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Biomarker for diabetes diagnosis and application thereof

A technology for biomarkers, diabetes, applied in the field of biochemistry

Pending Publication Date: 2021-12-31
上海脉示生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no metabolic markers relevant to the diagnosis of diabetes

Method used

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  • Biomarker for diabetes diagnosis and application thereof
  • Biomarker for diabetes diagnosis and application thereof
  • Biomarker for diabetes diagnosis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0204] 1. The source of the specimen

[0205] After obtaining the consent of the patients, the plasma samples of 29 clinically diagnosed diabetic patients (fasting blood glucose>7.0mmol / L, two-hour postprandial blood glucose>11.1mmol / L) and 29 healthy people were collected, and the age , gender and diabetes matched.

[0206] 2. Main instruments and equipment:

[0207] Shimadzu LC-MS 8050; LabSolutions Insight software.

[0208] 3. Reagents and materials:

[0209] Acetyl-L-carnitine, Betaine, Choline Chloride, Crotonobetaine Hydrochloride, γ-Butyl Betaine, L-Carnitine, Trimethylamine Oxide Standards; Acetyl-L-Carnitine-D3, γ-Butyl Betaine-D9 , Betaine-D11, Choline Chloride-D9, L-Carnitine-D9, Trimethylamine Oxide-D9, Methanol, Acetonitrile, Ethanol, Formic Acid, Ammonia, and Carbon Adsorption Human Plasma are all commercially available reagents.

[0210] 4. Liquid chromatography and mass spectrometry analysis conditions:

[0211] Chromatographic conditions:

[0212] Colum...

Embodiment 2

[0240] Example 2: Constructing a ROC curve to compare the ability of the above intestinal flora metabolites alone or in combination to diagnose diabetes patients and healthy people

[0241] The receiver operating curve (ROC) method was used for verification, and the acetyl-L-carnitine, betaine, choline chloride, crotonobetaine hydrochloride, and γ-butyl beet in 29 cases of diabetic samples and 29 cases of non-diabetic normal plasma samples were verified. The expression levels of alkali, L-carnitine and trimethylamine oxide can be used to judge their ability to diagnose diabetes. According to the sensitivity and specificity data automatically derived by the ROC curve method, it is assumed that the specificity of all indicators is 96.6% (that is, only one case is positive in healthy samples), and the sensitivity, AUC and threshold obtained from the ROC curve are shown in Table 2. .

[0242] Table 2 The ability of a single differential metabolite diagnosis to distinguish diabeti...

Embodiment 3

[0247] In order to further verify whether acetyl-L-carnitine, which is first used in the diagnosis of diabetic patients and healthy people, has a joint detection effect with the two indicators of choline chloride and trimethylamine oxide.

[0248] Table 3 The ability of the combined diagnosis of two differential metabolites to distinguish diabetic patients from healthy people

[0249]

[0250] As shown in Table 3, when acetyl-L-carnitine and choline chloride are used in combination, the ability to diagnose and distinguish diabetic patients from healthy people can be significantly improved. In the case of single detection, the sensitivity increased from 82.8% and 75.9% in single detection to 96.6% in joint detection. When acetyl-L-carnitine is combined with TMAO, its diagnostic ability to distinguish diabetic patients from healthy people is not as good as the combination of acetyl-L-carnitine and choline chloride, with an AUC of 0.96; when the specificity is 96.6%, the sensi...

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Abstract

The invention provides a biomarker for diabetes diagnosis and application thereof. Specifically, the invention provides a biomarker set, and the biomarker set comprises one or more of acetyl L-carnitine, trimethylamine oxide and choline chloride. The acetyl-L-carnitine is applied to diagnosis of diabetes for the first time. Through verification, when the acetyl-L-carnitine is used for diagnosing and distinguishing a diabetic patient and a healthy person, the AUC reaches 0.8 or above, and when the two indexes of the acetyl-L-carnitine and choline chloride are jointly used, the AUC is closer to 1 than a single index, and the diagnosis effect is better; when the three indexes of acetyl-L-carnitine, trimethylamine oxide and choline chloride are jointly applied, the AUC is closer to 1 than that of a single index and two indexes which are jointly applied, and the diagnosis distinguishing effect is the best. Diabetes can be accurately diagnosed with high specificity and high sensitivity by detecting the biomarker provided by the invention.

Description

technical field [0001] The invention belongs to the field of biochemistry, relates to disease diagnosis markers, in particular to biomarkers (groups) used for diabetes diagnosis. Background technique [0002] With the changes in people's lifestyles, the improvement of living standards and the increase of the elderly population, the incidence of diabetes worldwide continues to grow. According to the latest global diabetes map released by the International Diabetes Federation: In 2019, the countries with the largest number of adults with diabetes, China continues to rank first and is expected to continue until 2030. High incidence, low diagnosis rate and low control rate are the current status of diabetes in my country. Diabetes mellitus is a group of metabolic syndromes with chronic hyperglycemia as the common clinical feature. The cause of hyperglycemia is the impairment of glucose utilization by the body due to many factors such as insulin secretion defects and / or insulin re...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/34G01N30/86
CPCG01N30/02G01N30/34G01N30/8679
Inventor 詹红铃木亨霍志远朱凤
Owner 上海脉示生物技术有限公司
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