Bioreactor system for long-term and large-scale expansion of human umbilical cord mesenchymal stem cells

A kind of stem cell, biological response technology, applied in the field of stem cells, can solve the problem that bone graft is not optimal

Active Publication Date: 2022-01-25
北京国卫生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, static culture is not optimal for growing centimeter-sized bone grafts for clinical translation due to poor nutrient supply and removal of metabolic waste products

Method used

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  • Bioreactor system for long-term and large-scale expansion of human umbilical cord mesenchymal stem cells
  • Bioreactor system for long-term and large-scale expansion of human umbilical cord mesenchymal stem cells

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Embodiment Construction

[0027] The present invention may be understood more readily by reference to this Detailed Summary. The terms used herein are for the purpose of describing particular embodiments only and do not limit the claims, and unless specifically defined herein, terms used herein are to be given their conventional meanings as known in the relevant art.

[0028] In the following description, certain specific details are set forth in order to provide a thorough understanding of various disclosed embodiments. One skilled in the relevant art will recognize, however, that the embodiments may be practiced without one or more of these specific details, or with other methods, components, materials, etc. In other instances, well-known structures associated with computing systems, including client and server computing systems, and networks have not been shown or described in detail to avoid unnecessarily obscuring the description of the embodiments.

[0029] Such as figure 2 As shown, the long-...

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Abstract

The present invention proposes a biological reaction system for long-term and large-scale expansion of human umbilical cord mesenchymal stem cells, including: a culture module formed by a plurality of nanofiber tubes and a substrate; the substrate has a three-dimensional network formed by a plurality of fiber support cloths Innumerable small holes are formed on the substrate composed of multiple fiber support cloths; the bioreactor has an input pipe at the upper end, which is connected to the culture fluid storage, and is used to receive the culture fluid input from the culture fluid storage according to the controlled flow rate and flow rate. There is an output pipe at the lower end of the bioreactor, which periodically discharges the internal culture liquid through a valve; the bioreactor contains a porous carrier for absorbing the culture liquid; the culture liquid storage is used for storing the culture liquid, An oxygen containment space is provided at the bottom thereof; a control system including an electronic logic module and a remote computing device, the electronic logic module being wirelessly communicatively coupled to the remote computing device.

Description

technical field [0001] The invention belongs to the technical field of stem cells, in particular to a biological reaction system for long-term large-scale expansion of human umbilical cord mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (MSCs) refer to a type of adult stem cells with self-renewal and multilineage differentiation potential, mainly derived from mesoderm tissue. Since MSCs were first discovered in bone marrow and successfully isolated and cultured in the 1960s, subsequent researchers have isolated MSCs from fat, placenta, umbilical cord, dental pulp and other tissues. MSCs can differentiate into multiple germ layers and types of cells, such as epithelial cells of the ectoderm; adipocytes, chondrocytes, and bone cells of the mesoderm; insulin-secreting cells of the endoderm. MSCs come from various sources, are easy to isolate and expand, have low immunogenicity, strong renewal and differentiation capabilities, and a wide range of tr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/38C12M1/36C12M1/34C12M1/04
CPCC12M23/20C12M25/10C12M25/14C12M37/04C12M41/26C12M41/32C12M41/34C12M41/36C12M41/38C12M41/48
Inventor 李晓玉陈晖马浩天武威
Owner 北京国卫生物科技有限公司
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