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Selective Magnetic Solid Phase Extraction of Green Tea Polyphenols

A green tea polyphenol, selective technology, applied in the direction of selective adsorption, chemical instruments and methods, other chemical processes, etc., can solve the problems of incapable of magnetic particle encapsulation, low formation efficiency, increase extraction cost, etc., and achieve magnetic enrichment and separation. The effect of simplicity, high utilization rate and improved enrichment efficiency

Active Publication Date: 2020-05-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional polymer encapsulation method for magnetic particles is not efficient, and cannot be effectively wrapped on the surface of magnetic particles, resulting in waste of materials and environmental pollution.
As a result, when extracting green tea polyphenols, the extraction efficiency is not high, and the extraction cost is increased

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 40 mg of Fe3O4 nanoparticles were stirred and mixed with an aqueous solution (1 mmol / mL) containing 2 mg of tannic acid (stirring time 10 min), and the tannic acid was coated on the surface of the magnetic particles (TLC monitored the total adsorption of tannic acid). Then add 1.2 ml of water, N-vinylpyrrolidone (1.33 mmol), N-isopropylacrylamide (0.67 mmol), ethylene glycol dimethacrylate (0.16 mmol), azobisisobutyl Nitrile (0.05 mmol), sodium dodecylbenzenesulfonate (1 mg), nitrogen protection. Put it on a thermo-oscillating reactor and heat it to 60 degrees Celsius for oscillating reaction for 1 hour, remove the supernatant, wash 3 times with ethanol, and then wash 5 times with water to obtain magnetic solid-phase extractant particles.

Embodiment 2

[0034] 40 mg of Fe3O4 nanoparticles were stirred and mixed with an aqueous solution (1 mmol / mL) containing 3 mg of tannic acid (stirring time 10 min), and the tannic acid was coated on the surface of the magnetic particles (TLC monitored the total adsorption of tannic acid). Then add 1.2 ml of water, N-vinylpyrrolidone (1.33 mmol), N-isopropylacrylamide (0.8 mmol), ethylene glycol dimethacrylate (0.16 mmol), azobisisobutyl Nitrile (0.05 mmol), sodium dodecylbenzenesulfonate (1.5 mg), nitrogen protection. Put it on a thermo-oscillating reactor and heat it to 60 degrees Celsius for oscillating reaction for 1 hour, remove the supernatant, wash 3 times with ethanol, and then wash 5 times with water to obtain magnetic solid-phase extraction agent particles, that is, magnetic solid-phase extraction adsorbent.

Embodiment 3

[0036] 40 mg of Fe3O4 nanoparticles were stirred and mixed with an aqueous solution (1 mmol / mL) containing 5 mg of tannic acid (stirring time 10 min), and the tannic acid was coated on the surface of the magnetic particles (TLC monitored the total adsorption of tannic acid). Then add 1.2 ml of water, N-vinylpyrrolidone (1.33 mmol), N-isopropylacrylamide (0.8 mmol), ethylene glycol dimethacrylate (0.16 mmol), azobisisobutyl Nitrile (0.05 mmol), sodium dodecylbenzenesulfonate (1 mg), nitrogen protection. Put it on a thermo-oscillating reactor and heat it to 60 degrees Celsius for oscillating reaction for 1 hour, remove the supernatant, wash 3 times with ethanol, and then wash 5 times with water to obtain magnetic solid-phase extractant particles.

[0037] The magnetic solid phase extraction agent FT-IR test data that embodiment 1~3 obtains shows that 3300-3500cm -1 N-H stretching vibration peak, 1653cm -1Characteristic amide carbonyl peak. It shows that the amide monomer has ...

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Abstract

The invention discloses a selective magnetic solid phase extraction method of green tea polyphenol. The method comprises the following steps: adsorbing a green tea polyphenol solution by utilizing a magnetic solid phase extraction adsorbent, separating the adsorbent after adsorption by utilizing magnetic field force, and performing desorption to obtain purified green tea polyphenol, wherein the magnetic solid phase extraction adsorbent is a polymer adsorbent containing amido bonds, which takes magnetic Fe3O4 nanoparticles as a core, takes tannic acid as an intermediate adsorption layer and takes a polymer containing amido bonds as a shell. The method disclosed by the invention utilizes the magnetic solid phase extraction adsorbent with magnetism to adsorb the green tea polyphenol solution,a large number of amido bonds are distributed on the surfaces of magnetic microspheres, and the amido bonds have a selective adsorption effect on phenolic substances, so that the magnetic microsphereadsorbent is utilized for realizing enrichment, purification of polyphenol is also realized, and non-phenolic impurities are removed.

Description

technical field [0001] The invention belongs to the field of food and medicine processing, and in particular relates to a selective magnetic solid-phase extraction method for green tea polyphenols. Background technique [0002] As an important active ingredient in green tea, green tea polyphenols have the functions of antibacterial, anti-oxidation, anti-aging, anti-bacterial, anti-virus, lowering blood fat, and anti-cancer. Green tea polyphenols have excellent antioxidant effects, and can effectively balance free radicals in the human body by scavenging active oxygen species or reacting to form stable compounds, and their metal ion chelating ability can terminate free radical chain reactions. A large number of in vivo and in vitro experiments and epidemiological data show that tea polyphenols have biological and pharmacological effects such as anti-inflammation, lipid-lowering, anti-diabetes and cardiovascular disease (CVD) prevention. High-tech fields such as fine chemical...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D15/08B01J20/26B01J20/28B01J20/30
CPCB01D15/08B01J20/06B01J20/22B01J20/264B01J20/28009B01J20/28021B01J2220/46B01J2220/4806B01J2220/4812
Inventor 刘松柏蔡赫
Owner ZHEJIANG UNIV
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