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Kit for detecting in-vitro antioxidant activity of xylaria intracellular polysaccharide and detection method thereof

A technology for antioxidant activity and detection kits, applied in color/spectral characteristic measurement, measurement devices, material analysis through optical means, etc., can solve problems such as sample contamination, affecting detection accuracy, complicated operation, etc., and achieve the detection process Effects of simplification, reduction of contamination probability, and improvement of reliability

Active Publication Date: 2021-12-10
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a test kit for the in vitro antioxidant activity of the intracellular polysaccharide of Xyrokeratum and its detection method, which solves the complex operation and easy Cause sample contamination, affect detection accuracy and other defects

Method used

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  • Kit for detecting in-vitro antioxidant activity of xylaria intracellular polysaccharide and detection method thereof
  • Kit for detecting in-vitro antioxidant activity of xylaria intracellular polysaccharide and detection method thereof
  • Kit for detecting in-vitro antioxidant activity of xylaria intracellular polysaccharide and detection method thereof

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Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1: the setting of detection kit

[0032] combine Figure 1 to Figure 4 As shown, a test kit for the in vitro antioxidant activity of the intracellular polysaccharide of Xyrokeratum, including a detection reagent and a reaction plate, the detection reagent includes an extraction solvent for the intracellular polysaccharide of Xyrokeratum and a detection reagent for the antioxidant activity of the polysaccharide, and the reaction plate consists of the following: Bottom layer 1, quantitative layer 2 and grinding layer 3 stacked upwards in sequence;

[0033] Several grooves 12 for fixing the cuvette 11 are formed on the bottom layer 1, preferably, the shape of the groove 12 matches the shape of the cuvette 11, and the depth of the groove 12 exceeds half of the height of the cuvette 11 One, easy to fix;

[0034] Quantitative layer 2 is provided with a plurality of quantitative cups 21, and each quantitative cup 21 has a drop hole 22 at the bottom, and the drop h...

Embodiment 2

[0047] Example 2: In vitro antioxidant activity detection of intracellular polysaccharides

[0048] (1) Preparation of Anthracite

[0049] (1) Source of strain: obtained by isolation after commercial purchase.

[0050] (2) Strain medium: (2.1) Plate medium: comprehensive PDA, natural pH. (2.2) Liquid medium: glucose 2%, peptone 1%, pH natural. (2.3) Synthetic Medium (g) A: MnSO 4 4H 2 O 0.001g, FeSO 4 ·7H 2 O0.005g, ZnSO 4 ·7H 2 O 0.01g, NaCl 0.25g, CuSO 4 4H 2 0 0.01g, NaHPO 4 1.00g, glucose 30g, aspartic acid 4.00g, distilled water to 1000mL. B: CaCO 3 0.25g, NaCl 0.20g, MgSO 4 0.20g, KHPO 4 1.00g, Na 2 HPO 4 1.00g, glucose 30g, aspartic acid 4.00g, dissolved in distilled water to 1000mL. All the above media were sterilized at 15 pounds for 30 minutes before use.

[0051] (3) Fermentation culture: 150mL of liquid culture medium in 250mL triangular flask, use a hole puncher with a diameter of 1cm to punch holes on the plate medium covered with mycelia, i...

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Abstract

The invention discloses a kit for detecting the in-vitro antioxidant activity of xylaria intracellular polysaccharide and a detection method thereof. The kit comprises a detection reagent and a reaction plate, and the reaction plate comprises a bottom layer, a quantitative layer and a grinding layer which are sequentially stacked from bottom to top; a plurality of grooves for fixing cuvettes are formed in the bottom layer; a plurality of quantitative cups are arranged on the quantitative layer in a penetrating manner, and a dripping hole is formed in the bottom of each quantitative cup; a grinding cavity is formed in the grinding layer, a grinding mechanism is arranged in the grinding cavity, and a plurality of filtering holes are formed in the bottom of the grinding cavity. The kit has the functions of grinding wall breaking, extraction, filtration, quantification, standing and the like, extraction of xylaria intracellular polysaccharide can be realized only by adopting the kit, in-vitro antioxidant activity detection can be performed by matching with a spectrophotometer, the whole detection process is greatly simplified, the pollution probability of a sample solution is reduced, the reliability of a detection result is improved, and an important guarantee is provided for the research on the health-care efficacy of xylaria and related medicines.

Description

technical field [0001] The invention relates to the technical field of fungal detection, in particular to an in vitro antioxidative activity detection kit and detection method of the intracellular polysaccharide of Ceratocystis cerevisiae. Background technique [0002] Fungal polysaccharides are high molecular polymers isolated from fungi that are composed of more than 10 monosaccharides connected by glycosidic bonds and are insoluble in organic solvents such as high-concentration ethanol, n-butanol, and acetone. Metabolites isolated from the body and fermentation broth. A large number of pharmacological experiments have shown that fungal polysaccharides can eliminate free radicals in the body and have antioxidant properties, so they have activities such as immune regulation, anti-tumor, lowering glycolipids, and delaying aging, and have been widely used in the field of medical care.” So far, More than 300 kinds of polysaccharides have been isolated from natural products, a...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/01
CPCG01N21/31G01N21/01
Inventor 赵方熙王志花韩东晶刘国庆
Owner HEFEI UNIV OF TECH
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