Disinfection method of hepialus armoricanus eggs

A disinfection method, bat moth technology, applied in botany equipment and methods, horticulture, plant cultivation, etc., can solve problems such as microbial contamination, and achieve the effect of short disinfection time

Pending Publication Date: 2022-02-01
力源新资源开发(广东)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of this invention is to provide a kind of bacterium, fungus and microsporidia that can effectively kill bat moth ovum surface, keep the normal hatching rate of ovum the aseptic method of the bat moth egg surface, thereby solve bat moth insect large-scale breeding The problem of microbial contamination from egg surfaces in

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Egg Elimination Solution A: Prepare an aqueous solution containing 2.4% by mass of sodium hydroxide and 5% by volume of sodium hypochlorite with sterile water in an ultra-clean workbench.

[0020] Egg-killing solution B: In a biological safety cabinet, prepare an aqueous solution containing 37% formaldehyde with sterile water.

[0021] Disinfection of bat moth eggs: put the eggs produced by the females of the small golden moth in a petri dish (directly = 9cm) with wet filter paper on the pad, and place them at 12°C; before disinfection, collect and put the eggs in a biological safety cabinet In a stainless steel screen (the volume of the screen is selected according to the number of sterilized eggs), with sterile PBS (weigh 8.0g NaCl, 0.2g KCl, 1.44g NaCl 2 HPO 4 , 0.24g KH 2 PO 4 Dissolve in 800mL distilled water, adjust the solution to 7.4 with HCl, and finally add distilled water to make up to 1L to obtain 0.01M PBS buffer solution, sterilize and sterilize for lat...

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PUM

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Abstract

The invention discloses a method for disinfecting hepialus armoricanus eggs. The method is characterized in that at a temperature of 10-16 DEG C, hepialus armoricanus eggs are rinsed with sterile PBS, soaked and disinfected with an egg disinfecting liquid A, rinsed with sterile PBS, soaked and disinfected with an egg disinfecting liquid B and rinsed with sterile PBS to obtain disinfected eggs, wherein the egg disinfecting liquid A is a solution containing sodium hydroxide and sodium hypochlorite, and the egg disinfecting liquid B is a solution containing formaldehyde. According to the method for disinfecting the hepialus armoricanus eggs, bacteria and fungi can be effectively killed (the sterility of the disinfected eggs is detected through a flat plate), the disinfection time is short (about 12 minutes), the temperature range (10-16 DEG C) friendly to egg development is used, the hatching rate of the hepialus armoricanus eggs is not obviously influenced (the hatching rate is not obviously different from that of undisinfected fertilized eggs), and the sterility of the surfaces of the hepialus armoricanus eggs in the artificial cultivation process of cordyceps sinensis is realized.

Description

Technical field: [0001] The invention belongs to the technical field of artificial cultivation of Cordyceps sinensis, and in particular relates to a method for disinfecting bat moth eggs. Background technique: [0002] Cordyceps sinensis is a fungus complex formed by Cordyceps sinensis Ophiocordyceps sinensis infecting bat moth larvae (or worm-shaped sclerotia and subseats formed on the worm-shaped sclerotia), which is a rare biological resource unique to the Qinghai-Tibet Plateau. With the sharp increase in the consumption of Cordyceps sinensis, the output of wild Cordyceps sinensis cannot meet the market demand. In order to protect the plateau ecological environment and precious biological resources, and promote the development of the bio-economy, major breakthroughs have been made in the artificial cultivation of Cordyceps sinensis that simulates the plateau ecology, including the artificial cultivation of the fruiting bodies of Cordyceps sinensis, the low-altitude large-...

Claims

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Application Information

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IPC IPC(8): A61L2/18A01G18/00A01K67/033A61L101/06A61L101/32
CPCA01G18/00A01K67/033A61L2/0088A61L2101/06A61L2101/32
Inventor 曹莉吴华周雯熊佳福韩日畴
Owner 力源新资源开发(广东)有限公司
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