Manganese phosphate nano material and rapid preparation method and application thereof

A manganese phosphate nanometer and rapid technology, applied in the direction of nanotechnology, nanotechnology, chemical instruments and methods, etc., can solve the problems of tissue and organ damage, low activation efficiency of immune cells, cytokine storm, etc., to achieve safe and controllable conditions, Good biocompatibility, good effect of biocompatibility

Active Publication Date: 2022-02-01
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method also has disadvantages: on the one hand, a large amount of cytokines are secreted, which can easily cause a cytokine storm, resulting in damage to normal tissues and organs
On the other hand, immune cell activation is inefficient

Method used

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  • Manganese phosphate nano material and rapid preparation method and application thereof
  • Manganese phosphate nano material and rapid preparation method and application thereof
  • Manganese phosphate nano material and rapid preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0037] 1) Take 250 μL of MnCl with a concentration of 1M 2 The solution was added into 4750 μL DMEM solution for reaction, and the reaction was carried out at 37° C. After 10 minutes of reaction, the solution turned into a turbid reaction liquid. The theoretical final concentration of manganese ions in the solution system is 50 mM.

[0038] 2) The reaction solution obtained in step 1) was centrifuged at 8000 rpm for 10 min, the precipitate was washed with deionized water and absolute ethanol, repeated three times, and dried at 50° C. to obtain spherical manganese phosphate nanomaterials of 30 nm.

[0039]Gained spherical manganese phosphate nanomaterials are solid amorphous, and the particle diameter is 30nm, such as figure 1 , Figure 4 , Figure 7 Shown are its thermal field emission scanning electron microscope (FE-SEM) picture, transmission electron microscope (TEM) picture and energy spectrum analysis (EDS) picture, as figure 1 , Figure 4 As shown, thermal field emi...

Embodiment 2

[0041] 1) Take 5mL of MnCl with a concentration of 1M 2 The solution was added into 495 mL of DMEM solution for reaction, and the reaction was carried out at 37°C. After 1 hour of reaction, the solution turned into a turbid reaction liquid. The theoretical final concentration of manganese ions in the solution system is 10 mM.

[0042] 2) The reaction solution obtained in step 1) was centrifuged at 8000 rpm for 10 min, the precipitate was washed with deionized water and absolute ethanol, repeated three times, and dried at 50° C. to obtain 100 nm spherical manganese phosphate nanomaterials.

[0043] Gained spherical manganese phosphate nanomaterials are solid amorphous, and the particle diameter is 100nm, such as figure 2 , Figure 5 , Figure 8 Shown are their thermal field emission scanning electron microscope (FE-SEM) images, transmission electron microscope (TEM) images and energy dispersive spectroscopy (EDS) images, respectively. like figure 2 , Figure 5 As shown,...

Embodiment 3

[0045] 1) Take 10mL of MnCl with a concentration of 1M 2 Added into 490mL of DMEM solution for reaction, the reaction was carried out at 37°C, and after 2 hours of reaction, the solution turned into a turbid reaction liquid. The theoretical final concentration of manganese ions in the solution system is 20mM.

[0046] 2) Centrifuge the reaction solution obtained in step 1) at 8000 rpm for 10 min, wash the precipitate with deionized water and absolute ethanol, repeat three times, and dry at 50° C. to obtain 200 nm spherical manganese phosphate nanomaterials.

[0047] Gained spherical manganese phosphate nanomaterial is solid amorphous, and the particle diameter is 200nm, such as image 3 , Image 6 , Figure 9 Shown are their thermal field emission scanning electron microscope (FE-SEM) images, transmission electron microscope (TEM) images and energy dispersive spectroscopy (EDS) images, respectively. like image 3 , Image 6 As shown, thermal field emission scanning elect...

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Abstract

The invention discloses a manganese phosphate nano material and a rapid preparation method and application thereof, and the rapid preparation method comprises the following steps: adding a manganese-containing inorganic salt solution into a basic culture medium, and carrying out mineralization reaction at 25-42 DEG C for a certain time; and centrifuging the obtained reaction liquid, collecting, washing and precipitating to obtain the manganese phosphate nano material. The manganese phosphate nano material is prepared by adopting a biomimetic mineralization strategy, the method is simple, conditions are safe, mild and controllable, the prepared manganese phosphate nano material is uniform in size and morphology, and cell experiments prove that the prepared manganese phosphate nano material has good biocompatibility.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a manganese phosphate nanomaterial and a rapid preparation method and application thereof. Background technique [0002] Inorganic minerals widely exist in nature, such as plants and animals can spontaneously form minerals (Gower LB.Biomimetic Model Systems for Investigating the Amorphous Precursor Pathway and Its Role in Biomineralization[J].Journal of Cheminformatics,2009,40(5) :4551-4627.). In organisms, with the participation of organic matter, the interaction between organic macromolecules and inorganic ions at the interface to form inorganic minerals is called the biomineralization process. Inspired by biomineralization, scientists have designed and developed materials with different biomimetic functions, which can regulate the formation of inorganic materials through biomimetic mineralization. (Chen W, Wang GH, Yung B. etal. Long-Acting Release Formulat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B25/37B82Y30/00B82Y40/00
CPCC01B25/377B82Y30/00B82Y40/00C01P2004/32C01P2004/03C01P2004/04C01P2002/72C01P2002/82C01P2002/88C01P2004/64C01P2004/62
Inventor 赵瑞波王宇馨孔祥东曹金平张权钟达亮祖柏尔
Owner ZHEJIANG SCI-TECH UNIV
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