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Fatty liver detection reagent and application thereof in fatty liver detection

A technology for detecting reagents and fatty liver, which is applied in the field of biomedicine, can solve problems such as low repeatability, complicated equipment operation, and high sensitivity, and achieve high specificity, accuracy, good stability, and simple operation

Pending Publication Date: 2022-02-18
JIANGSU XIANSIDA BIOTECH CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the mass spectrometry instrument is precise, the operation of the equipment is complicated, and the mass spectrometer is expensive, so it is not suitable for popularization and use in clinical practice; (3) capillary electrophoresis (CE): capillary electrophoresis is low in cost, high in column efficiency, high in sensitivity, fast in speed, and easy to inject. The amount is small and the operation is simple, but the repeatability is not high and the stability is not as good as HPLC

Method used

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  • Fatty liver detection reagent and application thereof in fatty liver detection
  • Fatty liver detection reagent and application thereof in fatty liver detection

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Experimental program
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Effect test

Embodiment 1

[0051] Embodiment 1 detects fatty liver

[0052] Statistical analysis was carried out by measuring the G-Test specific fingerprint of serum glycoprotein oligosaccharide chains, and the materials and methods used:

[0053] 1. Test samples: collect serum from patients with fatty liver and normal controls.

[0054] 2. Experimental equipment: sugar group analyzer, PCR, centrifuge.

[0055] 3. Reagent preparation:

[0056] Reagent A: prepared by adding SDS with a mass concentration of 0.5 to 5% in ammonium bicarbonate solution with a concentration of 10 mM;

[0057] Reagent B: It is prepared by mixing 0.01~10U / 10μL glucosamidase and 0.01~10U / 10μL sialidase, and the pH value of the mixed solution is 4~9;

[0058] Reagent C: Prepared by dissolving 8-aminopyrene-1,3,6-trisulfonic acid in DMSO, the concentration is 0.01mM~1M;

[0059] Reagent D: stop solution.

[0060] 4. Glycosequencing detection steps:

[0061] Step 1 Preparation of oligosaccharide chains

[0062] Add 4 μL of ...

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Abstract

The invention provides a fatty liver detection reagent and a preparation method thereof, and the detection reagent is prepared by mixing the following reagents: a reagent A, which is prepared by adding 0.5-5% by mass of SDS into an ammonium bicarbonate solution with a concentration of 10mM; a reagent B, which is prepared by mixing 0.01-10U / 10 microL of glycosamine acylase and 0.01-10U / 10microL of sialidase, and the pH value of the mixed solution is 4-9; a reagent C, which is prepared by dissolving 8-aminopyrene-1, 3, 6-trisulfonic acid in DMSO (dimethylsulfoxide), and the concentration of the reagent C is 0.01mM to 1M; and a reagent D: a stop solution. According to the invention, a glycome map in serum is determined by a detection reagent, and a peak value is quantified for statistical analysis, so that a method for establishing a model of a fatty liver serum glycome map is provided for detecting fatty liver.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a method for detecting fatty liver, in particular to a method for detecting fatty liver based on the specific fingerprint of serum glycoprotein oligosaccharide chain detection (G-Test). Background technique [0002] Fatty liver refers to the lesion of excessive fat accumulation in liver cells due to various reasons, resulting in lipid accumulation in liver cells exceeding 5% of the wet weight of the liver, which is called fatty liver (Fatty liver). Fatty liver disease has become the second largest liver disease after viral hepatitis, and its incidence is increasing, and the age of onset is getting younger and younger, which is seriously threatening people's health. Fatty liver is generally divided into alcoholic fatty liver and non-alcoholic fatty liver two categories. According to the extent of fatty degeneration in the liver, it can be divided into three types: mild, moderate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/34
CPCC12Q1/34G01N2333/924G01N2333/98G01N2800/085
Inventor 陈翠英王蕾谈宗男
Owner JIANGSU XIANSIDA BIOTECH CO LTD
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