Cell screening model of unmarked membrane receptor GPR84 and application of cell screening model
A label-free, membrane receptor technology that can be used in compound screening, cells modified by the introduction of foreign genetic material, detection of programmed cell death, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0038] Example 1: DMR characteristic signal spectrum of GPR84 receptor agonist GTPL5846 on HEK293T-Gi3-GPR84 cells
[0039] HEK293T-Gi3-GPR84 human embryonic kidney cells were obtained from Dalian Institute of Chemical Physics, Chinese Academy of Sciences, and the inverted microscope was purchased from OLYMPUS. GTPL5846 (product number: HY-1276 (4) and GPR84 antagonist 8 (product number: HY-11256 (2) were purchased from MedChemExpress Company. Culture medium DMEM (product number: 01-052-1ACS) was purchased from Biological Industries Company. Balanced salt solution HBSS (Cat. No.: 14065-056) and HEPES (Cat. No.: 15630-080) were purchased from Gibco. The cell culture plate was an Epic optical biosensing 384 microwell plate, purchased from Corning. The detection platform was Corning’s third-generation Epic ® Imager, purchased from Corning, whose detection signal is the wavelength shift caused by cell dynamic mass reset (DMR).
[0040] HEK293T-Gi3-GPR84 cells in the logarithmic g...
Embodiment 2
[0041] Example 2: Desensitized DMR characteristic signal spectrum of HEK293T-Gi3-GPR84 cells
[0042] HEK293T-Gi3-GPR84 cells in the logarithmic growth phase were seeded in a cell-compatible 384 microwell plate, the medium used was DMEM, the seeding volume per well was 40 µL, and the seeding density was 2.5×10 4 cells / well, place the inoculated cell plate in a cell culture incubator for 20-24 h, until the cell confluency reaches about 95%, and carry out activity detection. Replace the cell culture medium in the microwell plate with Hank's Balanced Salt Solution (HBSS, containing 20 mM HEPES), and add a volume of 30 μL to each well. After adding, place in Epic ® Equilibrate on the imager for 90 min; add different concentrations of GTPL5846 into the microwell plate to pretreat HEK293T-Gi3-GPR84 cells for 90 min, the volume of each well is 10 μL, the concentration is 1000 nM, 500 nM, 250 nM, 125 nM, 62.5 nM, 31.2 nM, 15.6 nM, 7.8 nM, 3.9 nM, 2.0 nM, 1.0 nM, 0.5 nM, 0.2 nM and 0....
Embodiment 3
[0043] Example 3: DMR characteristic signal spectrum of GPR84 receptor antagonist GPR84 antagonist 8 on HEK293T-Gi3-GPR84 cells
[0044] HEK293T-Gi3-GPR84 cells in the logarithmic growth phase were seeded in a cell-compatible 384 microwell plate, the medium used was DMEM, the seeding volume per well was 40 µL, and the seeding density was 2.5×10 4 cells / well, place the inoculated cell plate in a cell culture incubator for 20-24 h, until the cell confluency reaches about 95%, and carry out activity detection. Replace the cell culture medium in the microwell plate with Hank's Balanced Salt Solution (HBSS, containing 20 mM HEPES), and add a volume of 30 μL to each well. After adding, place in Epic ® Equilibrate on the imager for 90 min; re-scan the baseline for 2 min, add different concentrations of GPR84 antagonist 8 into the microwell plate, the volume of each well is 10 μL, the concentration is 10000 nM, 5000 nM, 2500 nM, 1250 nM, 625 nM , 312.5 nM, 156.2 nM, 78.1 nM, 39.0 nM,...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap