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Transgened-tobacco for degrading organic mercury pollution

An amino acid and nucleotide sequence technology, applied in the fields of botany and pollution control, can solve problems such as unsatisfactory resistance

Inactive Publication Date: 2008-07-02
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has been found that the resistance to organic mercury in transgenic plants obtained directly from bacterial merA and merB is still unsatisfactory

Method used

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  • Transgened-tobacco for degrading organic mercury pollution
  • Transgened-tobacco for degrading organic mercury pollution
  • Transgened-tobacco for degrading organic mercury pollution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] 1 Materials and methods

[0076] (i) Plant material. The test plant was tobacco (Nicotiana tabacum). Tobacco seeds were sterilized by conventional methods, sown on MS medium, and sterile seedlings were obtained, and 25-30d seedling-age leaves were taken as recipient materials for genetic transformation.

[0077](ii) Strains, plasmids, tool enzymes. The strains include Escherichia coli DH5α, Agrobacterium LBA4404 (U.S. Patent 5659121 and U.S. Patent 5808034), and the cloning vector used in the experiment is T-easy (Promega company product), pBluescript KS (+); the plant gene expression vector is pJR1 (U.S. Patent 5659121 and US Patent 5808034), the above-mentioned plasmids and bacteria are commonly used in this field, and have been disclosed in numerous patent documents or can be purchased through commercial channels. Restriction enzymes and modified enzymes were purchased from Huamei Bioengineering Company.

[0078] (iii) Transformation design, artificial total synt...

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Abstract

The invention was involved in organic mercury lyase merB gene, which was expressed in plants. The gene was achieved by changing the sequence of merB catalysed methyl mercury into ion mercury in the bacteria.Then modified merB gene was transferred into plants so that modified gene plants with strong resistance to organic mercury was achieved. The modified gene plants could be used to degrade pollution of organic mercury in soils.

Description

technical field [0001] The invention belongs to the fields of botany and pollution prevention and control. Specifically, the invention relates to the transformation of the organic mercury lyase merB gene, and the preparation of transgenic plants (especially tobacco) with high resistance to organic mercury pollution by using the transformed merB gene method. The invention also relates to a method for degrading organic mercury pollution by using the transgenic plant. Background technique [0002] Mercury is widely used in chemical industry, paper industry, mining industry and defense industry, and is one of the main elements of soil, water and air heavy metal pollution. There are three forms of mercury in the environment: elemental mercury, ionized mercury, and organic mercury (such as methylmercury). Some microorganisms can convert low-toxicity inorganic mercury into highly toxic methylmercury, whose physiological toxicity is thousands of times that of elemental mercury. F...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/60C12N15/63C12N5/10C12N15/74A01H5/00A01H1/00B09C1/00C02F3/32
Inventor 何玉科沈瑞娟王进游
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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