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Marker for predicting lymph node metastasis of medullary thyroid carcinoma and application of marker

A technology of lymph node metastasis and thyroid, applied in the field of genetic engineering

Inactive Publication Date: 2022-01-04
AFFILIATED HOSPITAL OF YOUJIANG MEDICAL UNIV FOR NATTIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no effective detection method or means in the clinical field to provide corresponding support for clinicians

Method used

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  • Marker for predicting lymph node metastasis of medullary thyroid carcinoma and application of marker
  • Marker for predicting lymph node metastasis of medullary thyroid carcinoma and application of marker
  • Marker for predicting lymph node metastasis of medullary thyroid carcinoma and application of marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042]1. Patients with medullary thyroid carcinoma were divided into two groups, one group was patients without lymph node metastasis, and the other group was patients with lymph node metastasis. Single-cell transcriptome sequencing was performed on the resected cancer tissues. The expression level of SNX20 gene in cell clusters in the above two groups of cancer tissues was shown by the gene characteristic map, and the results are as follows figure 1 shown.

[0043] 2. Patients with medullary thyroid carcinoma were divided into two groups, one group was patients without lymph node metastasis, and the other group was patients with lymph node metastasis. Single-cell transcriptome sequencing was performed on the resected cancer tissues. The gene violin plot was used to display the expression level of SNX20 gene in the above two groups of cancer tissues, and the results were as follows figure 2 shown.

[0044] according to figure 1 and figure 2 The detection results showed t...

Embodiment 2

[0046] 1. Design of primers for RT-qPCR detection

[0047] The SNX20 primers used for RT-qPCR in this application were designed according to conventional methods. That is, download the human SNX20 gene sequence in the ENSEMBL database, import the downloaded sequence into the NCBI PrimerBlast online primer design software, set the RANGE and the size of the PCR product, and then click GET PRIMERS below, after the database background operation and calculation, the feedback obtained the primer sequence.

[0048] In the present invention, the primer sequence used to detect the expression level of SNX20 gene by RT-qPCR technology is: upstream primer: 5'-ACCTGACGGGCACTTAGACA-3' (SEQ ID NO.2); downstream primer: 5'-AGAGCAGTTTGACGTGCTTCC-3' (SEQ ID NO.3). The size of the amplified fragment was 119bps. The above sequences can be synthesized by biological companies and commercially available.

[0049] 2. Immunofluorescence imaging technology detects the antibody required for the expr...

Embodiment 3

[0052] Example 3 Association between SNX20 gene expression and lymph node metastasis in patients with medullary thyroid carcinoma

[0053] 1. Sample processing

[0054] The surgically stripped medullary thyroid cancer samples with lymph node metastasis were divided into two parts. The first part was used for RNA extraction; the second part was embedded for cryosectioning.

[0055] (1) RNA isolation Total RNA was isolated using TRIzol reagent according to the product instructions. RNA purity, concentration and integrity were assessed using Nanodrop 2000 Spectrophotometer (Thermo Scientific), 2100 Bioanalyzer (RNA6000 NanoLabChip; Agilent Technologies, Santa Clara, CA), agarose gel electrophoresis.

[0056] (2) Frozen sections: Embed with embedding agent (LEICA FSC22 Clear) according to the product instructions, place on top of liquid ammonia and fix slowly. The fixed samples were sliced ​​using a cryostat (LEICA CM1950) within 12 hours, and the thickness of the slices should...

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Abstract

The invention discloses a marker for predicting lymph node metastasis of medullary thyroid carcinoma and application of the marker. The marker is SNX20. By detecting the expression level of the SNX20, auxiliary diagnosis and prognosis of the medullary thyroid carcinoma and / or prediction of the lymph node metastasis of the medullary thyroid carcinoma can be carried out. According to the scheme recorded by the invention, the risk of the lymph node metastasis of the medullary thyroid carcinoma of an individual with high SNX20 expression is lower than that of an individual with low SNX20 expression.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a marker for predicting lymph node metastasis of medullary thyroid carcinoma and its application. Background technique [0002] Medullary thyroid cancer is a cancer that forms in the thyroid gland. The thyroid is a gland at the front of the neck that is responsible for sending hormones to the rest of the body. Clinically, medullary thyroid carcinoma is very easy to spread through the lymphatic system and metastasize to other organs, which seriously affects the clinical treatment effect of the disease. Therefore, timely detection and prediction of whether medullary thyroid carcinoma will metastasize to the lymphatic system can provide important reference information for clinicians, formulate and adjust treatment strategies for patients in time, and improve the clinical diagnosis and treatment of medullary thyroid carcinoma. However, there is no effective ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/12C12N15/11
CPCC12Q1/6886C07K14/47C12Q2600/158
Inventor 卢冠铭孟令章陆金兰韦忠恒马燕飞陈永诚罗志斋黄前方余艳蓉杨录新梁慧韦莲萍玉奇生李新梅陆美新
Owner AFFILIATED HOSPITAL OF YOUJIANG MEDICAL UNIV FOR NATTIES
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