Analysis device and analysis method
An analysis device and luminous point technology, which is applied in analysis materials, measurement devices, material excitation analysis, etc., can solve problems such as inability to uniquely obtain solutions, and achieve the effect of avoiding the reduction of detection sensitivity, reducing spatial crosstalk and spectral crosstalk.
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Embodiment 1
[0114] To investigate the properties of spatial crosstalk in detail, a figure 1 The simple optical system shown. figure 1 The optical system includes a pinhole plate 1-1, a light-emitting point side opening plate 1-2, a condenser lens 1-3, a sensor side opening plate 1-4, a colored glass filter 1-5, a two-dimensional sensor 1-6, Halogen lamps (light sources) that irradiate halogen lamps 1-7. Specifically, it is constituted as follows figure 1 optical system. in having The pinhole plate 1-1 of the pinhole is irradiated with a halogen light 1-7 from below, thereby forming of luminescence points 1-8. Additionally, will have The luminous point side opening plate 1-2 of the opening of the luminous point 1-8 is arranged at a position 0.2 mm away from the top of the luminous point 1-8, and a condenser lens 1-3 with a focal length of f=1.4 mm is disposed on the luminous point 1-8. 1.54mm above the position, will have The sensor side opening plate 1-4 of the opening is arra...
Embodiment 2
[0123] A model experiment was performed on the simplest case of an optical system that detects the fluorescence of a plurality of phosphors emitted from a plurality of light-emitting points in detection regions of a plurality of wavelength bands, respectively. In this embodiment and other embodiments using the same model experimental system, as an example, in (Math. 9) to (Math. 12), for A=2 light-emitting points, B=2 are respectively selected In the case of detecting the fluorescence of C=2 kinds of phosphors in the detection area of the wavelength band, it goes without saying that the same effect can be obtained by the same method when A, B, and C are respectively other values.
[0124] Figure 8 represents the model experimental system. Figure 8 The model experiment system of the present invention includes two capillaries Cap (1) and Cap (2) and a light source (not shown) that irradiates laser beam LB in the direction in which the two capillaries Cap (1) and Cap (2) are...
Embodiment 3
[0148] Next, according to the experimental results of [Example 2], the analysis of unknown samples was carried out this time. Figure 14 is to use Figure 8 The model experimental system, the original data obtained by injecting unknown samples into the capillary Cap(1) and Cap(2) for electrophoresis analysis. Since the same model experiment system as in [Example 2] was used, (Math. 18) to (Math. 22) can be used as it is. Expression method and Figure 11 same. but, Figure 11 The vertical axis scales of (b) and (d) are narrowed down to fluorescence intensity -0.5 to 2.0. At each of times 100, 200, 300, and 400, a large peak is observed at the luminous point P(1), while a small peak (indicated by an arrow) is observed at the luminous point P(2).
[0149] Figure 15 is directed at Figure 14 The original data of , and the color conversion data obtained by performing the color conversion of (Expression 18) and (Expression 19) at each time. Expression method and Figure 14...
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