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Reagent for detecting BK virus and JC virus and application

A technology of JC virus and virus, applied in the field of biomedicine, to achieve reliable detection results, high conservatism, and wide coverage

Pending Publication Date: 2022-02-01
领致生物科技(昆山)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the technical problem of how to simultaneously or separately target BKV and JCV for quantitative diagnosis by changing reverse primers

Method used

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  • Reagent for detecting BK virus and JC virus and application
  • Reagent for detecting BK virus and JC virus and application
  • Reagent for detecting BK virus and JC virus and application

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Experimental program
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Effect test

Embodiment

[0024] (1) Select the matching region of BKV and JCV sequences.

[0025] 1) By comparing the existing BKV sequences, multiple highly matching regions of BKV and JCV sequences were found, among which there was a 22bp JCV fragment deletion region in the overlapping region of the late coding region VP2 / VP3 and VP1, so this region was very suitable Design a primer-probe system specifically targeting BKV or JCV, as well as targeting both.

[0026] Select a highly conserved sequence for both BKV and JCV in the matching region to design a universal forward primer, probe and reverse primer targeting both; design a JCV-specific reverse primer near the JCV deletion region; A specific reverse primer specific to BKV was designed in the BKV sequence corresponding to the deletion region. and optimized according to the following criteria:

[0027] A. The size of the amplified fragment is between 80-180bp, and the length of the probe should not exceed 30 nucleotides;

[0028] B. Avoid the ...

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Abstract

The invention relates to a reagent for detecting a BK virus and a JC virus and application, and belongs to the technical field of biological medicines. A section of JCV fragment notch region exists in highly conserved regions of BKV and JCV. A primer probe system for simultaneously targeting BKV and JCV is designed at a suitable position beyond the notch region, and a reverse primer is only designed in the notch region to specifically target BKV or JCV. The reagent is used for preliminary screening, BKV and JCV are targeted at the same time, and resource saving is facilitated; and BKV or JCV can be accurately judged, and the reagent is suitable for clinical popularization. According to the invention, BKV and JCV activation can be prevented, the treatment effect of related diseases can be evaluated in time, the defects and deficiencies of the existing detection system are made up, and the detection result is more reliable. BKV and JCV are taken into consideration, primary screening can be carried out by targeting BKV and JCV at the same time, BKV or JCV can be accurately judged, and timely immunosuppression adjustment is facilitated.

Description

technical field [0001] The invention relates to a reagent for detecting BK virus and JC virus and its application, belonging to the technical field of biomedicine. Background technique [0002] Polyomavirus is a common opportunistic virus with a high carrier rate in the population. Infection is often established and latent in human childhood, and it is activated and causes related diseases when the human body's immunity is low. Among them, BK virus and JC virus (BKV and JCV) are the earliest discovered and best known human polyomaviruses. The carrier rate of BKV is more than 85%, and it does not occur in people with normal immunity. For immunosuppressant users (such as organ transplant recipients, patients with autoimmune diseases, etc.), AIDS patients and other immunocompromised populations, pregnant women and the elderly, BKV may cause serious urinary system diseases, such as 6% of renal Polyomavirus-associated nephropathy (PyVN) occurs in transplant recipients, leading...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/6851C12Q1/6883
CPCC12Q1/701C12Q1/6851C12Q1/6883C12Q2600/118C12Q2531/113C12Q2563/107
Inventor 朱同玉曹懿睿
Owner 领致生物科技(昆山)有限公司
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