Pseudomonas Y1 with efficient heterotrophic nitrification and aerobic denitrification functions and embedded pellets and application thereof
A technology of aerobic denitrification and heterotrophic nitrification, applied in Pseudomonas Y1 and its embedded pellets and application fields, can solve the problem of low chemical oxygen demand and nitrogen content, low nitrogen degradation efficiency, and difficulty in forming dominant bacteria kinds of problems
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Embodiment 1
[0045] A kind of Pseudomonas Y1 with efficient heterotrophic nitrification and aerobic denitrification function in this implementation is classified as Pseudomonas sp.Y1 (Pseudomonas sp.Y1), and the preservation unit is: China Typical Culture Collection Center, preservation place It is Wuhan University of China, date of deposit: September 1, 2021, deposit number: CCTCC NO: M20211119.
[0046] Strain screening: Take 10g of activated sludge and inoculate it into a 250mL Erlenmeyer flask filled with 100mL of sterilized distilled water, add some glass beads to the Erlenmeyer flask to break up the mud sample, and then place it on a shaker at 30°C and 170rpm for 20min. Finally, the intermittent aeration method is used to enrich the microorganisms. The frequent intermittent aeration method can eliminate complete aerobic bacteria and anaerobic bacteria, making aerobic denitrifying bacteria the dominant bacterial group. Afterwards, 2 mL of the liquid was added to the sterilized heterot...
Embodiment 2
[0059] The preparation method of Pseudomonas Y1-embedded pellets with efficient heterotrophic nitrification and aerobic denitrification function in this embodiment comprises the following steps:
[0060] 1) Pseudomonas Y1 with efficient heterotrophic nitrification and aerobic denitrification function was inoculated into the heterotrophic nitrification and aerobic denitrification medium, and the formula of the medium was 0.24 g of ammonium sulfate, 4.052 g of sodium succinate, Dipotassium hydrogen phosphate 0.25g, sodium chloride 0.125g, magnesium sulfate heptahydrate 0.125g, manganese sulfate tetrahydrate 0.0025g, at 24-32°C, shaker speed 130-160rpm shaking culture for 48-72h.
[0061] 2) Subsequently, the concentration of ammonium sulfate and sodium succinate in the culture solution was decreased by 20% of the initial concentration every 3 days, until the 15th day when the nutrient concentration was only about 20% of the initial concentration, the nutrient concentration was no...
Embodiment 3
[0065] Such as Figure 1-Figure 3As shown, an advanced treatment system for urban eutrophication landscape water denitrification based on Pseudomonas Y1 embedded pellets with efficient heterotrophic nitrification and aerobic denitrification function, including artificial wetland reaction pool 1 and drop water filling The oxygen device 2, the depth of the constructed wetland reaction pool body 1 is 0.5-0.6m, and the dissolved oxygen concentration in the constructed wetland reaction pool body 1 is ≥8mg / L. The bottom of the artificial wetland reaction tank body 1 is paved with gravel filler 3, the particle size of the gravel filler 3 is 0.15-0.20mm, and the thickness of the gravel filler 3 is 120mm. An ecological floating bed 4 is arranged above the constructed wetland reaction pool body 1; a water inlet pipe 5 is arranged under one side of the constructed wetland reaction pond body 1, and a water outlet pipe 6 is arranged above the other side of the constructed wetland reaction ...
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