Oxygen-resistant lactobacillus plantarum and application thereof

A lactic acid bacteria, oxygen-resistant technology, applied in the application, bacteria, separation of microorganisms and other directions, can solve the problems of reducing the quality and utilization efficiency of silage, loss of nutrients, slow pH drop, etc., to improve the quality of silage fermentation, easy to use, and effective more effect

Pending Publication Date: 2021-12-03
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing lactic acid bacteria additives are all screened under anaerobic conditions, that is, they perform better in an anaerobic environment, but in the actual production of silage, it is impossible to completely eliminate the air between the raw material particles and the empty stems. Especially in the first few days after the cellar is sealed, it is an environment with a lot of air. The growth and cell viability of lactic acid bacteria are often subjected to the oxygen stress of active oxygen free radicals, which affects the reproduction and acid production capacity of lactic acid bacteria; At the same time, oxygen stress will undoubtedly increase the production cost during the production of lactic acid bacteria preparations
Once the lactic acid production is insufficient and the pH drops slowly during the silage process, it will encourage the activities of harmful microorganisms such as Clostridium difficile, cause excessive loss of nutrients, and reduce the quality and utilization efficiency of silage

Method used

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  • Oxygen-resistant lactobacillus plantarum and application thereof
  • Oxygen-resistant lactobacillus plantarum and application thereof
  • Oxygen-resistant lactobacillus plantarum and application thereof

Examples

Experimental program
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example 1

[0023] Example 1: Isolation, screening and physiological and biochemical determination of lactic acid bacteria

[0024] 266 strains of lactic acid bacteria were isolated from 26 kinds of materials such as elephant grass, Italian ryegrass, alfalfa, stylo, paspalum, silage such as elephant grass, corn, soil, and cow dung, and carried out Gram staining and Observation of cell shape, and according to aerobic culture and growth pH (3.5, 4.0, 4.5, 7.5, 8.0, 8.5) and other experiments to screen out oxygen-resistant, acid-resistant and fast-growing lactic acid bacteria SXC48. The specific steps are as follows: sterilize several Erlenmeyer flasks filled with 500mL distilled water, test tubes filled with 9mL distilled water, MRS solid medium and plate, and then pour the MRS solid medium into the plate after sterilization; under sterile conditions, Take 10g of the sample in a plastic bag, add 90mL of sterilized distilled water and shake evenly to make the final concentration 100g / L, then...

example 2

[0039] Example 2: Identification of Lactic Acid Bacteria

Embodiment 1

[0040] The bacterial strain obtained in Example 1 was cultured overnight at 37°C in 5 mL of MRS medium, the bacterial solution was transferred into a 1.5 mL centrifuge tube, centrifuged at 10,000 rpm for 3 min to 5 min to collect the bacteria, and TE (10 mmol / L Tris-HCl, 0.1 mmol / L EDTA , pH 8.0) solution to wash twice, then extract the full-length gene of lactic acid bacteria SXC48, and then use PCR amplification primers 25F (5'-AACTGAAGAGTTTGATCCTGGCTC-3') and 1492R (5'-TACGGCTACCTTGTTACGACT-3') to amplify 16SrDNA The gene (specifically as follows) was sent to Huada Genomics (China) for sequencing, and related sequences were compared on NCBI, and SXC48 was determined to be Lactobacillus plantarum.

[0041] GCGTCAGTTACAGACCAGACAGCCGCCTTCGCCACTGGTGTTCTTCCATATATCTACGCATTTCACCGCTACACATGGAGTTCCACTGTCCTCTTCTGCACTCAAGTTTCCCAGTTTCCGATGCACTTCTTCGGTTGAGCCGAAGGCTTTCACATCAGACTTAAAAAACCGCCTGCGCTCGCTTTACGCCCAATAAATCCGGACAACGCTTGCCACCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAAATACCG...

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Abstract

The invention discloses oxygen-resistant lactobacillus plantarum and application thereof. The oxygen-resistant lactobacillus plantarum is named as Lactobacillus plantarum SXC48, and is preserved in Guangdong Microbiological Culture Collection Center, the preservation place is Guangdong Institute of Microbiology on 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou, the preservation number is GDMCC No: 61621, and the preservation date is April 25, 2021. The lactobacillus plantarum is a Gram staining positive bacillus, is glucose homofermentation, is acid-resistant, fast in growth speed, wide in growth temperature range and particularly oxygen-resistant, and can obviously improve the fermentation quality of silage which is not easy to compact or easy to remain air.

Description

technical field [0001] The invention belongs to the technical field of microbial application and feed preparation and processing, and in particular relates to an oxygen-tolerant lactic acid bacterium and its application. Background technique [0002] Silage is a forage processing and storage method commonly used at home and abroad, and it is also an effective means for modern grassland animal husbandry to improve production capacity and efficiency. Silage can not only improve the quality of meat and milk, but also make full use of plant materials that cannot be directly fed, and reduce the waste of by-products of agricultural product processing. Therefore, silage has been valued by countries all over the world, especially the developed countries of animal husbandry. Silage relies on lactic acid bacteria attached to the surface of silage raw materials to convert water-soluble carbohydrates in the raw materials into lactic acid-based organic acids under anaerobic conditions, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A23K30/18C12R1/25
CPCC12N1/20C12N1/02A23K30/18Y02P60/87
Inventor 张建国田静尹祥
Owner SOUTH CHINA AGRI UNIV
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