SSR molecular marker primers and method for identifying scutellaria baicalensis in northeast region

Abstract

The invention provides SSR (simple sequence repeat) molecular marker primers and method for identifying scutellaria baicalensis in the northeast region, and the method comprises the following steps: taking total DNA (deoxyribonucleic acid) of a scutellaria baicalensis sample to be identified as a template, and performing PCR (polymerase chain reaction) amplification by using the SSR molecular marker primers to obtain an amplification product; performing capillary electrophoresis sequencing typing on the amplification product to obtain a typing result of the amplification product; carrying out peak map interpretation on the typing result of the amplification product by using GeneMarker 4.0 to obtain an allele matrix; according to the allele matrix, carrying out GenALEx calculation to obtain genetic diversity data of the scutellaria baicalensis; and on the basis of genetic diversity data obtained by GenALEx calculation, carrying out UPGMA clustering analysis through MEGA to construct a clustering tree. A genome technology and an SSR molecular marker technology are applied, the SSR primers are designed by utilizing the scutellaria baicalensis genome, the SSR primers are screened through scutellaria baicalensis samples in Hebei, Shanxi, Shandong, Shaanxi, Inner Mongolia, Liaoning, Jilin and the like, and the primers are used for specifically identifying scutellaria baicalensis samples in the northeast region, are high in accuracy and stability and are easy to popularize and apply in a large scale.

Description

technical field [0001] The invention belongs to the field of molecular biology molecular markers, in particular to an SSR molecular marker primer and method for identifying Scutellaria baicalensis in northeast China. Background technique [0002] Scutellaria baicalensis is a widely used traditional Chinese medicinal material. Its root is used as medicine. It is bitter in taste and cold in nature. Indications for febrile disease, upper respiratory tract infection, lung-heat cough, damp-heat jaundice, pneumonia, dysentery, hemoptysis, red eyes, restless fetus, high blood pressure, carbuncle, swollen boils and other diseases. The clinical antibacterial activity of Scutellaria baicalensis is better than that of Coptis chinensis, and it does not produce drug resistance. According to relevant statistics, 70% of Chinese patent medicines currently on the market contain Scutellaria baicalensis, such as commonly used Chinese patent medicines Qingkailing oral liquid, Shuanghuanglian g...

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defects of the prior art, the present invention provides a SSR molecular marker primer and method for identifying Scutellaria baicalensis in Northeast China, which solves the problem of using methods such as character identification and microscopic identification in the identification of the existing scutellaria baicalensis origin, relying on personal experience and subjective judgment, accurate Technical problems with low reliability and poor technology scalability

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