Tumor cell rapid detection electrochemical biosensor and preparation method and application thereof

Abstract

The invention discloses a tumor cell rapid detection electrochemical biosensor which comprises a flexible screen-printed electrode and gold nanoparticles deposited on the surface of the flexible screen-printed electrode. The surfaces of the gold nanoparticles are modified with thiolated folic acid molecules, and the surface of the flexible screen-printed electrode is sealed by bovine serum albumin; or the biosensor comprises a flexible screen-printed electrode and a gold nanoparticle-sulfhydrylated folic acid molecule compound modified on the surface of the flexible screen-printed electrode, and the surface of the flexible screen-printed electrode is sealed by bovine serum albumin. The invention further discloses a preparation method adopting a dispensing method. The invention also discloses an application process of dropwise adding a sample solution into the electrochemical biosensor for incubation and detecting electrochemical impedance by adopting an electrochemical workstation. The specific binding capacity of the sensor and folate receptors on the surfaces of tumor cells is high, and rapid detection of the tumor cells is achieved; the preparation method is simple; and according to the application process, the detection efficiency of the tumor cells is improved.

Description

technical field [0001] The invention belongs to the technical field of rapid detection, and in particular relates to an electrochemical biosensor for rapid detection of tumor cells, a preparation method and application thereof. Background technique [0002] Malignant tumors are on the rise all over the world. Malignant tumors are usually discovered late and progress rapidly. Currently, there is still a lack of effective early detection methods. Focusing on the secondary prevention of tumors, the detection of precancerous lesions and early malignant tumors by means of screening is conducive to the implementation of early intervention to reduce morbidity and mortality. Therefore, it is of great significance to carry out rapid and simple screening work. Clinically, the disease is evaluated through laboratory examination of tumor samples obtained by methods such as tissue examination, and various methods have also been proposed in the prior art for capturing and detecting cance...

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Problems solved by technology

[0005] In various reported biosensors based on folic acid functionalization, many nanomaterials have been used to detect cancer cells, such as carbon nanotubes, gold nanoparticles, graphene and dendrimers, etc., but in these methods, folic acid is mostly composed of EDC The acid amine condensation reaction of NHS is covalently immobilized on the electrode surface, the reaction steps are complicated, the reaction time is long, and the reaction conditions are strict

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