Unlock instant, AI-driven research and patent intelligence for your innovation.

Atlantibacter hermannii for degrading methyl mercury and application thereof

A technology of methylmercury and bacilli, applied in the field of microorganisms, can solve problems such as limited understanding of biodegradation

Active Publication Date: 2022-01-18
HUAZHONG AGRI UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some research progress has been made on the abiotic degradation of methylmercury, but the understanding of biodegradation is relatively limited.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Atlantibacter hermannii for degrading methyl mercury and application thereof
  • Atlantibacter hermannii for degrading methyl mercury and application thereof
  • Atlantibacter hermannii for degrading methyl mercury and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1. Screening and identification of strains

[0015] The applicant, Huazhong Agricultural University, obtained a strain of unknown classification and function from the China Agricultural Microorganism Culture Collection Management Center, recovered and expanded the culture, and sent it to Shanghai Meiji Biomedical Technology Co., Ltd. for whole genome extraction and sequencing of amplified products . The obtained gene sequences were homologously compared by GeneBank to determine their taxonomic status.

[0016] The result shows that its gene sequence is 99% similar to the 16S rRNA gene sequence of Atlantibacter hermannii. The bacterial strain belongs to Atlantibacter hermannii. The sequencing result is shown in SEQ ID No.1.

[0017] The applicant classified and named the strain Atlantibacter hermannii L-1, and sent it to the Chinese Type Culture Collection Center (CCTCC) of Wuhan University, Wuhan City, Hubei Province on October 26, 2021 for preservation. The n...

Embodiment 2

[0018] Functional verification of the strain of Example 2

[0019] 1. Recovery and expansion of strains

[0020] (1) Open the ampoule tube of freeze-dried bacteria in an aseptic operation, add an appropriate amount (0.3ml) of YM agar liquid medium to the freeze-dried powder of the bacteria with a sterile pipette, and shake gently to dissolve the freeze-dried bacteria into a Suspended. Aspirate all the bacterial suspensions, transplant them into slant culture medium (no more than 2 tubes), and culture them statically at 28°C. This is the first-generation culture;

[0021] (2) Take the first-generation culture with an inoculation loop, inoculate it in YM liquid medium, and shake it in a shaker at 28°C for 24 hours, which is the second-generation culture, and cultivate it to the required number of generations in this way.

[0022] 2. Functional verification of strains

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an Atlantibacter hermannii L-1 for degrading methyl mercury, which is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number of the Atlantibacter hermannii L-1 is CCTCC NO: M 20211329. The Atlantibacter hermannii has strong degradation capacity on methyl mercury, the degradation rate of the methyl mercury with the final concentration of 1 ng ml<-1> can reach 31.99%, and the degradation rate can be further improved under the condition that Ca <2+> is added. The Atlantibacter hermannii provided by the invention can be applied to degradation of methyl mercury in environmental soil, and has important significance on reduction of methyl mercury residues in crops and protection of life health of people and animals.

Description

technical field [0001] The invention relates to a strain of Atlantibacter hermannii for degrading methylmercury and an application thereof, belonging to the field of microorganisms. Background technique [0002] Methylmercury is the most toxic organic form of heavy metal mercury. It has strong bioaccumulation and biomagnification characteristics, and it is easy to threaten food safety and life and health along the food chain. The total economic loss caused by methylmercury ingestion by humans worldwide today is 117 billion US dollars, so the regulation of methylmercury content is very important. The degradation of methylmercury in the natural environment is mainly through biological and abiotic degradation. At present, some research progress has been made on the abiotic degradation of methylmercury, but the understanding of biodegradation is relatively limited. The environment is rich in microbial resources, and many microorganisms can not only degrade pollutants in the en...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10A23L5/20C12R1/01
CPCB09C1/10A23L5/28
Inventor 刘玉荣周心劝刘琴郝芸芸
Owner HUAZHONG AGRI UNIV