / v) of EDTA-Na2, 3% (w / v) of sodium chloride and 0.8-1.2% (w / v) of trichlorobutanol. Compared with an existing product, the protection solution of the invention has the following characteristics that sample microorganism composition can be stabilized for a long time under a room temperature condition, DNA in a sample can be stored, the current situation that an existing sampling method extremely depends on low-temperature storage and transportation is effectively improved, sampling at home by a sample supplier who is inconvenient to move is greatly facilitated, and the accuracy of an excrement sample detection result is ensured. Practical use verifies that after anexcrement sample is preserved for 15 days at normal temperature by the protection solution, the flora type and abundance in the sample are slightly different from those of a fresh sample, and when the protection solution is applied to detection of 16s rRNA, biochips and the like, the consistency of detection data of the sample preserved for 15 days and the fresh sample meets the result precision requirement.

Description

technical field [0001] The invention relates to the technical field of microbial molecular detection, in particular to a protective solution suitable for storing feces samples at room temperature. Background technique [0002] Microorganisms such as bacteria, protozoa, and viruses that are symbiotic or parasitic with the human body in the intestinal tract are called the "second genome" of human beings by the scientific community. The "second genome" has a large number of bacteria with a group basis, and the distribution balance between species is particularly important for human health. In recent years, a large number of research results on intestinal flora have emerged in various top journals. Some common diseases include diabetes, asthma, chronic diarrhea, irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), obesity , low immunity, etc. have been found to be closely related to the disorder of the balance of bacterial distribution. With the continuous deepeni...

AI Technical Summary

Problems solved by technology

[0005] At present, the most commonly used method in hospitals and testing institutions is the cold storage method, that is, the collected human stool samples are stored in containers at -20°C or lower. Although this storage method is effective, it has a greater impact on transportation conditions and whether it can be refrigerated in time. High requirements, and some scenarios such as out-of-home sampling and sample provider’s home sampling are difficult to achieve. It can only be applied to small-scale experimental sampling, and it is difficult to scale commercial applications

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