A fluorescent probe for quickly identifying mercury and methylmercury and its preparation method and application

A fluorescent probe, methylmercury technology, applied in the field of analytical chemistry, can solve problems such as the selective signal of two ions that are difficult to detect, and achieve the effect of improving detection sensitivity

Active Publication Date: 2021-04-20
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the currently reported probes show the emission of Hg(II) or MeHg always at the same wavelength, making it difficult to simultaneously detect the selective signals of the two ions in vivo.

Method used

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  • A fluorescent probe for quickly identifying mercury and methylmercury and its preparation method and application
  • A fluorescent probe for quickly identifying mercury and methylmercury and its preparation method and application
  • A fluorescent probe for quickly identifying mercury and methylmercury and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0052] Example 1: Preparation of small molecule probes

[0053] (1) Dissolve 2-bromo-4'-hydroxyacetophenone (5mmol, 1.075g), 4-dimethylaminobenzaldehyde (5mmol, 0.745g), sodium carbonate (10mmol, 1.060g) in 20ml of ethanol . Then add 5ml of water and stir at 60°C for 6h. After the reactant is cooled, add hydrochloric acid (3ml, 12m) and stir until neutral. The solid was filtered off, and the insoluble matter in the solution was removed after washing with ethanol. The solution was then rotovaped to remove solvent. Product is orange-red solid (1.005g) (55.2%), and wherein, the H spectrum (A), C spectrum (B) of compound BHDP are as figure 2 shown;

[0054] (2) BHDP (1mmol, 0.534g), 4-formylphenylboronic acid (1mmol, 0.149g), potassium carbonate (14mmol, 1.932g), palladium(II) acetate (0.05mmol, 0.011g), triphenylphosphine (0.05 mmol, 0.013 g) was dissolved in a mixture of 1,4-dioxy (20 mL) and water (5 mL). Then, under nitrogen protection, reflux at 80°C for 6h. The solve...

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Abstract

The invention belongs to the field of analytical chemistry, and relates to a fluorescent probe for quickly identifying mercury and methylmercury, a preparation method and an application thereof. The structural formula of the fluorescent probe is as follows:. The fluorescent probe prepared by the present invention improves the detection sensitivity without adding any other additional materials, and avoids the addition of additional materials, reduces the consumption of additional materials and reduces the source of errors in the detection; in the detection The imaging of the analyte in living cells and zebrafish has been successfully detected, and it is suitable for real-time monitoring and differentiation of cells and zebrafish in vivo. This method has not been able to do it in the previous method. The realization of imaging has greatly promoted the in-depth study of distinguishing mercury from methylmercury.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and relates to a fluorescent probe for quickly identifying mercury and methylmercury, a preparation method and an application thereof. Background technique [0002] At present, most of the researches on Hg(II) and MeHg are indirect reports in hypoxic environment in vitro. Most of the methods for monitoring Hg(II) and MeHg are indirect separation or derivatization of the two ions in vitro. The pretreatment and conventional chromatographic separation are very time-consuming, which makes the in vivo and real-time detection of the two ions impossible. Fluorescence imaging provides a powerful methodological support for studying the physiology and pathology of ions in their natural environment with minimal disturbance to biological systems. Therefore, fluorescence imaging using biocompatible dual-responsive fluorescent probes is an ideal approach to detect and differentiate Hg(II) or MeHg. However...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C225/22C07C221/00C09K11/06G01N21/64
CPCC07C221/00C07C225/22C09K11/06C09K2211/1007G01N21/6428G01N21/6486
Inventor 那娜付强欧阳津
Owner BEIJING NORMAL UNIVERSITY
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