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Glutathione lightening type non-sulfydryl gold nano-material as well as preparation method and application thereof

A technology of glutathione and nanomaterials, applied in nanotechnology for sensing, analytical materials, nanotechnology, etc., can solve problems such as poor stability of optical properties, short emission wavelength of dyes, poor biocompatibility, etc. Achieve the effect of low cost, wide emission wavelength range and low toxicity

Active Publication Date: 2021-12-07
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are currently many reports on the use of small molecule dyes to analyze the glutathione content in organisms through fluorescence detection methods (J HazardMater, 2021, 413, 125332), but in the process of analysis and detection, small dye molecules are easy to photobleach, and optical Poor property stability, more hydrophobic parts in the structure lead to poor biocompatibility, and the short emission wavelength of the dye will be interfered by the background fluorescence of the protein in the organism

Method used

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  • Glutathione lightening type non-sulfydryl gold nano-material as well as preparation method and application thereof
  • Glutathione lightening type non-sulfydryl gold nano-material as well as preparation method and application thereof
  • Glutathione lightening type non-sulfydryl gold nano-material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] The preparation steps of glutathione-lighted non-thiol-based nanomaterials are as follows:

[0072] At room temperature, add 28.5 μL of chloroauric acid aqueous solution (0.355M) to a 50 mL round bottom flask filled with 20.8 mL of deionized water, followed by 1.8 mL of triphenylphosphine triisosulfonic acid sodium salt solution (10 mM) , stirred at 500rpm / min at room temperature for 30min, and when the solution changed from light yellow to colorless and transparent, 2.4mL of sodium borohydride aqueous solution (25mM) was added, and the solution immediately turned into brownish yellow. After stirring at room temperature for 12h, the reaction was stopped, and then in Centrifuge at 4°C and 21,000rpm for 1 hour to remove large particles, and then use a 10kDa ultrafiltration tube to remove unreacted substrates by ultrafiltration at 4°C and 4,000rpm for 10 minutes and concentrate to obtain the target product, which is stored in a 4°C refrigerator for later use.

[0073] The ...

Embodiment 2

[0087]Example 1 The implementation of glutathione-lighted non-thiol-based nanomaterials for glutathione detection is as follows: 25.8 μL of nanomaterials (15.47 mM) prepared above, 250 μL of glutathione solution (1.0 mM, in 1x PB buffer), 13.7 μL of sodium chloride (5M) and 210.5 μL of deionized water were mixed, the pH was about 7.4, incubated at 37°C for 3-24h on a constant temperature incubator, and then tested with a fluorometer. Sulfhydryl-based nanomaterials are lit up by glutathione.

[0088] Figure 9a It is a change diagram of the 400-900nm fluorescence spectrum illuminated by glutathione of non-mercapto-based nanomaterials with the incubation time prolonging.

[0089] Figure 9b It is a change diagram of the 900-1200nm fluorescence spectrum illuminated by glutathione of non-mercapto-based nanomaterials with the incubation time prolonging.

[0090] from Figure 9a with Figure 9b It can be seen that the fluorescence of non-thiol-based nanomaterials lit by glutath...

Embodiment 3

[0092] Example 1 Preparation of glutathione-lighted non-thiol-based nanomaterials for glutathione detection is as follows: 25.8 μL of nanomaterials (15.47 mM) prepared above, 250 μL of different concentrations of glutathione solutions (in 1x PB buffer), 13.7 μL of sodium chloride (5M) and 210.5 μL of deionized water were mixed, the pH was about 7.4, incubated on a constant temperature incubator at 37°C for 24 hours, and then tested for non-mercapto-based nanoparticles with a fluorometer The material is lightened by the glutathione effect.

[0093] Figure 10 a is the change of fluorescence intensity at 800nm ​​after the non-thiol-based nanomaterials were incubated with different concentrations of glutathione. When the concentration of glutathione was 0.5mM, the signal-to-noise ratio was as high as 25.

[0094] Figure 10 a is the change of fluorescence intensity at 1026nm after the non-mercapto-based nanomaterials were incubated with different concentrations of glutathione. ...

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Abstract

The invention discloses a glutathione lightening type non-sulfydryl gold nano-material as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out a stirring reaction on chloroauric acid and a non-sulfydryl ligand in a solvent; and adding a reducing agent, performing a stirring reaction, and purifying to obtain the glutathione lightening type non-sulfydryl gold nano-material. The non-luminous non-sulfydryl gold nano-material is applied to detection of glutathione, and compared with a common glutathione detection reagent, the non-luminous non-sulfydryl gold nano-material has lower background interference and better biocompatibility. The non-luminous non-sulfydryl gold nano-material is simple in preparation method, low in cost and easy for industrial production, and due to the lightening performance of glutathione, the non-luminous non-sulfydryl gold nano-material also has a very good effect when being used for detecting glutathione in cells and tumors.

Description

technical field [0001] The invention belongs to the field of functional optical nanometer materials, and in particular relates to a glutathione-lighted non-thiol-based nanometer material and its preparation method and application. Background technique [0002] Glutathione is a natural tripeptide consisting of glutamic acid, cysteine ​​and glycine. As the most abundant non-protein thiol compound synthesized in the cytosol, glutathione has multiple roles in regulating cellular physiological functions, including: (1) direct scavenging of reactive oxygen species, nitric oxide and their derivatives , thus protecting the electron transport chain, DNA, lipids and proteins; (2) detoxification of endogenous compounds and exogenous organisms; (3) S-glutathione sulfonylation of protein thiol groups; (4 ) regulation of cell cycle progression and apoptosis. So, glutathione is not just a free radical scavenger, it also plays an important role in the control of cell survival, necrosis an...

Claims

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Application Information

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IPC IPC(8): B22F9/24A61K49/00B22F1/00B82Y15/00B82Y40/00G01N21/64G01N27/626
CPCB22F9/24B82Y40/00B82Y15/00A61K49/0065G01N21/6428G01N21/6486G01N27/626
Inventor 刘锦斌陈华瑞
Owner SOUTH CHINA UNIV OF TECH
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