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Antheraea pernyi larva low-temperature stress induced resistance breeding method

A technology of low temperature stress and resistance, applied in animal husbandry and other fields, can solve the serious problems of tussah silkworm disease, achieve high reliability and pertinence, ensure high yield, and ensure stability

Active Publication Date: 2022-02-11
辽宁省蚕业科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a kind of tussah silkworm larva low temperature stress-induced resistance breeding method, to solve the tussah silkworm disease caused by the low temperature chilling injury proposed in the above-mentioned background technology serious problem

Method used

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  • Antheraea pernyi larva low-temperature stress induced resistance breeding method

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Experimental program
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Effect test

Embodiment 1

[0028] The tussah silkworm variety Liaocan 582 with high yield, high quality and high feed efficiency was selected as the female parent, and the tussah silkworm variety Kangda with strong disease resistance was selected as the male parent for hybridization to obtain F 1 Generation, F 1 After generation selfing, F 2 -F 7In the segregation population, the moth plots and individuals were selected, and the moth plots were subjected to low temperature stress at 4°C for 24 hours during the 5th instar feeding period, and then they were raised in the wild normally. The moth area and individual seeds are kept for subculture, and the strains are formed through self-cross homozygosity and systematic selection for more than 6 generations. After low-temperature stress identification verification and purebred comparison tests, a new tussah silkworm variety with stronger disease resistance than the parent is obtained, and then it can be used. In the actual production process, the serious p...

Embodiment 2

[0030] F 1 generation, the next generation utilizes F 1 F 2 The moth plots and individuals were selected for the isolated population, and the secondary moth plots were subjected to low temperature stress at 4°C for 24 hours during the 5th instar feeding period, and then they were raised in the wild. The moth area and individual offspring, F 3 -F 7 generation repeat F 2 During the breeding process of the first generation, a strain was formed through homozygous selfing and systematic selection for more than 6 generations, and a new tussah silkworm variety with stronger disease resistance than the parent was obtained through low temperature stress identification verification and purebred comparison test. figure 2 It can be seen that the tolerance to low temperature climate conditions is strong, and the incidence rate is low. Under the low temperature stress condition, the incidence rate of pustular disease is 67.23 percentage points lower than that of the non-selected line, ...

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Abstract

The invention discloses an antheraea pernyi larva low-temperature stress induced resistance breeding method, and relates to the technical field of antheraea pernyi breeding. The method comprises the following step: S1, parent matching: carrying out parent screening, namely selecting high-yield, high-quality and high-feed conversion efficiency Liao silkworm 582 as a female parent, and selecting an antheraea pernyi variety with strong disease resistance as a male parent, and preparing the selected female parent and male parent into a hybrid combination. According to the method, the modes of excellent variety hybridization and excellent variety selection are set, low-temperature stress screening pressure is applied to the antheraea pernyi larva by simulating low-temperature cold damage climate conditions on the basis of manual selection from the F2 generation, and 6 generations are applied so that the method has higher reliability and pertinence aiming at the breeding target; and therefore, the problem that tussah diseases are serious due to low temperature and cold damage when the tussah is bred in the field tussah garden is solved, and meanwhile the stability and the high yield of the stocking yield of the tussah in the field tussah garden are guaranteed.

Description

technical field [0001] The invention relates to a method for cultivating tussah larvae, in particular to a method for breeding resistance of tussah larvae induced by low temperature stress. Background technique [0002] Tussah silkworm (Antheraea pernyi) belongs to the family Lepidoptera, which originated in China. It is a unique silk-producing insect with high economic value in my country. Its cocoon production accounts for more than 90% of the world's total, and it has become a key silkworm area. It is an important part of agricultural production and the main source of income for farmers in some mountainous areas. At present, tussah silkworms in various development stages can be used in many fields such as food, medicine, biological control intermediate host, and bioreactor. [0003] Tussah silkworms are stocked in wild tussah gardens, which are greatly affected by abiotic factors (cold damage, strong wind, heavy rain, high temperature, drought, sun exposure, pesticide pol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/04
CPCA01K67/04
Inventor 徐亮钟亮焦阳姜晓旭
Owner 辽宁省蚕业科学研究所
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