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Method for improving beef production performance of beef cattle by using TTN gene

A beef cattle and gene technology, applied in the field of molecular biology, can solve the problems of poor fat deposition, slow growth rate of Qinchuan cattle, and low meat production, and achieve the effect of improving meat production performance.

Pending Publication Date: 2021-12-07
INNER MONGOLIA UNIVERSITY +3
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, compared with foreign high-quality beef cattle breeds, Qinchuan cattle have problems such as slow growth, low meat production and poor fat deposition.

Method used

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  • Method for improving beef production performance of beef cattle by using TTN gene
  • Method for improving beef production performance of beef cattle by using TTN gene
  • Method for improving beef production performance of beef cattle by using TTN gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Obtaining SNP Detection Fragments of Qinchuan Cattle and Chinese Simmental Cattle TTN Gene and Establishment of Polymorphism Site Detection Method

[0031] 1. Extraction of Genomic DNA from Qinchuan Cattle and Chinese Simmental Cattle

[0032] The test cattle of the present invention are 350 Qinchuan adult cows (age 18-24 months old, at least three generations unrelated) of the National Beef Cattle Improvement Center of Northwest Agriculture and Forestry University, and 260 Chinese Ximen cows of Chifeng Shengquan Ecological Animal Husbandry Co., Ltd. Tahr cattle (age 14-18 months). The genomic DNA of Qinchuan cattle and Chinese Simmental cattle was extracted using the blood genomic DNA extraction kit produced by Beijing Tiangen Biochemical Technology Co., Ltd., and the specific steps refer to the kit instructions. Evaluate the quality and quantity of the extracted DNA, evaluate it by agarose gel electrophoresis, and store it at -40°C for future use.

[0033]...

Embodiment 2

[0047] Example 2 Detection of polymorphic distribution of molecular markers in Qinchuan cattle, Chinese Simmental cattle and other local beef cattle breeds

[0048] The genetic diversity of the TTN gene g.18060564C>T locus was analyzed in 7 cattle populations, and the results are shown in Table 3.

[0049] Table 3 Genotype frequency and allele frequency of TTN gene g.18060564C>T site in cattle population

[0050]

[0051] Note: QC, Qinchuan cattle; LX, Luxi cattle; MGC, Mongolian cattle group (Inner Mongolia Autonomous Region, China); MGG, Mongolian cattle group (Mongolia); WL, Wuling cattle; LL, Longlin cattle; CS, China Simmental.

[0052] From the results in Table 3, it can be known that the TTN gene g.18060564C>T site has three genotypes in 7 cattle populations: TT, TC and CC; the genotype frequency of the TTN gene g.18060564C>T site in Chinese Qinchuan cattle is : TT(0.017), TC(0.249), CC(0.734), C allele frequency (0.859) is higher than T allele frequency (0.141); C...

Embodiment 3

[0053] Example 3 Correlation Analysis and Application of Molecular Markers and Meat Performance of Qinchuan Cattle and Chinese Simmental Cattle

[0054] The correlation analysis between Qinchuan cattle and Chinese Simmental cattle TTN gene g.18060564C>T loci and meat production performance was analyzed using SPSS 19.0, and the general linear model was constructed as follows:

[0055] Y ijklm =μ+G i +A j +F k +S l +S m +e ijklm ;

[0056] Among them, Yijklm is the observed value of the trait, μ is the average value of the trait, G i is the genotype effect, A j is the fixed effect caused by age, F k is the pasture environmental effect, S l is the gender effect, S m is the family effect, e ijklm is a random error.

[0057] In Qinchuan cattle, the correlation analysis of TTN gene g.18060564C>T locus and meat production performance, TT genotype individuals are less than 10, no statistics, CC and CT genotype statistical analysis results are shown in Table 4 and Table 5 ...

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Abstract

The invention belongs to the technical field of molecular biology, and particularly relates to a method for improving the beef production performance of a beef cattle by using a TTN gene g.18060564C > T site. The method for improving the beef production performance of the beef cattle by using the TTN gene g.18060564C > T site comprises the following steps of: detecting whether mutation of the g.18060564C > T site exists in the TTN gene in a beef cattle genome to be detected; and selecting a beef cattle individual with the genotype of CT type or CC type to carry out artificial insemination and natural mating. The above molecular marker is used as a reliable marker for the beef production performance of the Qinchuan cattle and the Chinese Simmental cattle, a technical system for detecting the genotype through DNA is established, so that selection intensity and breeding accuracy and efficiency are improved, and the beef production performance of the Qinchuan cattle and the Chinese Simmental cattle is improved.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a method for improving the meat production performance of beef cattle by utilizing the g.18060564C>T site of the TTN gene. Background technique [0002] Qinchuan cattle are named after the "Eight Hundred Li Qinchuan" located in Guanzhong, Shaanxi. Appropriate pattern and other characteristics. Qinchuan cattle breeding has become a pillar industry for farmers in Shaanxi, Gansu, Ningxia and other provinces to increase their income. However, compared with foreign high-quality beef cattle breeds, Qinchuan cattle have problems such as slow growth, low meat production and poor fat deposition. [0003] Simmental cattle are native to Switzerland. In 1990, the cattle and sheep breeding base of Shandong Provincial Animal Husbandry Bureau introduced this breed. Simmental cattle are not purebred beef cattle, but due to their high milk production, the meat producti...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 佟彬澈力木格成功昝林森青格乐图殷志成
Owner INNER MONGOLIA UNIVERSITY
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