Biomarker with poor prognosis of multiple myeloma, screening method, prognosis layering model and application
A multiple myeloma, biomarker technology, applied in biochemical equipment and methods, bioinformatics, microbial determination/examination, etc., can solve problems such as insufficient median survival time, poor curative effect, etc. The effect of short and auxiliary clinical diagnosis and treatment decision-making
Pending Publication Date: 2022-03-04
INST OF HEMATOLOGY & BLOOD DISEASES HOSPITAL CHINESE ACADEMY OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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Problems solved by technology
The existing treatment mode includes chemotherapy induction therapy (VRD scheme), combined with double autologous hematopoietic stem cell transplantation, the curative effect is still not good for 15% to 25% of very high-risk patients, and these patients have rapid tumor growth and rapid disease progression. Less than 3 years of survival
Due to the lack of effective biomarkers, how to early identify and diagnose these high-risk and high-invasive patients, especially ultra-high-risk patients, and give them early and standardized stratified treatment is still a challenge in current clinical work
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Embodiment 1
[0045] Bone marrow samples were collected from 12 newly diagnosed MM patients and 7 healthy controls, and the mononuclear cells were obtained by Ficoll separation; Beijing Nuohezhiyuan Technology Co., Ltd. was entrusted with single-cell sequencing to analyze the newly diagnosed MM from the single-cell level The composition and proportion of mononuclear cells in the patient's bone marrow were analyzed, and the gene expression of the tumor cells was analyzed.
[0046] At the same time, another 58 newly diagnosed high-risk MM patients (currently sMART3.0, Mayo Clinic2018 standard high-risk) bone marrow samples were collected, and MM cells were purified by Ficoll combined with CD138 immunomagnetic bead sorting method, and transcriptome sequencing (bulkRNAseq) was performed. The clinical information of the above patients was collected.
[0047] After passing the quality inspection, a total of 42,936 cells were subjected to single-cell sequencing. Then unsupervised clustering was u...
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The invention provides multiple myeloma poor prognosis biomarkers, a screening method, a prognosis layering model and application, and relates to the technical field of tumor prognosis. A single cell transcriptome sequencing technology is combined with technologies such as unsupervised clustering, Cox regression analysis and transcriptome sequencing, tumor cell gene expression differences of the MM patient are analyzed, seven genes and biomarkers related to poor prognosis of the patient are obtained through identification, a weighted average calculation formula is established, a model is established through external data, and the prognosis of the patient is evaluated. Verifying internal data; it is proved that seven MM high-risk patients with abnormally increased genes are super-high-risk groups, the survival time is shorter, and prognosis is poor. Clinical application of the combined risk prognosis layering model can be promoted, and clinical diagnosis and treatment decision can be assisted.
Description
technical field [0001] The invention belongs to the technical field of tumor prognosis stratification and prognosis assessment, and in particular relates to multiple myeloma poor prognosis biomarkers and screening methods, as well as establishment and application of a prognostic stratification model optimization. Background technique [0002] Hematological malignancies are one of the common malignant tumors that threaten human health. Domestic epidemiological data show that lymphoma, leukemia and multiple myeloma are the top three hematological tumors in my country with the highest incidence. Although in recent years, due to the wide application of new drugs and the emergence of targeted therapy and immunotherapy, the treatment of multiple myeloma (MM) has made significant progress, and the median survival period of MM patients has been extended to 6-8 years. But MM is still an incurable malignant disease, and almost all patients eventually develop into refractory (RRMM) or...
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Login to View More IPC IPC(8): C12Q1/6886C12Q1/6869C12N15/11G16B30/00
CPCC12Q1/6886C12Q1/6869G16B30/00C12Q2600/118C12Q2600/158Y02A50/30
Inventor 郝牧孙浩邱录贵
Owner INST OF HEMATOLOGY & BLOOD DISEASES HOSPITAL CHINESE ACADEMY OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE