Unlock instant, AI-driven research and patent intelligence for your innovation.

SSR molecular markers for identifying cannabis sativa varieties and application of SSR molecular markers

A technology of molecular markers and marijuana, applied in the field of molecular breeding and identification of crops, to achieve the effect of accurate and reliable results, accurate results and improved efficiency

Pending Publication Date: 2021-12-03
黑龙江省农业科学院农产品质量安全研究所
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there are few reports on the construction of cannabis SSR fingerprints

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SSR molecular markers for identifying cannabis sativa varieties and application of SSR molecular markers
  • SSR molecular markers for identifying cannabis sativa varieties and application of SSR molecular markers
  • SSR molecular markers for identifying cannabis sativa varieties and application of SSR molecular markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 Determination of cannabis SSR molecular markers and primers

[0050] According to 22 cannabis varieties (Yousha-31, Longma No. 1, Longmai No. 2, Huoma No. 1, Qingmai No. 1, Longmai No. 3, Longma No. 1, Hanma No. 2, Hanma No. 1 , Longma No. 2, Grecia, Hanma No. 5, Hanma No. 4, Muma No. 1, Qingma No. 2, Xianma No. 1, Longma No. 4, Qingmai No. 3, Qingmai No. 4, The gene sequences of Hanma No. 6, Hanma No. 7, and Longmai No. 5) were searched for SSR sites, and 300 pairs of SSR primers were designed; Grecia, Huoma No. 1, Longmai No. 5, and Hanma No. 7 were extracted , Longmai No. 1 and Yousha-31 DNA, respectively use these 300 pairs of primers to carry out PCR and polyacrylamide gel electrophoresis for these six varieties, analyze and compare the annealing temperature, whether it can be amplified, and the band of each pair of primers Parameters such as specificity, clarity, and whether a stable single band can be obtained in the six varieties, and 210 pairs of ...

Embodiment 2

[0053] Embodiment 2 Utilizes the SSR molecular marker provided by the present invention to distinguish cannabis varieties

[0054] (1) Rapid extraction of DNA

[0055] DNA extraction from hemp seeds was carried out by CTAB method. This method is quick and easy to operate, and the quality of the extracted DNA is high. It is suitable for DNA preparation in the field of plant molecular biology, and it is of great significance for greatly shortening the time of seed purity inspection and transgenic detection, improving detection efficiency, and reducing detection costs. The specific operation steps are as follows: take a few hemp seeds and grind them into powder, put them in a 1.5mL centrifuge tube; add 700 μL of CTAB extract to the centrifuge tube, and incubate at 65°C for 30 minutes; add 500 μL of chloroform-isoamyl alcohol ( 24:1), shake vigorously, centrifuge at 12000rpm for 10min; take the supernatant, add 0.7 times the volume of isopropanol, and centrifuge at 12000rpm for 5...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides SSR molecular markers for identifying cannabis sativa varieties and an application of the SSR molecular markers. The SSR molecular markers for identifying the cannabis sativa varieties comprise 11 SSR molecular markers, and based on the 11 SSR molecular markers, the invention further provides a primer combination which is suitable for a capillary electrophoresis platform and is used for detecting the SSR molecular markers. The 11 SSR molecular markers can be used for identifying the variety of cannabis sativa and analyzing genetic diversity. The varieties of Geliexiya cannabis sativa and hemp No.1, Long cannabis sativa No.5, China hemp No.7, and Long cannabis sativa No.1 and Yousha-31 are distinguished. Through the application of the cannabis sativa SSR molecular markers, the range of available marker sites of the cannabis sativa can be expanded on the genome level. A new tool is provided for researches on the varieties of the cannabis sativa, germplasm resource identification, genetic relationship evaluation, cytoplasm hereditary characteristics and the like, and the application prospect is good.

Description

technical field [0001] The invention relates to the technical field of crop molecular breeding and identification, in particular to a group of SSR molecular markers for identifying cannabis varieties and applications thereof. Background technique [0002] Cannabis (Cannabis sativa L.) is an annual herb belonging to the Cannabis family Cannabis family. There are three varieties under it which are industrial hemp, Indian hemp and wild hemp. Industrial hemp is the most common type, referring to hemp varieties with a tetrahydrocannabinol content of less than 0.3%, which has a high economic value. Because hemp seed oil contains balanced omega-3 and omega-6 unsaturated fatty acids, it is mostly used in food, cosmetics and medicines; cannabidiol (CBD, cannabidiol) in hemp belongs to terpene phenolic compounds and has rich Pharmacological effects; hemp fiber and stalk core are excellent raw materials for building materials, special military supplies, baby underwear, textiles, etc....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2600/13C12Q2531/113C12Q2565/125C12Q2563/107
Inventor 关海涛张晓磊陈中华张瑞英尹阳兰青阔
Owner 黑龙江省农业科学院农产品质量安全研究所