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Mononucleotide mutation and KASP specific primer of pepper phytoene synthase gene and application

A specific and genetic technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve problems such as fruit color changes, and achieve the effects of improving efficiency, shortening identification time, and shortening breeding time

Pending Publication Date: 2022-02-01
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, a mutation at base 709 of the β-carotene hydroxylase 2 (β-CHY2, β-CAROTENEHYDROXYLASE2) gene also leads to changes in fruit color

Method used

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  • Mononucleotide mutation and KASP specific primer of pepper phytoene synthase gene and application
  • Mononucleotide mutation and KASP specific primer of pepper phytoene synthase gene and application
  • Mononucleotide mutation and KASP specific primer of pepper phytoene synthase gene and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Getting of pepper PSY1 gene SNP mutation site and determination of corresponding KASP primers

[0035] Test material

[0036] Taking the hybrid F1 generation of pepper CM334, Pelennial, IVF2014032, H3, 0601m, and its orange fruit mutant IVF2017001 and its hybrid F1 generation materials.

[0037] 2. Phenotype identification

[0038] Judging the mature fruit color of the F1 generation and F2 generation separation population by naked eye observation.

[0039] The results showed that the six hybrid combinations F1 ripe fruit red, F2 generation mature fruit is red and orange, and the ratio 3: 1 (p = 0.5110), in accordance with the monomic gene control.

[0040] 3. Determination of pepper psy1 gene SNP mutation sites and corresponding KASP primers

[0041] (1) Geometric weight sequencing and molecular marking design

[0042] The genetically sequencing sequencing of resistant pepper and IVF2017001 and its F2 generation red and orange single strains, based on Zunla-1, acc...

Embodiment 2

[0061] Example 2 Application of the CO4-1 in the hybrid F2 reputation in the hybrid F2 generation of pepper and IVF2017001

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a mononucleotide mutation and KASP specific primer of a pepper phytoene synthase gene and application. The nucleotide sequence of an SNP mutation site KASP marker of ae pepper Psy1 gene is as shown in SEQ ID No.1, and the 21st basic group of the sequence as shown in SEQ ID No.1 is A or T. The color of the fruit does not need to be confirmed after the pepper fruit is physiologically mature, the color of the mature fruit of the pepper material can be judged only by detecting the DNA of the pepper plant in the seedling stage, and the mononucleotide mutation and KASP specific primer can be widely applied to molecular marker-assisted breeding.

Description

Technical field [0001] The present invention belongs to the field of biotechnology, and the mononucleotide mutation and Kasp specific primers and KASP specific primers and applications involved in chiliobiobiocarbone synthetic enzyme gene. Background technique [0002] A commonly mature fruit of pepper, there are many colors such as red, yellow and orange. Studies have shown that 3 independent genetically genetic points Y, C1 and C2 determine that the pepper fruit is red, yellow, orange, and other colors. Chili fruit red (Y) is dominated to yellow or orange (Y), because the red fruit mainly contains two red pigments and chili, Y sites are considered to control hermitin and pepper jerkin synthesis Genetic site. The chain analysis found that the CCS (Capsanthin-Capsorubin Synthase) of Peplette and Peppercikin Synthesis Gene CCS (Capsanthin-Capsorubin Synthase) was co-separated, thus considered CCS as Y coding gene; octopoemene synthase synthase gene psy (phytoe synthase) and C2 Cou...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2600/172C12Q2531/113C12Q2563/107
Inventor 张正海王立浩张宝玺曹亚从于海龙安栋梁
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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