Saxifraga stolonifera extract with anti-inflammatory activity as well as preparation method and application thereof

An extraction method, the technology of saxifrage, is applied in the direction of medical preparations containing active ingredients, anti-inflammatory agents, drug combinations, etc., which can solve the problems of different drug effects, storage and preservation of fresh saxifrage, high processing and transportation costs, and inability to clarify Material basis and other issues to achieve the effect of reducing inflammatory factors

Inactive Publication Date: 2021-12-14
BEIJING UNIV OF AGRI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies in recent decades have shown that traditional Chinese medicines that have a good killing effect on bacteria screened in vitro cannot exert their antibacterial activity in vivo, but some traditional Chinese medicines that have no bactericidal activity in vitro show good antibacterial activity in vivo. The bactericidal effect is likely to be due to the inhibition of the expression of pro-inflammatory factors by downgrading the toxins secreted by pathogenic microorganisms in traditional Chinese medicine to achieve the bactericidal effect
[0004] The active ingredients of saxifrage are mainly flavonoids, terpenoids, phenolic acids and coumarins. The difference in active ingredients between fresh grass and dry grass leads to different efficacy. Stable, its material basis cannot be clarified, and due to the high cost of storage and preservation of fresh grass, processing and transportation, the development and utilization of saxifrage resources are limited

Method used

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  • Saxifraga stolonifera extract with anti-inflammatory activity as well as preparation method and application thereof
  • Saxifraga stolonifera extract with anti-inflammatory activity as well as preparation method and application thereof
  • Saxifraga stolonifera extract with anti-inflammatory activity as well as preparation method and application thereof

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preparation example Construction

[0032] The invention provides a preparation method of saxifrage extract with anti-inflammatory activity, such as figure 1 As shown, the preparation method includes

[0033] S1: using different extraction methods to extract fresh saxifrage grass and dry saxifrage grass to obtain extracts;

[0034] S2: comparing the content differences of total flavonoids and total phenolic acids in the fresh saxifrage extract and dry saxifrage extract obtained by different extraction methods;

[0035] S3: Qualitative analysis of the active components in the fresh saxifrage extract and the dry saxifrage extract;

[0036] S4: Establishing an inflammation model and adding the interacting fresh saxifrage extract and dried saxifrage extract to verify the effects of the fresh saxifrage extract and dried saxifrage extract.

[0037] In the above preparation method, the specific method of the extraction method is not limited, but in order to further understand the influence of each extraction method o...

Embodiment 1

[0065] Preparation of decocted saxifrage fresh herb extract / water decocted saxifrage dried herb extract:

[0066] Take 50g of dried saxifrage or 378g of fresh saxifrage (equivalent to 40g of dried saxifrage), add distilled water until the saxifrage is submerged, soak for 30min, boil over strong fire and turn to slow fire for 30min, filter with triple gauze filtrate. Among them, the temperature of strong fire is 180°C, and the temperature of slow fire is 80°C; for dry saxifrage, the amount of water is 700mL; for fresh saxifrage, the amount of water is 2000mL.

[0067] Repeat the above steps three times, combine the filtrates, evaporate and concentrate to a crude drug concentration of 1 g / mL, and dry to constant weight to obtain saxifrage hay and fresh herb extracts, which are dried and then gently scraped off.

[0068] Saxifraga decocted fresh grass and decocted hay were dried to constant weight, and scraped to find that the decocted fresh grass had two distinct layers, the up...

Embodiment 2

[0070] Preparation of fresh saxifrage extract by water extraction: Take 50 g of dry saxifrage powder, add 600 mL of distilled water, ultrasonicate at 30°C for 1 hour (ultrasonic frequency is 40KHz), and filter. Repeat the above steps three times, combine the filtrates, evaporate and concentrate to a crude drug concentration of 1 g / mL, and dry to constant weight to obtain saxifrage hay and fresh herb extracts, which are dried and then gently scraped off.

[0071] Preparation of water-extracted saxifrage hay extract: wash and drain the fresh saxifrage, accurately weigh 473g, pulverize, add water 5676mL, ultrasonicate at 30°C for 1h (ultrasonic frequency is 40KHz), and filter. Repeat the above steps three times, combine the filtrates, evaporate and concentrate to a crude drug concentration of 1 g / mL, and dry to constant weight to obtain saxifrage hay and fresh herb extracts, which are dried and then gently scraped off.

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Abstract

The invention discloses a saxifraga stolonifera extract with anti-inflammatory activity as well as a preparation method and application thereof, the preparation method comprises the following steps: S1, extracting fresh saxifraga stolonifera and hay saxifraga stolonifera by adopting different extraction methods to obtain an extract; S2, comparing the content differences of total flavonoids and total phenolic acids in the fresh saxifraga stolonifera extract and the dry saxifraga stolonifera extract obtained by adopting different extraction methods; S3, performing qualitative analysis on active ingredients in the fresh saxifraga stolonifera extract and the hay saxifraga stolonifera extract; and S4, establishing an inflammation model, and respectively adding the fresh saxifraga stolonifera extract and the hay saxifraga stolonifera extract which interact with each other to verify the effects of the fresh saxifraga stolonifera extract and the hay saxifraga stolonifera extract. The method solves the technical problem that the use of fresh grass and hay of saxifraga stolonifera is limited in the prior art.

Description

technical field [0001] The invention relates to the field of saxifrage extraction, in particular to a saxifrage extract with anti-inflammatory activity and its preparation method and application. Background technique [0002] After invasive bacterial pathogens invade the cells, they release a large number of virulence factors, which promote the increase of cell inflammatory factors and finally invade the cells. In the past, the treatment of bacterial infections was mainly the use of antibiotics. In fact, the recent prohibition of antibiotics has brought new ideas of antibacterial and anti-inflammatory to traditional Chinese medicine. [0003] As a natural product, Chinese herbal medicine has increasingly obvious advantages of multiple functions, low toxicity, no drug resistance, no drug residue, and environmental protection. In addition, about 23% of Chinese herbal medicine is mainly used in fresh medicine. Studies in recent decades have shown that traditional Chinese medic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P29/00
CPCA61K36/185A61P29/00A61K2236/331
Inventor 董虹刘晓晔郭大伟张慧于非可
Owner BEIJING UNIV OF AGRI
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