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A kind of combined fermented product, skin external preparation containing same, preparation method and application thereof

A combination of fermentation and fermentation substrate technology, applied in the field of fermentation, can solve the problems of low extraction rate of active ingredients, complex preparation process, high production cost, etc., and achieve the effect of large-scale industrial production, stable fermentation process and high safety

Active Publication Date: 2022-07-01
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the complex preparation process and high preparation cost of cactus extract and aloe extract in the prior art; the extraction rate of active components is low, resulting in waste of resources; the addition of chemical reagents in the extraction process pollutes the environment; Severely limit the application of the two in the field of cosmetics and other defects, and provide a combined fermented product, an external skin preparation containing it, and its preparation method and application

Method used

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  • A kind of combined fermented product, skin external preparation containing same, preparation method and application thereof
  • A kind of combined fermented product, skin external preparation containing same, preparation method and application thereof
  • A kind of combined fermented product, skin external preparation containing same, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0063] 1. Weigh 1g and 10g of peeled cactus aloe vera solution and 100g of deionized water in a homogenizer, place the homogenate in a 500mL conical flask, add 100g of deionized water to the homogenizer, rinse the residue The homogenate was transferred to a 500mL conical flask, sealed with parafilm, and then sterilized under high temperature and high pressure at 121°C and 0.12Mpa for 30min; after sterilization, let stand at room temperature to obtain fermentation substrate;

[0064] 2. Dissolve Lactobacillus delbrueckii subsp. bulgaricus in sterile deionized water to obtain a concentration of 10 7 -10 8 CFU / g Lactobacillus delbrueckii subsp. bulgaricus bacterial liquid, take 15 g of dissolved bacterial liquid and inoculate it in the fermentation substrate obtained in the above step 1, and seal it with parafilm; the temperature is 40 ° C, and the rotating speed is 180 r / min Cultivated in the constant temperature shaking incubator for 15h to obtain the crude product of the com...

Embodiment 2

[0067] 1. Weigh 1g of peeled cactus, 1g of aloe vera liquid and 100g of deionized water and homogenize them in a homogenizer, place the homogenate in a 500mL conical flask, add 100g of deionized water to the homogenizer, rinse the residue The homogenate was transferred to a 500mL conical flask, sealed with parafilm, and then sterilized under high temperature and high pressure at 121°C and 0.12Mpa for 30min; after sterilization, let stand at room temperature to obtain fermentation substrate;

[0068] 2. Dissolve Lactobacillus delbrueckii subsp. bulgaricus in sterile deionized water to obtain a concentration of 10 7 -10 8 CFU / g Lactobacillus delbrueckii subsp. bulgaricus bacterial liquid, take 15 g of dissolved bacterial liquid and inoculate it in the fermentation substrate obtained in the above step 1, and seal it with parafilm; the temperature is 40 ° C, and the rotating speed is 180 r / min Cultivated in the constant temperature shaking incubator for 15h to obtain the crude p...

Embodiment 3

[0071] 1. Weigh 1g and 40g of aloe vera liquid of peeled cactus and 100g of deionized water in a homogenizer, place the homogenate in a 500mL conical flask, add 100g of deionized water to the homogenizer, rinse the residual The homogenate was transferred to a 500mL conical flask, sealed with parafilm, and then sterilized under high temperature and high pressure at 121°C and 0.12Mpa for 30min; after sterilization, let stand at room temperature to obtain fermentation substrate;

[0072] 2. Dissolve Lactobacillus delbrueckii subsp. bulgaricus in sterile deionized water to obtain a concentration of 10 7 -10 8 CFU / g Lactobacillus delbrueckii subsp. bulgaricus bacterial liquid, take 15 g of dissolved bacterial liquid and inoculate it in the fermentation substrate obtained in the above step 1, and seal it with parafilm; the temperature is 40 ° C, and the rotating speed is 180 r / min Cultivated in the constant temperature shaking incubator for 15h to obtain the crude product of the c...

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Abstract

The invention discloses a combined fermented product, a skin external preparation containing the same, and a preparation method and application thereof. The preparation method of the combined fermented product comprises the following steps: Lactobacillus delbrueckii subsp. bulgaricus is inoculated into a fermentation substrate, fermented and cultured, and then sterilized; wherein, the fermentation substrate includes cactus and aloe vera liquid; Lactobacillus delbrueckii bulgaricus liquid; The subspecies (Lactobacillus delbrueckii subsp.bulgaricus) was purchased from the China General Microbial Culture Collection and Management Center, and the deposit number is CGMCC No. 1.1480. The combined fermentation product of the invention has wide raw material sources, simple preparation process, low preparation cost and wide application; no external chemical reagents need to be added in the process, thereby reducing environmental pollution; during the extraction process, the loss of active components is reduced, and the fermentation product has ideal resistance to Oxidative effect, moisturizing effect and anti-allergic effect, high safety to use.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a combined fermented product, an external preparation for skin containing the same, and a preparation method and application thereof. Background technique [0002] With the changes of age, environment and season, the water content of the stratum corneum and the state of the skin will change. When the water content of the stratum corneum is less than 10%, the skin will be dry, and the dry state of the skin will accelerate skin aging. When the human body is affected by exogenous factors (ultraviolet rays, environmental toxins, etc.), excess reactive oxygen species will be produced in the body, which will lead to the occurrence of oxidative stress and accelerate skin aging. With people's pursuit of nature and health, natural plant raw materials are favored by people. While enhancing the appearance of the human body, natural plant raw materials are more biocompatibl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9794A61K8/9789A61Q19/00A61Q19/08
CPCA61K8/9794A61K8/9789A61Q19/00A61Q19/08A61Q19/005A61K2800/85A61K2800/782A61K2800/5922
Inventor 王冬冬李萌王昌涛张佳婵赵丹
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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