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A kind of c3 symmetry fluorescent molecular probe and its preparation method and application

A fluorescent molecular probe and fluorescent molecular technology, applied in the field of metal ion analysis and detection, can solve problems such as damage and human poisoning, and achieve the effects of good selectivity, low cost and good application prospects.

Inactive Publication Date: 2014-10-08
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, once the concentration of copper exceeds 1 mg / L, it will cause toxicity and damage to the human body

Method used

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  • A kind of c3 symmetry fluorescent molecular probe and its preparation method and application
  • A kind of c3 symmetry fluorescent molecular probe and its preparation method and application
  • A kind of c3 symmetry fluorescent molecular probe and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: Synthesis of fluorescent molecular probe L

[0025] Dissolve 140.6 mg of triaminoguanidine chloride (1 mmol) in a mixed solution of 4 mL of ethanol and 2 mL of water, and heat to reflux until completely dissolved. Then, 471.6 mg quinoline-2-carbaldehyde (3 mmol) was added dropwise to the above solution, and the reflux was continued for 4 h. After the reaction, the resulting scarlet solution was cooled to room temperature and stirred overnight. The solvent was removed, the residue was dissolved with an appropriate amount of absolute ethanol, and then anhydrous ether was added dropwise until all the scarlet precipitates were precipitated. Suction filtration, the precipitate was washed several times with anhydrous ether, and vacuum-dried to obtain a fluorescent molecular probe.

[0026] The characterization data is:

[0027] .

Embodiment 2

[0028] Embodiment 2: Specific application of fluorescent molecular probe L of the present invention to copper ions

[0029] The synthesized fluorescent molecular probe L was prepared as a mother solution with a 5% DMSO aqueous solution, and then diluted to a corresponding concentration with a buffer solution (50 mM HEPES, pH=7.40) for analysis and testing.

[0030] [Fluorescence titration: at the above 1 x 10 -5 М Fluorescent Molecular Probe L buffer solution, titrated with 0 to 3 equivalents of copper ion aqueous solution, excited with 360 nm light, and detected the change of fluorescence spectrum at 504 nm. From figure 1 It can be seen that, at 0 equivalent, probe L has a maximum emission at 504 nm. With the addition of copper ions, the fluorescence of fluorescent molecular probe L is gradually quenched; when 3 equivalents of copper ions are added, the fluorescence of fluorescent molecular probe L is completely quenched, which shows that the coordination of fluorescent mo...

Embodiment 3

[0033] Embodiment 3: Specific application of fluorescent molecular probe L of the present invention to cadmium ions

[0034] The synthesized fluorescent molecular probe L was prepared into a mother liquor with redistilled chloroform, and then diluted with chloroform to a corresponding concentration for analysis and testing.

[0035] Fluorescence titration: dilute high-concentration fluorescent molecular probe L with redistilled chloroform to a solution of 5 μМ, titrate with methanol solution of 0 to 3 equivalents of cadmium ions, excite with 370 nm light, and detect at 543 nm and 599 nm changes in the fluorescence spectrum. From Figure 4 It can be seen from the figure that at 0 equivalent, the fluorescent molecular probe L has a maximum emission at 543 nm. With the addition of cadmium ions, the fluorescence gradually increased and red-shifted to 599 nm; when 3 equivalents of cadmium ions were added, the fluorescence intensity reached the maximum value, and the fluorescence ...

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Abstract

The invention discloses a C3 symmetric fluorescent molecular probe as well as its preparation method and application, and belongs to the technical field of metal ion analysis and detection. The structural formula is: it has good selectivity and high sensitivity, and it is applied to a fluorescent sensor, which can realize the specific recognition of copper ions and cadmium ions, and is beneficial to the analysis and detection of copper ions and cadmium ions in the environment. The preparation method is simple, the yield is high, and the reproducibility is good; the raw materials are easy to obtain, the cost is low, the industrial production and application are convenient, and the application prospect is good.

Description

technical field [0001] The invention belongs to the technical field of metal ion analysis and detection, and specifically relates to a C 3 A symmetric fluorescent molecular probe, a preparation method thereof, and applications for detecting copper ions and cadmium ions. Background technique [0002] Fluorescent molecular probes refer to the introduction of fluorescent molecules into the system to be tested through special design, and the information of molecular recognition is converted into fluorescent signals and transmitted to the outside world, so that the dialogue between humans and molecules becomes possible, and at the same time, it also bridges the gap between the macroscopic world and the environment. A bridge to the microcosm. In recent years, fluorescent molecular probes have been highly valued due to their advantages such as good selectivity, high sensitivity, low cost, easy operation, fast response time, and in situ detection. [0003] In analytical chemistry...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06C07D215/12G01N21/64
Inventor 臧双全姜秀娟董喜燕陈会民
Owner ZHENGZHOU UNIV
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