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Method for culturing mould dehydrogenated substance seed solution

A cultivation method and seed technology, applied in the biological field, can solve the problems of low total yield, long process route, complicated process, etc., and achieve the effect of high-efficiency production

Pending Publication Date: 2022-01-28
丽江映华生物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Mold dehydrogenation is an intermediate for the preparation of steroid hormones. Mold dehydrogenation is mainly prepared by chemical synthesis and biosynthesis. Chemical synthesis has problems such as long process route, special reaction, complicated process, and low total yield. , biosynthesis is an emerging preparation method, which is still in the research stage, and there are still many key issues to be solved, one of which is how to quickly, safely and efficiently produce mold dehydrogenated seed liquid in large quantities for the production of mold Dehydrogenate

Method used

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  • Method for culturing mould dehydrogenated substance seed solution
  • Method for culturing mould dehydrogenated substance seed solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. Preparation of seed liquid culture medium

[0042] 1.1. Open the lid of the seed tank and check whether the inside of the seed tank is clean and there is water. If there is water, drain it from the bottom and rinse it with process water; the process water is sterilized water.

[0043] 1.2. Check whether the valves of each pipeline are in good condition and whether the mechanical equipment is normal;

[0044] 1.3. Check whether the received raw materials are in good condition, whether the packaging is damaged, whether the weight is consistent with the ingredient list, etc.;

[0045] 1.4. Weigh 32kg of glucose, 56kg of corn steep liquor, 20kg of peptone, 2kg of yeast extract and 2kg of potassium dihydrogen phosphate according to the ratio;

[0046] 1.5. Open the process water valve, put in the process water according to the ratio to the mark of the required amount, and consider the condensed water of the steam when putting in the process water. The total amount of wat...

Embodiment 2

[0076] Other steps in this embodiment are the same as in Embodiment 1, the difference is:

[0077] 1.4. Weigh 32kg of glucose, 54kg of corn steep liquor, 20kg of peptone, 2kg of yeast extract and 2kg of potassium dihydrogen phosphate according to the ratio;

[0078] 1.7. Take a small amount of process water to rinse the mouth of the seed tank and the raw material powder on the inner wall of the tank. After washing, seal the lid of the seed tank, raise the temperature to 40°C, and adjust the pH value of the solution in the seed tank to 8.5 with 20% aqueous sodium hydroxide solution , add 1.2kg of foam enemy to obtain the seed liquid culture medium, tighten the lid of the seed tank, and prepare for sterilization;

[0079] 2.6. When the temperature of the seed liquid culture medium rises to 112°C and the pressure of the seed tank is 0.1 MPa, close the steam inlet valves, close the exhaust valve, keep the temperature and pressure for 20 minutes, and sterilize the seed liquid cultu...

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Abstract

The invention discloses a method for culturing a mould dehydrogenated substance seed solution. The method comprises the following steps: preparing a seed solution culture medium; sterilizing the seed solution culture medium; inoculating the seed solution culture medium; and culturing the seed solution culture medium to obtain the mould dehydrogenation substance seed solution. Glucose, corn steep liquor, peptone, steep liquor, water, yeast extract and monopotassium phosphate are selected as raw materials of the seed solution culture medium, the dosage of the raw materials is adjusted through mass production, when the weight ratio of the raw materials is 32: 54 to 56: 20: 1.2: 4000: 2: 2, the yield of the mold dehydrogenated substance seed solution is the highest, and the mold dehydrogenated substance seed solution can be rapidly, safely and efficiently industrially produced on a large scale.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a method for cultivating mold dehydrogenated seed liquid. Background technique [0002] Mold dehydrogenation is an intermediate for the preparation of steroid hormones. Mold dehydrogenation is mainly prepared by chemical synthesis and biosynthesis. Chemical synthesis has problems such as long process route, special reaction, complicated process, and low total yield. , biosynthesis is an emerging preparation method, which is still in the research stage, and there are still many key issues to be solved, one of which is how to quickly, safely and efficiently produce mold dehydrogenated seed liquid in large quantities for the production of mold Dehydrogenate. Contents of the invention [0003] In view of the above problems, the invention discloses a method for cultivating mold dehydrogenated seed liquid, comprising the following steps: [0004] Preparation of seed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P1/02C12R1/645
CPCC12N1/14C12P1/02
Inventor 谭志云王钱钢杨宗禄谭建权
Owner 丽江映华生物药业有限公司