Preparation method of magnolia flower callus culture

A technology for callus and culture, applied in the field of preparation of magnolia callus culture, can solve the problems of limiting the large-scale application of magnolia, low success rate of sexual reproduction, limited application of magnolia, etc. Antioxidant activity and bacteriostatic activity, improving induction efficiency, and preventing browning

Pending Publication Date: 2022-03-11
ZHEJIANG YIGE ENTERPRISE MANAGEMENT GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The Magnoliaceae plant to which Magnolia magnolia belongs is a scarce resource in nature at present. The sexual reproduction success rate of this plant in wild conditions is extremely low. In addition, due to environmental changes and excessive exploitation and utilization by humans, these factors have led to the The current situation of extremely scarce resources, the above-mentioned current situation has limited the large-scale application of magnolia flower in the field of cosmetics
Therefore, there is the problem that magnolia flower application is limited in existing technology

Method used

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  • Preparation method of magnolia flower callus culture
  • Preparation method of magnolia flower callus culture
  • Preparation method of magnolia flower callus culture

Examples

Experimental program
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Embodiment 1

[0025] Example 1. A preparation method of magnolia flower callus culture, comprising the following steps:

[0026] A, choose the stem segment position of Magnolia Magnolia as explant;

[0027] B, the explant is carried out disinfection treatment, obtains the explant that removes bacteria;

[0028] C, carry out callus induction culture to degerm explant, obtain the callus of first generation Magnolia Magnolia; The induction medium used during induction culture includes the following components: MS medium, 2,4D, 6-BA, NAA , peptide flavin and sucrose;

[0029] D. The callus of Magnolia Magnolia was subcultured to obtain the callus of Magnolia Magnolia; the medium used in the subculture included the following components: MS medium, 6-BA, NAA, VC and sucrose.

[0030] In the induction medium used for induction culture: 2,4D is 4mg / L; 6-BA is 1mg / L; NAA is 4mg / L; peptide flavin is 0.1mg / L; sucrose is 30g / L.

[0031] In the medium used for subculture: 6-BA is 1 mg / L; NAA is 4 mg...

Embodiment 2

[0043] Example 2. A preparation method of magnolia flower callus culture, comprising the following steps:

[0044] Selection and Preparation of Magnolia Magnolia Flower Explants

[0045]Select the stem section of Magnolia Magnolia plucked in December (this part grows the most vigorously and is the preferred part for inducing callus), and the obtained explants are fully disinfected. The disinfection treatment method is as follows: the obtained explants are placed in a beaker and soaked in sterile water for 1 day. Afterwards, the explant was fully soaked in 5% NaClO solution, and oscillated for 30 minutes to sterilize the surface of the explant. Afterwards, the whole explant after the 5% NaClO solution was fully sterilized was transferred to a sterile workbench, and the explant was taken out. Rinse off with sterile water. Then soak it in 75% ethanol for 30s, take it out and rinse it with sterile water for 1min, then repeat this operation twice. The seeds treated with 75% et...

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Abstract

The invention discloses a preparation method of a magnolia flower callus culture. The preparation method comprises the following steps: A, selecting a stem part of a magnolia flower as an explant; b, performing disinfection treatment on the explant to obtain a degerming explant; c, performing callus induction culture on the sterilized explants to obtain primary magnolia grandiflora callus; an induction culture medium used during induction culture comprises the following components: an MS culture medium, 2, 4D, 6-BA, NAA, peptidoflavin and cane sugar; d, carrying out subculture on the southern magnolia callus to obtain the southern magnolia callus; a culture medium used during subculture comprises the following components: an MS culture medium, 6-BA, NAA, VC and sucrose. The method has the characteristic that the application of the magnolia flower in the field of cosmetics can be effectively improved.

Description

technical field [0001] The invention relates to a magnolia flower tissue, in particular to a method for preparing a magnolia flower callus culture. Background technique [0002] Magnolia grandiflora Linn (Magnolia grandiflora Linn) is a tree species of the genus Magnolia in the Magnolia family. The main component of its alcohol extract is flavonoids. Flavonoids are high-quality antioxidants that can effectively remove free radicals produced during the body's metabolism. The total flavonoids of Magnolia Magnolia flower extract contain anthocyanins, which can effectively reduce the overflow of oily peroxides. Experiments show that anthocyanins also have a delaying effect on cell aging, and are high-quality anti-aging raw materials. In addition, Magnolia flavonoids also have obvious effects of expanding blood vessels and lowering blood pressure, and inhibiting platelet aggregation and thrombus formation. Magnolia Magnolia flower extract is a kind that can be widely used in cos...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008
Inventor 张大勇毕永贤孔德承周浩淼李昊杜雨涵胡雪情
Owner ZHEJIANG YIGE ENTERPRISE MANAGEMENT GRP CO LTD
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