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Preparation method of agarose magnetic microspheres with uniform particle size

A technology of magnetic microspheres and agarose, applied in chemical instruments and methods, alkali metal oxides/hydroxides, inorganic chemistry, etc., can solve problems such as incomplete coating of nano-magnetic cores and leakage of nano-magnetic cores, and achieve Effect of avoiding residue, narrow particle size distribution, and reducing production cost

Pending Publication Date: 2022-01-21
BEAVERNANO TECH
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  • Description
  • Claims
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Problems solved by technology

However, after experimental verification, the low interfacial tension of the water / water interface in the open literature Particulate Science and Technology, 2018, 36:592-599 makes it easy for the nanomagnetic core to leak from the dispersed phase to the continuous phase, resulting in the nanomagnetic core not fully covered

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  • Preparation method of agarose magnetic microspheres with uniform particle size
  • Preparation method of agarose magnetic microspheres with uniform particle size
  • Preparation method of agarose magnetic microspheres with uniform particle size

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preparation example Construction

[0041] The invention provides a method for preparing agarose magnetic microspheres with a uniform particle size, comprising the following steps:

[0042] A) agarose, inorganic salt and magnetic core are mixed in water, stirred to obtain the dispersed phase;

[0043] Mix polyethylene glycol and interfacial stabilizer in water to obtain a continuous phase;

[0044] The interfacial stabilizer is sodium alginate;

[0045] B) under the condition of stirring, add the dispersed phase into the continuous phase, stir and emulsify, cool and solidify to obtain agarose magnetic microspheres.

[0046] The invention firstly prepares the dispersed phase and the continuous phase, the invention adds the agarose into the pure water, then adds the inorganic salt and the magnetic core, stirs and dissolves, and obtains the dispersed phase.

[0047] In the present invention, the inorganic salt is preferably one or more of sodium chloride, potassium chloride and calcium chloride; the magnetic core...

Embodiment 1

[0071] Preparation of dispersed phase: Weigh 2.0g agarose, 0.25g KCl and 1.10g Fe 3 o 4 @SiO 2 Add the magnetic core to 50mL of pure water, place it in a 95°C oil bath, and stir it mechanically at 300rpm for 40 minutes to dissolve.

[0072] Preparation of continuous phase: Weigh 20g of PEG-20000 solid powder and 0.30g of sodium alginate, add them into 100mL of pure water, put them in a 65°C water bath, and stir them mechanically at 300rpm for 60 minutes to dissolve.

[0073] Emulsification cooling: increase the continuous phase speed to 1500rpm, quickly add the dispersed phase to the continuous phase, emulsify and stir for 30 minutes, then take out the hot water in the water bath, add 20-25℃ cold water to cool quickly for 20 minutes, and centrifuge and wash 3 times at 3000rpm. The morphology of the obtained agarose magnetic microspheres was observed through an optical microscope, and the particle size distribution of the agarose magnetic microspheres was detected by a laser ...

Embodiment 2

[0076] Preparation of dispersed phase: Weigh 2.0g agarose, 0.25g KCl and 1.10g Fe 3 o 4 @SiO 2 Add the magnetic core to 50mL of pure water, place it in a 95°C oil bath, and stir it mechanically at 300rpm for 40 minutes to dissolve.

[0077] Preparation of continuous phase: Weigh 20g of PEG-20000 solid powder and 0.15g of sodium alginate, add them into 100mL of pure water, put them in a 65°C water bath, and stir them mechanically at 300rpm for 60 minutes to dissolve.

[0078] Emulsification cooling: increase the continuous phase speed to 1500rpm, quickly add the dispersed phase to the continuous phase, emulsify and stir for 30 minutes, then take out the hot water in the water bath, add 20-25℃ cold water to cool quickly for 20 minutes, and centrifuge and wash 3 times at 3000rpm. The morphology of the obtained agarose magnetic microspheres was observed through an optical microscope, and the particle size distribution of the agarose magnetic microspheres was detected by a laser ...

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Abstract

The invention provides a preparation method of agarose magnetic microspheres with uniform particle size. The preparation method comprises the following steps: A) mixing agarose, inorganic salt and a magnetic core in water, and stirring to obtain a dispersed phase; and mixing polyethylene glycol and an interface stabilizer in water to obtain a continuous phase, wherein the interface stabilizer is sodium alginate; and B) adding the dispersed phase into the continuous phase under a stirring condition, stirring and emulsifying, and cooling and curing to obtain the agarose magnetic microspheres. An interface precipitation method is utilized to form a precipitation layer on the interface of water / water emulsion droplets, so that migration of the Fe3O4@SiO2 magnetic core is remarkably inhibited, meanwhile; due to the existence of the interface precipitation layer, the stability of the water / water emulsion droplets is greatly improved, and the particle size of the magnetic agarose microspheres is more uniform.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, in particular to a method for preparing agarose magnetic microspheres with uniform particle diameters. Background technique [0002] As a special biomolecule immobilization carrier, agarose magnetic microspheres can covalently bind biological ligands such as peptides, proteins, oligonucleotides, etc. to the surface of the microspheres under the action of special chemical reagents (such as EDC). , It can also achieve rapid separation with the help of the magnetic field, thereby greatly reducing the operation steps and time. Therefore, it is an important carrier tool in medical and molecular biology research, and has a wide range of applications in the fields of cell separation, protein purification, and nucleic acid separation. [0003] Existing agarose magnetic microspheres are mainly prepared by emulsification-solidification method, and its main steps are heating and dissolving the agarose ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/28B01J20/30
CPCB01J20/103B01J20/06B01J20/24B01J20/28009
Inventor 尚超陈历历黄明贤任辉
Owner BEAVERNANO TECH