Fluorescent ion imprinted sensor and application thereof in methyl mercury ion detection

A fluorescent ion and sensor technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., to achieve the effects of no contamination of samples, easy storage, and good stability

Active Publication Date: 2013-11-06
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Ion imprinting technology uses metal ions as templates to prepare ion imprinted polymers, which have the advantages of specific recognition sites, high anti-interference and resistance to harsh external conditions, and are mainly used in solid phase extraction and the separation of metal ions in actual samples analysis, but few reports have been applied to the field of fluorescent sensors

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  • Fluorescent ion imprinted sensor and application thereof in methyl mercury ion detection

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Experimental program
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Effect test

Embodiment 1

[0043]Dissolve 0.004mmol 8-mercaptoquinoline and 0.002mmol methylmercury chloride in 1.5mlDMSO, mix and sonicate for 20min; Azodiisobutyronitrile was mixed thoroughly and ultrasonically mixed for 30 minutes to obtain a reaction solution; a cellulose acetate film of 1 cm length × 1 cm width × 0.2 μm thickness was placed in the above reaction solution for 30 minutes, then taken out, clamped with a quartz plate, and After the air bubbles were released, the quartz plate was wrapped and sealed with a parafilm, and then heated in a water bath at 65°C for 15 hours. After the reaction is complete, take out the prepared methylmercury ion imprinted membrane, first elute with 0.1M EDTA aqueous solution, and then use 0.1M sulfuric acid solution to elute the template ions until the fluorescence intensity no longer changes, and then use methanol and ultrapure Eluted with water, and finally dried the imprinted membrane, which is the fluorescent ion imprinted sensor. The optimal pH of the ob...

Embodiment 2

[0045] Dissolve 0.002mmol of methylmercury chloride and 0.004mmol of 2-naphthyl acrylate in 1ml of DMSO, mix and sonicate for 30min, then add 0.4mmol of 4-vinylpyridine, 2mmol of trimethoxypropane trimethacrylate and 0.01g of azo Diisobutyronitrile, ultrasonically mixed for 20 minutes to obtain a reaction solution; put a polypropylene film of 1 cm length × 1 cm width × 0.47 μm thickness in the above reaction solution for 20 minutes, take it out, clamp it with a quartz plate, drive out the air bubbles, and use After sealing the parafilm, heat in a water bath at 65°C for 10 hours to prepare a methylmercury ion fluorescent imprinted membrane, take the membrane out of the quartz plate, and wash it with 0.1M HNO 3 The solution elutes the template ions on the imprinted membrane until the fluorescence intensity no longer changes, and then washes away the residual HNO with ultrapure water 3 solution, and the imprinted membrane is dried to obtain a fluorescent ion imprinted sensor. Th...

Embodiment 3

[0047] Mix 0.006mmol of 1-allyl-3-(1-naphthyl)-2-thiourea and 0.002mmol of methylmercury bromide in 1.5ml of DMSO and sonicate for 30min, then add 0.4mmol of 2-vinylpyridine, 3.2 One mmol ethylene glycol dimethacrylate cross-linking agent and 0.02 g of azobisisobutyronitrile were ultrasonically mixed for 30 minutes to obtain a reaction solution; a cellulose acetate film of 1 cm length × 1 cm width × 0.65 μm thickness was placed in the above reaction solution Take it out after standing in the medium for 10 minutes, clamp it with a quartz plate, drive out the air bubbles, seal it with a sealing film, and heat it in a water bath at 65°C for 6 hours to prepare a methylmercury ion fluorescent imprinted membrane. Take the membrane out of the quartz plate, and use 0.1M HNO 3 The solution elutes the template ions on the imprinted membrane until the fluorescence intensity remains unchanged, and washes away the residual HNO with ultrapure water 3 Afterwards, the imprinted membrane is d...

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Abstract

The invention provides an ion imprinted fluorescent sensor for selectively detecting methyl mercury ions and a preparation method for the ion imprinted fluorescent sensor. The sensor is prepared by the following three processes of: (1) forming a pre-copolymer by using the methyl mercury ions, a functional monomer and a fluorescent material through coordination; (2) adding a cross linker and an initiator, and performing initiated polymerization under proper conditions by using a microporous membrane as a supporting medium to immobilize template molecules in the polymer in a complementary spatial structure form; and (3) removing the template molecules from the polymer in an acid or ethylene diamine tetraacetic acid (EDTA) eluting mode, leaving specific holes, and thus obtaining the fluorescent methyl mercury ion imprinted composite membrane sensor. The obtained imprinted composite membrane shows high selectivity and sensitivity to methyl mercury ions, and can be reused for multiple times; and the fluorescent sensor is easy and fast to prepare, and can be used for selective detection of the methyl mercury ions in samples of environment and the like.

Description

(1) Technical field [0001] The invention relates to a fluorescent ion imprint sensor for detecting methylmercury ions and a preparation method thereof. (2) Background technology [0002] Mercury is a harmful element that widely exists in the environment. It exists in different forms and has different toxicity. Generally speaking, organic mercury is more toxic than inorganic mercury, and methylmercury is the most toxic form of organic mercury. One, after invading the human body, it will cause serious damage to the nervous system, heart, kidneys, skin, etc. The determination methods of methylmercury mainly include methods such as atomic absorption spectrometry, atomic emission spectrometry, gas chromatography, high performance liquid chromatography, ion chromatography and capillary electrophoresis, but these methods require relatively expensive testing instruments, and the testing cost higher. Fluorescence analysis has high sensitivity, easy operation and low cost, and has b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/42C08J9/26C08J7/18C08J7/16C08L27/16C08L1/12C08L23/12C08L81/06G01N21/64
Inventor 高云玲沈绍传姚克俭
Owner ZHEJIANG UNIV OF TECH
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