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Application of tert-butylhydroquinone in preparation of drugs for preventing and/or treating mucositis

A kind of technology of tert-butyl hydroquinone and mucositis, which is applied in the field of pharmacy

Pending Publication Date: 2021-11-30
成都医学院第一附属医院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of tert-butylhydroquinone to treat mucositis caused by chemotherapy

Method used

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  • Application of tert-butylhydroquinone in preparation of drugs for preventing and/or treating mucositis
  • Application of tert-butylhydroquinone in preparation of drugs for preventing and/or treating mucositis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: TBHQ alleviates 5-FU-induced damage to human intestinal epithelial cells

[0027] 1. Experimental method

[0028] In order to analyze the chemotherapeutic protective effect of TBHQ, the present invention first adopts different concentrations of chemotherapeutic drug 5-fluorouracil (5-FU) to treat human intestinal epithelial cells (HIEC cells), and screen out the optimal modeling concentration of 5 μM 5-FU, The 50% lethal dose of HIEC cells occurs when 5μM 5-FU is treated for 48 hours ( figure 1 A). Then HIEC cells were pretreated with different concentrations of TBHQ, and 5 μM 5-FU was added at the same time. After 48 hours, the release of lactate dehydrogenase (LDH) was measured with CytoTox 96 kit, and the cell viability was measured with CCK-8 detection kit.

[0029] Apoptosis test: HIEC cells were collected, stained with 7-AAD (2 μg / mL, Jiangsu Kaiji, China) for 20 minutes, and washed three times. A portion of the cells was then analyzed using a flow ...

Embodiment 2

[0033] Example 2: TBHQ alleviates 5-FU-induced intestinal mucositis in mice

[0034] 1. Experimental method

[0035] The present invention has studied the influence of TBHQ on the intestinal mucositis induced by 5-FU in mice ( figure 2 ).

[0036] The experimental operation is as follows:

[0037] Male C57 / BL6 mice (8 weeks, 18-20 g) were purchased from Chengdu Dashuo Experimental Animal Co., Ltd. (Sichuan, China). All mice were housed at 25°C with a 12-h light / dark cycle and kept in plastic cages with free access to food and water. The mice were randomly divided into three groups (n=6 / group): control group, 5-FU group, 5-FU+TBHQ group. The 5-FU group and the 5-FU+TBHQ group were injected intraperitoneally with 50 mg / kg 5-FU for 5 days to induce intestinal mucositis. At the same time, the 5-FU group and the 5-FU+TBHQ group were intraperitoneally injected with the same amount of DMSO or TBHQ (TBHQ was dissolved in DMSO, 10 mg / kg), once a day for 8 days (Day 1-8). Body we...

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Abstract

The invention provides application of tert-butylhydroquinone in preparation of drugs for preventing and / or treating mucositis, and belongs to the field of pharmacy. That tert-butylhydroquinone can significantly improve 5-FU induced intestinal mucositis, can improve diarrhea, reduce intestinal length loss, reduce pathological changes (such as mucosal injury, necrosis, tissue structure loss, edema and inflammatory cell infiltration), and reduce the expression level of inflammatory factors is found for the first time. That tert-butylhydroquinone has a protective effect on 5-FU induced human intestinal epithelial cell injury is also found. Therefore, TBHQ has a good application prospect in preparation of drugs for preventing and / or treating mucositis caused by chemotherapy and preparation of drugs having a protective effect on human intestinal epithelial cell injury.

Description

technical field [0001] The invention belongs to the field of pharmacy, and in particular relates to the use of tert-butylhydroquinone in the preparation of medicines for preventing and / or treating mucositis. Background technique [0002] Chemotherapy is one of the main methods to treat malignant tumors, but studies have found that the application of methotrexate (methotrexate, MTX), 5-fluorouracil (5-nuofine urea pyrimidine, 5-FU), cytarabine, vincristine, When chemotherapy drugs such as poside are used, the incidence of mucositis is as high as 90%. The occurrence of chemotherapeutic mucositis not only leads to increased treatment costs and prolonged hospital stay, but also increases the physical and mental pain of patients and reduces the quality of life of patients. Therefore, it is of great significance to develop effective drugs for the treatment of chemotherapeutic mucositis. [0003] The incidence of gastrointestinal mucositis after chemotherapy is 40%. Chemotherapy-...

Claims

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Application Information

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IPC IPC(8): A61K31/05A61P1/00A61P29/00
CPCA61K31/05A61P1/00A61P29/00
Inventor 许颖常晓松吴东明邓仕华刘腾
Owner 成都医学院第一附属医院
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