Clove essential oil Pickering emulsion coating preservative and preparation method and use thereof
A technology of Pickering emulsion and coating preservative, which is applied in application, fruit and vegetable preservation, and food ingredients as coating agent, etc. It can solve the problems of low volatility, low bacteriostasis and antioxidant activity of clove essential oil, Insufficient bactericidal effect and other problems, to achieve the effect of reducing volatilization loss, strong bacteriostasis, and wide application range
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[0036] see figure 1 Shown, be clove essential oil Pickering emulsion coating preservative preparation technology roadmap, the present invention proposes a kind of preparation method of clove essential oil Pickering emulsion coating preservative, comprise the following steps,
[0037] Step S1, prepare 20 mg / ml zein solution with 70%-80% ethanol and zein powder, prepare 0.4-6 mg / ml sodium caseinate solution with ultrapure water and sodium caseinate, and magnetically stir 6-12h;
[0038] Step S2, drop 1ml of 20mg / ml zein solution into 10ml of 0.4-6mg / ml sodium caseinate solution dropwise, and stir magnetically at 500rpm for 30min;
[0039] Step S3, using a rotary evaporator to evaporate the ethanol in the solution at 40°C, and replace the evaporated ethanol with ultrapure water to keep the volume of the solution unchanged;
[0040] Step S4, then add 0.5%-2% v / v clove essential oil, 15000-20000rpm high-speed homogenization for 3min, to obtain clove essential oil Pickering emulsi...
Embodiment 1
[0044] Embodiment 1—control group:
[0045] The preparation method of clove essential oil Pickering emulsion coating preservative:
[0046] Step S1, 1% v / v acetic acid and chitosan prepare 1.5% w / v chitosan solution, add 20% w / w glycerin into the chitosan solution. Homogenize at 15,000 rpm for 3 minutes, and defoam with ultrasonic waves for 30 minutes to obtain a pure chitosan film (control group) preservative.
Embodiment 2
[0048] The preparation method of clove essential oil Pickering emulsion coating preservative:
[0049] Step S1, prepare 20 mg / ml zein solution with 75% ethanol and zein; prepare 4 mg / ml sodium caseinate solution with ultrapure water and sodium caseinate, and magnetically stir for 12 hours.
[0050] Step S2, 1 ml of 20 mg / ml zein solution was dropped dropwise into 10 ml of 4 mg / ml sodium caseinate solution, and magnetically stirred at 500 rpm for 30 min.
[0051] Step S3, using a rotary evaporator to evaporate ethanol in the solution at 40° C., and replace the evaporated ethanol with ultrapure water to keep the volume of the solution unchanged.
[0052] Step S4, then add 1% v / v clove essential oil, and homogenize at 15000 rpm for 3 minutes to obtain clove essential oil Pickering emulsion.
[0053] Step S5, 1% v / v acetic acid and chitosan prepare 1.5% w / v chitosan solution, add 20% w / w glycerin into the chitosan solution. Then add clove essential oil Pickering emulsion to the ...
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