Method for treating cerebral ischemia-reperfusion injury by using dental pulp stem cell exosome
A technology of cerebral ischemia reperfusion and dental pulp stem cells, which is applied in the field of dental pulp stem cell therapy, can solve the problems of limiting clinical application and damage to the body, and achieve the effect of reducing the size of cerebral infarction
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[0028] Example 1, the method of using dental pulp stem cell exosomes to treat cerebral ischemia-reperfusion injury, the specific steps are
[0029] S1: Exosomes derived from dental pulp stem cells were extracted from healthy volunteers aged 18 to 30 years without caries and periodontal lesions from third molars - 75% alcohol disinfection of the tooth surface - dental drill to remove the pulp - Cut into small pieces of 1 mm3 - Rinse 3 times with PBS containing 2.5% antibiotics - Put the pulp tissue in a 1.5ml EP tube and digest it with 3mg / ml type I collagenase - 4mg / mL dispase at 37°C for 30 minutes - Cells were resuspended - cultured in a 37°C 5% CO2 incubator (medium: DMEM + 10% fetal bovine serum + 100U / mL penicillin and 100ug / mL streptomycin) - change the medium after 5 days, and then every three days Once; when the cells are fused to about 80%, digest and centrifuge, and transfer to the third generation; when the third generation cells are fused to about 80%, remove the u...
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