Micro-fluidic chip for nucleic acid purification and nucleic acid purification method

Abstract

The invention discloses a micro-fluidic chip for nucleic acid purification and a nucleic acid purification method. The micro-fluidic chip comprises an interface layer, a functional layer and a sealing layer, and the interface layer, the functional layer and the sealing layer are packaged into a whole from bottom to top to form the micro-fluidic chip; a first inlet, a second inlet and a discharge port are formed in the interface layer; a reactant is introduced through the first inlet, and a sample is introduced through the second inlet; a bubble capture module, a nucleic acid enrichment and purification module and a micro-channel are formed on the functional layer; and the combination capture, washing, elution and nucleic acid recovery in nucleic acid purification are realized by controlling the types and sequences of reaction reagents and samples flowing through the bubble capture module and the nucleic acid enrichment and purification module from the first inlet and the second inlet. The nucleic acid purification operation is simplified, the reagent consumption in the purification process is reduced, and cross contamination in the purification process can be avoided.

Description

technical field [0001] The invention relates to the technical fields of medicine and biological detection, in particular to a microfluidic chip for nucleic acid purification and a nucleic acid purification method. Background technique [0002] The microfluidic chip is a micro-channel network formed on the chip by using micro-nano processing technology in the micron-scale space range, and the controllable fluid is used as the main operating carrier to reduce the basic functions of various biological and chemical laboratories to a small size. It is implemented on a chip of a few square centimeters. With the development of microfluidics and related technologies, the chip can flexibly combine and scale integrate various unit technologies on a small and controllable platform, which has become the biggest advantage of today's development. In recent years, important biochemical processes such as organ chips, immune protein reactions, nucleic acid amplification reactions, and cell ...

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Problems solved by technology

[0003] Commonly used pathogenic nucleic acid extraction and purification technologies include Trizol method, spin column method, magnetic bead method and other methods. These methods based on artificial nucleic acid extraction face problems such as complex extraction process, long time, low nucleic acid extraction efficiency and poor stability.

In order to solve these problems, many attempts have been made to the structural design of microfluidic chips, but these microfluidic chips can only realize some functions in the nucleic acid extraction process, and do not consider the actual needs of clinical applications, so it is difficult to industrialize

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