Organoid culture chip and organoid culture method

Abstract

The invention discloses an organoid culture chip and culture method. The organoid culture chip comprises a cell culture plate; and an organoid culture device arranged in the cell culture plate. A culture medium liquid storage tank is formed between the organoid culture device and the cell culture plate; and the organoid culture device comprises an organoid culture device body, an organoid culture chamber arranged in the organoid culture device body and side holes formed in side walls of the two sides of the organoid culture device body, the organoid culture chamber communicates with the side holes in the side walls of the two sides to form perfusion channels, the organoid culture chamber comprises a sample adding hole formed in the top and a micropore formed in the bottom, and both the sample adding hole and the micropore communicate with the bottom of the cell culture plate. According to the organoid culture chip and the culture method, high-throughput and one-step dynamic perfusion culture of organoids with a uniform form can be realized, and the whole growth and development process of the organoids can be dynamically observed in situ.

Description

technical field [0001] The invention relates to the technical fields of tissue engineering and organ chips, in particular to an organoid culture chip and an organoid culture method. Background technique [0002] In recent years, with the development of cell biology and tissue engineering, three-dimensional cell models are gradually replacing traditional two-dimensional cell models. As a new type of three-dimensional in vitro research model, organoids are self-assembled by stem cells in vitro and grow into three-dimensional aggregates similar to human tissues or organs in structure and function, such as: brain organoids, blood vessel organoids, liver organs, kidney organoids and tumor organoids, etc. On a global scale, organoids have shown their strong development potential, and a certain competitive pattern has been formed internationally, but our country is still in its infancy. In addition, the "Technical Guidelines for Non-clinical Research and Evaluation of Gene-Modifi...

AI Technical Summary

Problems solved by technology

However, during organoid transfer, the outer layer of organoid cells is vulnerable to mechanical damage and increases the chance of contamination

In addition, in the process of suspension culture, the phenomenon of mutual fusion between organoids is easy to occur, and it is difficult to locate and observe.

The above limitations lead to the limitations of the organoid culture process, such as complexity, great variability, low throughput, and difficulty in real-time monitoring.

[0004] Therefore, in order to overcome the technical problems of low throughput and complexity of the above-mentioned traditional organoid culture methods, it is necessary to develop a high-throughput in situ culture organoid culture chip for the application research of organoids.

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