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Method for preparing keratin solution by hydrolyzing wool

A technology for hydrolyzing keratin and keratin, which is applied in the field of wool hydrolysis to prepare keratin solution, which can solve the problems of low degradation rate, excessive molecular weight of the product, poor odor and stability, etc., and improve hair damage and film-forming properties good effect

Pending Publication Date: 2021-12-31
上海威之信生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that these methods have certain shortcomings, such as excessive product molecular weight, low degradation rate, product purity, color, odor and poor stability, etc., and the obtained products cannot be used in cosmetics.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Soak 20g of wool in 100g of sulfuric acid solution with a mass fraction of 3%, keep it warm at 90°C for 4 hours, then cool down to room temperature and filter to remove the acid solution, then rinse with 500g of deionized water and dry to obtain acidification wool;

[0020] (2) Place the wool obtained in step (1) in 100 g of deionized water, and slowly add Na at a concentration of 0.5% under ultrasonic conditions 2 CO 3 , adjust the pH of the solution to stabilize at about 10.0, and filter;

[0021] (3) Raise the temperature to 45°C, adjust the pH to 10.0, add acidified wool with a mass percentage of 15% of Bacillus subtilisin for enzymatic hydrolysis, keep the pH at about 10.0 for enzymatic hydrolysis for 16 hours, and inactivate the enzyme after enzymatic hydrolysis, raise the temperature to 90°C and maintain for 0.5h ;

[0022] (4) Add 1g of activated carbon in step (3), stir and decolorize for 0.5h at a temperature of 90°C, and filter after decolorization to ...

Embodiment 2

[0025] (1) Soak 10g of wool in 100g of hydrochloric acid solution with a mass fraction of 10%, keep it warm at 70°C for 12 hours, then cool down to room temperature and filter out the acid solution, then rinse with 500g of deionized water and dry to obtain acidified wool;

[0026] (2) Put the wool obtained in step (1) in 100g of deionized water, slowly add NaOH with a concentration of 0.2% dropwise under ultrasonic conditions, adjust the pH of the solution to be stable at about 9.5, and filter;

[0027] (3) Raise the temperature to 55°C, adjust the pH to 9.5, add acidified wool with a mass percentage of 10% Bacillus subtilisin for enzymatic hydrolysis, keep the pH at about 9.5 for enzymatic hydrolysis for 48 hours, and inactivate the enzyme at the end of the enzymatic hydrolysis, and raise the temperature to 80°C for 1 hour;

[0028] (4) Add 0.7g of activated carbon in step (3), keep stirring at 80°C for 2 hours for decolorization, and filter after decolorization to obtain a li...

Embodiment 3

[0031] (1) Soak 5g of wool in 100g of acetic acid solution with a mass fraction of 15%, keep it warm at 60°C for 24 hours, then cool down to room temperature and filter to remove the acid solution, rinse with 500g of deionized water, and dry to obtain acidified wool;

[0032] (2) Place the wool obtained in step (1) in 100g of deionized water, slowly add KOH with a concentration of 0.5% dropwise under ultrasonic conditions, adjust the pH of the solution to be stable at about 8, and filter;

[0033] (3) Raise the temperature to 75°C, adjust the pH to 8, add acidified wool with a mass percentage of 1.5% subtilisin for enzymatic hydrolysis, keep the pH at about 8 for enzymatic hydrolysis for 72 hours, and inactivate the enzyme at the end of the enzymatic hydrolysis, and raise the temperature to 70°C for 2 hours;

[0034] (4) Add 0.1 g of activated carbon to step (3), stir and decolorize for 4 hours at 60°C, and filter after decolorization to obtain a light yellow clear hydrolyzed k...

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Abstract

The invention discloses a method for preparing a keratin solution by hydrolyzing wool. The method comprises the following steps: soaking wool in an acid solution with a certain mass percentage concentration, heating and softening, cooling, washing with water, and drying to obtain acidified wool; adding the obtained acidified wool into water, dropwise adding alkali liquor under an ultrasonic condition to adjust the pH value to 8.0-10.5, and filtering; adopting subtilisin to conduct enzymolysis on the pretreated wool, conducting enzyme deactivation after enzymolysis is finished, adding activated carbon for decoloration, and filtering to obtain a hydrolyzed wool keratin dilute solution; and concentrating the hydrolyzed wool keratin dilute solution on concentration equipment to obtain the hydrolyzed keratin solution. According to the method, the hardness of wool keratin is reduced through acidification pretreatment, the difficulty of wool enzymolysis is reduced, and the molecular weight of hydrolyzed keratin is also reduced while the wool enzymolysis efficiency is improved. The method is simple in process and convenient to operate, the wool dissolution rate can reach 70% or above, and the extraction rate can reach 60% or above.

Description

technical field [0001] The invention belongs to the technical field of keratin preparation methods, and in particular relates to a method for preparing keratin solution by hydrolyzing wool. Background technique [0002] Wool contains more than 80% α-keratin, and its structure is very similar to keratin in human hair. Wool is hydrolyzed to produce low molecular weight hydrolyzed keratin, which can be used as a hair conditioner and applied to hair care products middle. Since the molecular structure of keratin contains a large number of disulfide bonds, cross-linking occurs within and between peptide chains, forming a stable three-dimensional network structure, so there are certain difficulties in the degradation of keratin macromolecules. At present, the wool dissolution methods mainly include mechanical method, oxidation method, reduction method, acid-base method, metal salt method and cuproammonia solution method. Studies have shown that these methods have certain shortcom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/65A61Q19/10A61Q5/02C12P21/06
CPCA61K8/65A61Q19/10A61Q5/02C12P21/06A61K2800/805
Inventor 杨瑞强王晓莉诸冬寒刘新贵
Owner 上海威之信生物技术有限公司
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