Point diffraction digital holographic microscopy device based on multimode fiber

Abstract

The invention discloses a point diffraction digital holographic microscopy device based on multimode fiber illumination. The device comprises a partially coherent light generation module, a telescope system, an object parameter light separation module and an image acquisition module, and the partially coherent light generation module is used for generating partially coherent illumination light; a laser is included, a first microscope objective, a ground glass sheet, an imaging unit and a multimode optical fiber unit which are sequentially arranged along a light path of the laser are included too, and the ground glass sheet is arranged perpendicular to an optical axis and can rotate around the optical axis to generate partially coherent light; the telescope system is used for obtaining object light wave field distribution with sample information; the object parameter light separation module is used for carrying out diffraction light splitting on object light waves with sample information from the telescope system to generate object light and reference light with orthogonal polarization directions; and the image acquisition module is used for acquiring a hologram generated by the object light and the reference light. The microscopy device has the advantages of real-time amplitude / phase quantitative microscopic imaging, good vibration resistance, high signal-to-noise ratio, simple structure and the like.

Description

technical field [0001] The invention belongs to the technical field of microscopic imaging, and in particular relates to a point-diffraction digital holographic microscopic device based on a multimode optical fiber, which can be used to measure the three-dimensional shape or refractive index distribution of tiny objects. Background technique [0002] Digital Holographic Microscopy (DHM), as a quantitative phase imaging technology, combines digital holographic technology with optical microscopy technology, and can quantitatively acquire cells in intensity and phase images obtained by reconstructing holograms. It is an effective full-field quantitative, non-destructive, non-contact, fast, and high-resolution 3D imaging technology. At present, DHM is widely used in industrial inspection, biomedical imaging, special beam generation, gas fluid visualization, and adaptive imaging and other fields. [0003] Although digital holographic microscopy has advantages that traditional op...

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Problems solved by technology

However, LED is a quasi-extended light source, and the size of its light-emitting point is on the order of millimeters, which leads to aliasing of the object light spectrum.

When the pinhole is used to filter the object light spectrum to generate reference light that does not contain sample information, if the pinhole is too large, the reference light will contain sample information; if the pinhole is too small, the reference light intensity will be small and the fringe contrast will be too low

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