Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Pseudomonas Y1 with efficient heterotrophic nitrification and aerobic denitrification functions and embedded pellets and application thereof

A technology of aerobic denitrification and heterotrophic nitrification, applied in Pseudomonas Y1 and its embedded pellets and application fields, can solve the problem of low chemical oxygen demand and nitrogen content, low nitrogen degradation efficiency, and difficulty in forming dominant bacteria kinds of problems

Pending Publication Date: 2021-12-17
中交和美环境生态建设有限公司
View PDF18 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are obvious defects in the biological treatment process at this stage. For example, the nitrification and denitrification in the traditional denitrification process need to be carried out in an aerobic and anoxic environment respectively. Different oxygen environments; in addition, the chemical oxygen demand and nitrogen content in urban eutrophic landscape water bodies are low, and it is difficult for microorganisms to obtain sufficient nutrition to survive; at the same time, the area of ​​urban eutrophic landscape water bodies is large, and microorganisms are easy to lose in water bodies, and it is difficult to The formation of dominant bacterial species leads to low nitrogen degradation efficiency in water bodies; therefore, there is a need for a cost-effective microbial and systematic method that can purify nitrogen in urban eutrophic landscape water bodies using biointensified processes under the same oxygen environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pseudomonas Y1 with efficient heterotrophic nitrification and aerobic denitrification functions and embedded pellets and application thereof
  • Pseudomonas Y1 with efficient heterotrophic nitrification and aerobic denitrification functions and embedded pellets and application thereof
  • Pseudomonas Y1 with efficient heterotrophic nitrification and aerobic denitrification functions and embedded pellets and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A kind of Pseudomonas Y1 with efficient heterotrophic nitrification and aerobic denitrification function in this implementation is classified as Pseudomonas sp.Y1 (Pseudomonas sp.Y1), and the preservation unit is: China Typical Culture Collection Center, preservation place It is Wuhan University of China, date of deposit: September 1, 2021, deposit number: CCTCC NO: M20211119.

[0046] Strain screening: Take 10g of activated sludge and inoculate it into a 250mL Erlenmeyer flask filled with 100mL of sterilized distilled water, add some glass beads to the Erlenmeyer flask to break up the mud sample, and then place it on a shaker at 30°C and 170rpm for 20min. Finally, the intermittent aeration method is used to enrich the microorganisms. The frequent intermittent aeration method can eliminate complete aerobic bacteria and anaerobic bacteria, making aerobic denitrifying bacteria the dominant bacterial group. Afterwards, 2 mL of the liquid was added to the sterilized heterot...

Embodiment 2

[0059] The preparation method of Pseudomonas Y1-embedded pellets with efficient heterotrophic nitrification and aerobic denitrification function in this embodiment comprises the following steps:

[0060] 1) Pseudomonas Y1 with efficient heterotrophic nitrification and aerobic denitrification function was inoculated into the heterotrophic nitrification and aerobic denitrification medium, and the formula of the medium was 0.24 g of ammonium sulfate, 4.052 g of sodium succinate, Dipotassium hydrogen phosphate 0.25g, sodium chloride 0.125g, magnesium sulfate heptahydrate 0.125g, manganese sulfate tetrahydrate 0.0025g, at 24-32°C, shaker speed 130-160rpm shaking culture for 48-72h.

[0061] 2) Subsequently, the concentration of ammonium sulfate and sodium succinate in the culture solution was decreased by 20% of the initial concentration every 3 days, until the 15th day when the nutrient concentration was only about 20% of the initial concentration, the nutrient concentration was no...

Embodiment 3

[0065] Such as Figure 1-Figure 3As shown, an advanced treatment system for urban eutrophication landscape water denitrification based on Pseudomonas Y1 embedded pellets with efficient heterotrophic nitrification and aerobic denitrification function, including artificial wetland reaction pool 1 and drop water filling The oxygen device 2, the depth of the constructed wetland reaction pool body 1 is 0.5-0.6m, and the dissolved oxygen concentration in the constructed wetland reaction pool body 1 is ≥8mg / L. The bottom of the artificial wetland reaction tank body 1 is paved with gravel filler 3, the particle size of the gravel filler 3 is 0.15-0.20mm, and the thickness of the gravel filler 3 is 120mm. An ecological floating bed 4 is arranged above the constructed wetland reaction pool body 1; a water inlet pipe 5 is arranged under one side of the constructed wetland reaction pond body 1, and a water outlet pipe 6 is arranged above the other side of the constructed wetland reaction ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention discloses pseudomonas Y1 with efficient heterotrophic nitrification and aerobic denitrification functions and embedded pellets and application thereof. The pseudomonas Y1 with the efficient heterotrophic nitrification and aerobic denitrification functions is classified and named as pseudomonas Y1, the preservation unit is China Center for Type Culture Collection, the preservation place is Wuhan University, China, the preservation date is September 01, 2021, and the preservation number is CCTCC NO: M20211119. According to the invention, the pseudomonas Y1 screened from activated sludge of an aeration tank of a sewage plant is subjected to hypotrophic domestication and embedding immobilization, and is combined with emergent aquatic plants, so that a hypotrophic micro-polluted water body with low chemical oxygen demand can be effectively treated, as microorganisms are embedded and immobilized, no residual sludge is generated, and the treatment cost is greatly reduced. According to the invention, nitrogen in the landscape water body can be removed under the conditions of low chemical oxygen demand and no use of a physical and chemical method and activated sludge, and the standard of surface water IV type and above water bodies is met.

Description

technical field [0001] The invention relates to the technical field of denitrification and purification, in particular to a Pseudomonas Y1 with efficient heterotrophic nitrification and aerobic denitrification function, its embedded pellets and its application. Background technique [0002] Although the eutrophic landscape water bodies in many cities in my country have far exceeded the standard of the fifth category of surface water, their TN and COD contents are still lower than those of domestic sewage, so the treatment methods are very limited. At present, the treatment methods of urban eutrophic landscape water body include physical method, physical and chemical method, biochemical method and bioremediation method. The main principle of the purification process of physical method is transfer and dilution, and the structure and properties of nitrogen in the water body have not changed; Treatment methods include coagulation and air flotation, etc.; biochemical treatment ma...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/36C12N11/10C12N11/084C12N11/04C02F3/02C02F3/32C02F3/34C12R1/38
CPCC12N1/36C12N1/20C12N11/10C12N11/084C12N11/04C02F3/02C02F3/32C02F3/34Y02W10/10
Inventor 周喜龙周海燕杨俊卢明明王祖辉李昂黎新钢胡鑫王越印一鑫
Owner 中交和美环境生态建设有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products