Method for breeding virus-free fries of siniperca chuatsi
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A virus-free and seed-free technology, applied in antiviral agents, fish farming, pharmaceutical formulations, etc., can solve problems such as production management, unstable output, and impact on hatching rate, so as to improve hatching rate, prevent virus invasion, The effectiveness of enhanced biosecurity measures
Inactive Publication Date: 2021-12-28
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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Problems solved by technology
At present, the breeding of mandarin fish seedlings in my country mainly focuses on the spawning rate and hatching rate of the seedlings, and has not carried out production management from the level of disease prevention and control. As a result, the seedlings produced by many seedling enterprises have a high virus carrying rate, the hatching rate is affected, and the output unstable
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preparation example 1
[0036] A virus inactivator, comprising the following raw material components:
[0037] Polyhexamethylene biguanide: 30g;
[0038] Glutaraldehyde: 10g;
[0039] Rhubarb extract: 5g;
[0040] Chitosan: 3g;
[0041] Deionized water: 63g.
preparation example 2
[0043] A virus inactivator, comprising the following raw material components:
[0044] Polyhexamethylene biguanide: 20g;
[0045] Glutaraldehyde: 20g;
[0046] Rhubarb extract: 10g;
[0047] Chitosan: 2g;
[0048] Deionized water: 37g.
Embodiment 1
[0050] A kind of mandarin fish virus-free seed breeding method specifically comprises the following steps:
[0051] 1. Cultivation of backup parents without specific virus
[0052] 1.1. Selection of venue
[0053] In 2019, a batch of mandarin fish was bred in a mandarin fish farm in Qingyuan. The farm environment is remote and independent, and there are no other farmers around. There has been no mandarin fish virus disease in the past 3 years. The area of the mandarin fish backup parent breeding pond is 5 mu. A total of 10,000 mandarin fish reserve parent seedlings with a body length of 5cm-8cm were put in, and the stocking density was 1500-2000 / mu.
[0054] 1.2. Preparation before stocking of mandarin fish reserve parent seedlings
[0055]Virus detection was carried out before the seedlings of mandarin fish backup parents were put in, and it was confirmed that they did not carry mandarin fish frog iridescent virus, infectious spleen and kidney necrosis virus and mandarin ...
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Abstract
The invention discloses a method for breeding virus-free fries of siniperca chuatsi and relates to the technical field of aquaculture. The method comprises the following steps: selecting fries which do not carry viruses to breed backup parents, and carrying out immunization and regular virus detection; screening backup parents which do not carry viruses; selecting sexually mature backup parents, sequentially carrying out artificial spawning induction and artificial dry insemination, carrying out purification treatment on fertilized eggs by adopting a virus inactivating agent with the concentration of 20-50 ppm, rinsing the fertilized eggs by using clear water, and then hatching the fertilized eggs; and carrying out virus detection on the water spray fries which come out of the membrane and swim flatly, breeding siniperca chuatsi fries which do not carry viruses, carrying out virus detection during the breeding period, and obtaining virus-free fries of siniperca chuatsi if the result is negative. According to the method, the parents are screened by utilizing an advanced pathogen detection technology matched with a virus purification means, introduction and infection of viruses are eradicated, the siniperca chuatsi fries are prevented from carrying the viruses, the quality of the siniperca chuatsi fries is improved, and stable and healthy development of the siniperca chuatsi industry is facilitated.
Description
technical field [0001] The invention relates to the field of aquaculture, in particular to a method for breeding mandarin fish virus-free seedlings. Background technique [0002] Mandarin fish (Siniperca chuatsi), also known as sweet-scented osmanthus fish, belongs to Perciformes (Perciformes), Serranidae (Serranidae), and Siniperca (Siniperca). like. China's annual output of mandarin fish in 2020 will be about 350,000 tons, with an annual output value of over 20 billion yuan. However, with the expansion of the scale of mandarin fish farming, the increase of the degree of intensive farming, the increase of the breeding density and the deterioration of the aquaculture water environment, the diseases of mandarin fish are becoming more and more serious, especially the problem of viral diseases of mandarin fish is becoming more and more prominent. Viral diseases caused by iridescent virus, infectious spleen-kidney necrosis virus and mandarin fish bullet virus have become one o...
Claims
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